FastQCFastQC Report
Wed 25 May 2016
SRR1779879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221963
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95584.306123092587503No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT86583.9006501083513916No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT85103.8339723287214538No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG17330.7807607574235346No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC9770.4401634506652009No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC9350.4212413780675157No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT5410.243734316079707TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC3230.14551974878695997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC12400.062.3746151
TGCCGAC401.0624717E-861.24566345
AGGGGGG20000.060.02075270
CGAATGC308.361434E-658.32920541
GCCGTCA2700.058.32920546
GTGCCTC308.361434E-658.32920545
GCCGACT252.3876561E-455.99603746
CCGACTT252.3876561E-455.99603747
CCGTCAT2900.053.0996947
AAAACGG200.006940442652.50811467
CAAATTG200.006940442652.50811431
GGAGCTA200.00694663152.49628453
CGTCTAC200.00694663152.49628448
ATTCCGT200.00694663152.49628444
TTGTTCG200.00694663152.4962849
ATCGCGC200.00694663152.4962848
TCAGCAT950.051.575353
TATGCCG28450.050.06675743
AAGCACG352.0828558E-549.996467
AATCTCG352.0828558E-549.9964636