FastQCFastQC Report
Wed 25 May 2016
SRR1779878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176151
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1829110.383704889554984No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG40282.2866745008543807No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC11550.6556874499718991No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG4800.27249348570260745No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT4620.26227497998875965No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG2130.12091898428053204No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1890.10729430999540167No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCTT207.9042984E-570.010226
GTTCGAA150.002220482670.0102238
ATGTTCG150.002220482670.010222
GTTATCG308.3576215E-658.3252911
ACGTTAT252.387089E-455.9922839
CGTTATC252.387089E-455.99228310
TATCGTC252.387089E-455.99228313
CGGAATC200.006938306652.50766820
TGCCCCG200.006938306652.50766824
GATGTTC200.006938306652.5076681
CTTATAT200.006938306652.5076682
CGAATTC200.006938306652.50766841
CTCTAGG200.006938306652.5076684
ACCCAGA200.006938306652.50766825
GACTACC200.006938306652.50766841
ACGTTAC200.00694610352.49276431
CGTTACT200.00694610352.49276432
CGTAGCG200.00694610352.49276415
CCGACGT200.00694610352.49276428
GCGGAAT200.00694610352.49276419
Produced by FastQC (version 0.11.5)