FastQCFastQC Report
Wed 25 May 2016
SRR1779879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221963
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2683712.090753864382803No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG55042.479692561372841No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC10740.4838644278550929No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG6760.3045552637151237No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT5560.25049219915030885No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGCG150.0022229369.9968520
AAAGGTC200.006939732552.50946446
CGACGGG200.006945921652.49763529
GCGCGTT200.006945921652.49763524
GATCATA200.006945921652.4976351
GGCCACA200.006945921652.49763540
GTTCATA200.006945921652.49763534
GTTACCG200.00695211552.48580613
CGGTGGT9600.043.39323846
CTCGGTG9600.043.3834644
TCGCCGT9550.043.25387252
TGGTCGC9650.043.16840449
TCGGTGG9650.043.15867645
GCCGTAT9500.043.0936154
CGCCGTA9650.042.78635453
GTCGCCG9750.042.7256551
ATCTCGG9750.042.71602242
GATCTCG9750.042.71602241
TCTCGGT9800.042.4980843
CGTATCA9800.042.13146656