FastQCFastQC Report
Wed 25 May 2016
SRR1779878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176151
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT96915.501529937383268RNA PCR Primer, Index 19 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81594.631821562182446No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT10320.585860994260606No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT9080.5154668437874323No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG8090.45926506236126957No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC5150.29236280236842255No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC2850.16179300713592315No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGACGT2480.14078830094634717No Hit
ATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC1820.10332044666223864No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAACG150.002222663569.99290533
ATACCGT150.002222663569.9929051
AACACCG150.002222663569.99290510
ATTATAC15000.060.893821
AGGGGGG14050.057.80414270
GGTGTAA252.3866595E-455.994321
CGCGGTC200.00693730752.50958344
CTTTTGT200.00693730752.50958346
GCTATAC200.006945102552.4946753
ACTTCGA200.006945102552.49467530
AATTGCT200.006945102552.49467518
CGTCTTC17700.052.00037448
CCGTCTT17850.051.95551347
TATGCCG18800.051.95096643
GCCGTCT18150.051.88274846
CGTATGC19100.051.68482641
TCGTATG19450.051.47468640
ATGCCGT18800.051.39235344
CTTGAAA18600.051.36576557
ATCTCGT19100.051.31826837
Produced by FastQC (version 0.11.5)