FastQCFastQC Report
Wed 25 May 2016
SRR1779880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184379
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT95825.196904202756279No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73673.9955743333025997No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT30971.6796923727756417No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG10880.5900888929867284No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC6450.3498229190959925No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT6260.33951805791331985TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC2730.14806458436155961No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGC150.00222316469.99023412
CACGGGT150.00222316469.99023453
GTTACTC150.00222316469.99023418
GCGTCAC150.00222316469.99023449
CGTCACG150.00222316469.99023450
AGTCGAG150.00222316469.99023411
CCCGACG150.00222316469.99023412
ATTATAC14400.061.7442441
AGGGGGG18000.060.46378770
ATCGTAT352.9650437E-759.9916339
AGCTTGA601.8189894E-1258.32519555
GGGAGCG252.3874013E-455.99218849
CCGTCTT19900.054.163347
CGTCTTC19800.053.9066248
GCCGTCT20100.053.79846246
ATGCCGT20600.053.51195544
TGCCGTC20600.053.51195545
TATGCCG20950.053.45316343
CGTATGC21250.053.3572641
TCGTATG21250.053.19257740