##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779878_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 176151 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.600433718798076 32.0 22.0 32.0 13.0 32.0 2 28.787279095775784 32.0 27.0 32.0 13.0 32.0 3 29.525282286220346 32.0 32.0 32.0 22.0 32.0 4 30.48172874408888 32.0 32.0 32.0 27.0 32.0 5 30.106720938285903 32.0 32.0 32.0 27.0 32.0 6 33.72780739252119 37.0 32.0 37.0 22.0 37.0 7 33.39908941760194 37.0 32.0 37.0 22.0 37.0 8 32.81818439861255 37.0 32.0 37.0 13.0 37.0 9 33.81163320106045 37.0 37.0 37.0 27.0 37.0 10-11 33.54389415898859 37.0 32.0 37.0 22.0 37.0 12-13 33.36546201838196 37.0 32.0 37.0 22.0 37.0 14-15 33.59395064461741 37.0 32.0 37.0 22.0 37.0 16-17 33.14207980652962 37.0 34.5 37.0 22.0 37.0 18-19 33.444587314292846 37.0 32.0 37.0 22.0 37.0 20-21 33.47680683050338 37.0 32.0 37.0 22.0 37.0 22-23 33.20653870826734 37.0 32.0 37.0 22.0 37.0 24-25 33.209135911802946 37.0 32.0 37.0 22.0 37.0 26-27 33.33529471873563 37.0 32.0 37.0 22.0 37.0 28-29 33.27668307304529 37.0 32.0 37.0 22.0 37.0 30-31 33.173444942123524 37.0 32.0 37.0 22.0 37.0 32-33 32.84505055321854 37.0 32.0 37.0 17.5 37.0 34-35 32.85195372152301 37.0 32.0 37.0 17.5 37.0 36-37 32.84459355893523 37.0 32.0 37.0 17.5 37.0 38-39 32.55619042753092 37.0 32.0 37.0 13.0 37.0 40-41 32.54539571163377 37.0 32.0 37.0 17.5 37.0 42-43 32.825888016531266 37.0 32.0 37.0 13.0 37.0 44-45 32.69867897428911 37.0 32.0 37.0 13.0 37.0 46-47 32.14461456364142 37.0 32.0 37.0 13.0 37.0 48-49 31.91625650720121 37.0 32.0 37.0 13.0 37.0 50-51 31.622369444397137 37.0 27.0 37.0 13.0 37.0 52-53 31.520107748465804 37.0 27.0 37.0 13.0 37.0 54-55 31.401757582982782 37.0 27.0 37.0 13.0 37.0 56-57 30.85111353327543 37.0 27.0 37.0 13.0 37.0 58-59 31.094135145415017 37.0 27.0 37.0 13.0 37.0 60-61 30.690958325527532 37.0 27.0 37.0 13.0 37.0 62-63 30.918595409620156 37.0 27.0 37.0 13.0 37.0 64-65 30.738863248008812 37.0 27.0 37.0 13.0 37.0 66-67 30.406852643470657 37.0 27.0 37.0 13.0 37.0 68-69 29.920656141605782 37.0 27.0 37.0 13.0 37.0 70-71 28.473187776396387 34.5 17.5 37.0 10.5 37.0 72-73 29.037027890843653 37.0 22.0 37.0 10.5 37.0 74-75 29.24943940142264 37.0 22.0 37.0 13.0 37.0 76 28.61147538191665 37.0 22.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.9009437101223838 11101 2 -2.0742532985169113 11101 3 -1.9568205177881772 11101 4 -0.2827447328653925 11101 5 -1.794382079565736 11101 6 -1.6236306867532164 11101 7 -1.5131604735182087 11101 8 -0.2493005699239106 11101 9 -2.504602837972584 11101 10-11 -0.4085828285977229 11101 12-13 -1.4665861096197403 11101 14-15 -0.9951246367668887 11101 16-17 -1.566638863137122 11101 18-19 -1.898703151468304 11101 20-21 -0.9604553976739822 11101 22-23 -0.5110197525324622 11101 24-25 -1.5543152017045117 11101 26-27 -1.1160862607201665 11101 28-29 -1.5740931401897313 11101 30-31 -1.0605931392372874 11101 32-33 -1.5387119056679595 11101 34-35 -1.233072946196888 11101 36-37 -1.074507457716571 11101 38-39 -2.335997193040029 11101 40-41 -1.914613070948647 11101 42-43 -3.147092559643781 11101 44-45 -3.5604780270316603 11101 46-47 -1.6469223370492898 11101 48-49 -1.8018968733473564 11101 50-51 -1.0795061750656743 11101 52-53 -1.8439845698247623 11101 54-55 -2.3538915489218866 11101 56-57 -2.278316760105998 11101 58-59 -1.574029843450127 11101 60-61 -1.5819670271829125 11101 62-63 -1.421193906677793 11101 64-65 -2.4478484364882434 11101 66-67 -0.7099963941473497 11101 68-69 -2.380881096145373 11101 70-71 -0.751154638902026 11101 72-73 -1.086357794464483 11101 74-75 -2.121903702174457 11101 76 -4.574684437833074 11102 1 -0.7075115744304625 11102 2 -0.6498630546144746 11102 3 -0.3095034446174445 11102 4 -1.0567284727027904 11102 5 0.15846344888954889 11102 6 -0.7699721501678525 11102 7 -1.2609653515669876 11102 8 -0.14507292764749025 11102 9 -0.1859036509807126 11102 10-11 -1.5288267310367516 11102 12-13 -0.5830901746603914 11102 14-15 -0.9552872383928985 11102 16-17 -0.6463136598850809 11102 18-19 -1.2472397368341532 11102 20-21 -1.4663903570235703 11102 22-23 -1.1110197525324566 11102 24-25 -0.2878924374768701 11102 26-27 -0.12633016315919576 11102 28-29 -1.9860443597019248 11102 30-31 -0.7195362286681828 11102 32-33 -1.2158663772126772 11102 34-35 0.03082949282750036 11102 36-37 -0.44125542519625327 11102 38-39 -1.7940459735278331 11102 40-41 -0.65469437176165 11102 42-43 -1.7829462181803706 11102 44-45 -2.401778840039789 11102 46-47 -2.392775995585879 11102 48-49 -1.619539149770123 11102 50-51 -1.0460102401063232 11102 52-53 -1.3977244072231372 11102 54-55 -1.4151110611170097 11102 56-57 -1.1466907438458342 11102 58-59 -0.26711927434443794 11102 60-61 -0.7763572710853524 11102 62-63 -1.713714231881049 11102 64-65 -2.0318321763256435 11102 66-67 -1.951785012033529 11102 68-69 -2.1786046733811446 11102 70-71 -2.07351236247926 11102 72-73 -1.115219583082368 11102 74-75 -3.2076760598980307 11102 76 -1.5080177711664078 11103 1 -0.6719640912960578 11103 2 -3.31255117085734 11103 3 -0.04873541140519677 11103 4 -0.7217518250639721 11103 5 -0.3908359802749537 11103 6 -0.6023540910085359 11103 7 1.3651373988222133 11103 8 -1.1256126266614999 11103 9 -0.6223333344264859 11103 10-11 -1.005604105193477 11103 12-13 -0.3716215706126462 11103 14-15 -0.29973456584490066 11103 16-17 -0.7765679411512991 11103 18-19 -1.0621074067874545 11103 20-21 -0.47754759625554044 11103 22-23 0.0060015240632864675 11103 24-25 -0.7699889605697621 11103 26-27 0.1681690584287594 11103 28-29 -0.3109016508280291 11103 30-31 -0.7811605151238155 11103 32-33 -1.6694211255261173 11103 34-35 -2.0641367759841245 11103 36-37 -0.8182663229648028 11103 38-39 -1.8096142143166247 11103 40-41 -0.3987265461259497 11103 42-43 -0.11922021921824921 11103 44-45 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5 -0.9314191166027719 11105 6 0.9597026465801193 11105 7 0.15276545240771355 11105 8 -2.992633903257243 11105 9 0.1453971620274146 11105 10-11 -0.9765457915606888 11105 12-13 -0.940474998508634 11105 14-15 -1.2136431552854035 11105 16-17 -0.9768240483222996 11105 18-19 0.1844449966798507 11105 20-21 -0.9102702124887898 11105 22-23 -1.1341679006806018 11105 24-25 -0.518018905408212 11105 26-27 -0.8101603347942472 11105 28-29 0.006277230180636195 11105 30-31 -0.7531857318298805 11105 32-33 -0.31963783159388726 11105 34-35 -0.8982581313820717 11105 36-37 -0.7232111614202736 11105 38-39 -1.0548860819289168 11105 40-41 -0.8810945524301239 11105 42-43 -0.5559814485326697 11105 44-45 -0.6445521011057345 11105 46-47 0.07955914443218859 11105 48-49 -0.5863413177918027 11105 50-51 -0.42191358247308486 11105 52-53 -0.46491049575069 11105 54-55 -0.5240767341070693 11105 56-57 -0.7807241675134051 11105 58-59 -0.05088169530198172 11105 60-61 0.35451445429856676 11105 62-63 -0.8386013140852029 11105 64-65 -1.1115521401919466 11105 66-67 -2.7983297274806844 11105 68-69 0.2022670520027745 11105 70-71 -1.3104138981612827 11105 72-73 -1.2696911277978131 11105 74-75 -0.5181999984707488 11105 76 -1.1191288822775185 11106 1 0.5407077219217946 11106 2 -1.4972621480744337 11106 3 0.005303376017135264 11106 4 0.23465939692811943 11106 5 -0.8044115780908072 11106 6 -0.03955989029304163 11106 7 -0.57404542927042 11106 8 0.027277601167533305 11106 9 -0.4172872037542916 11106 10-11 -1.5346005277127688 11106 12-13 -0.047530062422097785 11106 14-15 -0.6346526603657026 11106 16-17 -1.28758281593948 11106 18-19 -0.8033049213798051 11106 20-21 -0.9623433032787005 11106 22-23 0.0880952917153337 11106 24-25 0.15766119947542734 11106 26-27 0.05152435874885697 11106 28-29 -0.34860641452601016 11106 30-31 -0.8965813398272573 11106 32-33 -1.384493616582418 11106 34-35 -1.0095331231880387 11106 36-37 -1.077398313173802 11106 38-39 -1.4204219718010904 11106 40-41 -0.7510142508896465 11106 42-43 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11108 4 -0.5064361929204892 11108 5 0.07944712153619449 11108 6 0.22760898266827212 11108 7 0.9582444851594758 11108 8 -0.1723859693729466 11108 9 -0.3332529757136271 11108 10-11 0.42959898958408615 11108 12-13 -0.32603514543241374 11108 14-15 -0.04085466431509133 11108 16-17 -0.21898046093325974 11108 18-19 0.8472472617548377 11108 20-21 1.4889109935106006 11108 22-23 -0.7101933062514689 11108 24-25 -0.2509543201618101 11108 26-27 -0.39311105410860137 11108 28-29 0.3528490085706011 11108 30-31 0.7581396431318552 11108 32-33 1.3228858904752911 11108 34-35 0.9480840785965015 11108 36-37 0.13992780399141935 11108 38-39 0.5798705755550131 11108 40-41 0.6868194359384105 11108 42-43 0.08910578746365871 11108 44-45 -0.5821860160123755 11108 46-47 -0.1178314279583823 11108 48-49 0.2740810880851505 11108 50-51 -0.4191205001345466 11108 52-53 0.5949685982193138 11108 54-55 0.28197621967315456 11108 56-57 -0.0327520218139874 11108 58-59 0.6091657488363715 11108 60-61 0.1096032207509694 11108 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1.0995219729683399 21610 46-47 1.369744329617376 21610 48-49 1.0747697933193088 21610 50-51 0.3971604916009923 21610 52-53 1.3260154301752358 21610 54-55 0.42610845107811457 21610 56-57 1.4116832398940033 21610 58-59 1.0059701565498713 21610 60-61 0.961366306150417 21610 62-63 1.2754727599888724 21610 64-65 1.372151563511757 21610 66-67 1.2600036058526491 21610 68-69 1.7691189038546256 21610 70-71 1.4055120277646402 21610 72-73 -0.7896911277978163 21610 74-75 0.7847629644922129 21610 76 0.2919822288335929 21611 1 0.8188205678718532 21611 2 1.3767021154958279 21611 3 0.6870011382627794 21611 4 0.8225631227609362 21611 5 -0.3413035233024644 21611 6 0.4209553005079272 21611 7 1.179068825844844 21611 8 1.1108692814561323 21611 9 0.8401954635136164 21611 10-11 1.152818445287629 21611 12-13 0.6190614487666721 21611 14-15 0.586191711428441 21611 16-17 0.4044864022556638 21611 18-19 1.588048440888386 21611 20-21 0.7721136044491672 21611 22-23 1.2838847060662744 21611 24-25 1.1166826751532426 21611 26-27 0.5728309367320605 21611 28-29 0.6215756496191887 21611 30-31 0.6876021898497626 21611 32-33 1.0152583703405327 21611 34-35 1.2200480729113892 21611 36-37 0.5619681261475478 21611 38-39 0.6254040808453212 21611 40-41 0.3103126190725831 21611 42-43 1.5335443830313764 21611 44-45 1.2134922489768343 21611 46-47 0.21193116613542173 21611 48-49 0.41436639629170813 21611 50-51 1.2341668610277416 21611 52-53 0.7234888908971051 21611 54-55 0.19375176318002474 21611 56-57 0.8541248534821122 21611 58-59 0.5193884155732249 21611 60-61 1.6763769218616744 21611 62-63 1.5884451591396171 21611 64-65 1.1356759796476403 21611 66-67 1.1191331175299304 21611 68-69 2.186549901731482 21611 70-71 2.0486330468729186 21611 72-73 0.9622621630726726 21611 74-75 2.0020878052565436 21611 76 0.3645936938017442 21612 1 0.9089940245877912 21612 2 0.6716586511686202 21612 3 -0.46650605237936915 21612 4 0.8141106130465587 21612 5 -0.2245707588110193 21612 6 0.7285705711084205 21612 7 0.5570282057270717 21612 8 1.3202591785037612 21612 9 0.316780809826156 21612 10-11 1.0044989324085591 21612 12-13 0.7495145193110773 21612 14-15 0.04072441983689146 21612 16-17 1.0251850362339496 21612 18-19 0.06066791771409186 21612 20-21 0.33300372182287674 21612 22-23 0.49589848646125745 21612 24-25 0.07247725112567593 21612 26-27 0.6444168839339142 21612 28-29 -0.11723779427777714 21612 30-31 0.8928659802595718 21612 32-33 1.1699673396150558 21612 34-35 0.8529647896521695 21612 36-37 1.0897692718431742 21612 38-39 0.6406065805448762 21612 40-41 0.901487557982172 21612 42-43 0.6347942328090497 21612 44-45 0.5191446144777743 21612 46-47 1.3818826943972482 21612 48-49 1.8958389757092498 21612 50-51 1.0365944538651455 21612 52-53 2.046707254074608 21612 54-55 1.2074920988768554 21612 56-57 0.11489078706381406 21612 58-59 0.9385487729020703 21612 60-61 0.5829386331944448 21612 62-63 1.381699175083213 21612 64-65 1.3688811232601878 21612 66-67 0.6395633542803232 21612 68-69 1.021886199452112 21612 70-71 1.1532478768212435 21612 72-73 1.2821956646550134 21612 74-75 1.2497944110330934 21612 76 -0.7281435573299291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 7.0 17 15.0 18 46.0 19 120.0 20 277.0 21 644.0 22 1145.0 23 1796.0 24 2681.0 25 3535.0 26 4712.0 27 6307.0 28 8146.0 29 10811.0 30 14379.0 31 19676.0 32 26216.0 33 30916.0 34 27326.0 35 12617.0 36 4777.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36669524096078 39.473400359922564 13.058263174925774 4.1016412241908835 2 25.05549405319481 25.61639558318431 33.13349797042209 16.194612393198785 3 24.925489216515565 25.73446343720373 30.436732538930112 18.903314807350597 4 18.323020153278456 24.035197275049676 33.91768379222253 23.72409877944933 5 18.082213555415525 35.98276478702931 34.14911070615552 11.785910951399652 6 29.60401941582219 41.53850520877686 17.181299497573022 11.676175877827925 7 29.2930497130303 28.384738094021607 20.118534666280635 22.203677526667462 8 26.203654818876988 40.189382972563315 16.7890048878519 16.8179573207078 9 26.359203183632225 18.610169683964326 18.125926052080317 36.904701080323136 10-11 26.78590182826617 27.529867925438754 26.60225205151277 19.081978194782305 12-13 26.591106493860384 21.645633575738994 26.75744105909135 25.005818871309277 14-15 25.294775505106415 22.452611679752028 20.318363222462548 31.93424959267901 16-17 29.37650084302672 28.882322552809804 21.17586616028294 20.565310443880534 18-19 28.86675484958757 25.187199473173276 22.559026733049862 23.387018944189293 20-21 24.718281474416838 24.80400338346078 21.960988015963576 28.51672712615881 22-23 25.237581606585298 28.91314220834516 22.12517740562021 23.72409877944933 24-25 29.12166362467392 28.804880056544164 22.40988273898567 19.66357357979625 26-27 28.891664774017716 29.143481717011127 22.797240517828754 19.167612991142402 28-29 29.130364427638245 24.501278917590014 23.689925649607808 22.678431005163933 30-31 29.17117316596124 24.062009430293326 23.466157964510074 23.300659439235357 32-33 24.48370128340151 25.298991957804734 31.383865079612903 18.833441679180847 34-35 24.480538650195292 32.877929421382504 24.60855890634935 18.03297302207285 36-37 24.175281434678205 33.52493031546798 23.222973471623778 19.07681477823004 38-39 23.82813608778832 25.14660717225562 27.896520598804436 23.128736141151627 40-41 23.70932397756432 23.41014374276177 28.9693893771147 23.911142902559213 42-43 28.21235551922247 22.5094806639871 29.107909258123847 20.170254558666574 44-45 23.35780097757038 26.608421184097736 29.53687461325794 20.496903225073943 46-47 27.391610416300022 22.105116749078892 26.141518163806367 24.36175467081472 48-49 26.53007930695824 21.459713541223156 26.501126874102333 25.50908027771628 50-51 22.145204966193777 22.303024109996535 30.56979523249939 24.981975691310296 52-53 20.777060590061936 22.746109871644215 35.157052755874226 21.319776782419627 54-55 20.21822558303245 22.936394395621864 31.37815927515498 25.467220746190705 56-57 24.34275138943293 23.308411533286783 26.30101447053948 26.047822606740805 58-59 20.268356169438505 23.700726384004223 33.28961971994902 22.741297726608252 60-61 24.547973045852707 28.486639303779143 24.979137217500895 21.986250432867255 62-63 20.07953471341105 33.943042699525115 23.181445306401667 22.795977280662164 64-65 20.1401130328397 34.78555836576428 21.69906922480818 23.375259376587838 66-67 20.217262335612688 35.00608868186424 21.034763676825825 23.741885305697245 68-69 21.436438055986056 34.955237268025726 20.301048532225195 23.307276143763023 70-71 25.692161838422717 30.471016343932195 19.94084620581206 23.895975611833027 72-73 32.61149715012376 23.733735268070035 19.413504552989533 24.241263028816675 74-75 33.69099321581652 23.349513185159953 19.254875244826707 23.70461835419683 76 33.8476823071901 23.69525107156036 19.43001504442363 23.02705157682591 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.5 8 1.0 9 3.5 10 8.0 11 12.0 12 14.0 13 13.5 14 18.5 15 32.0 16 51.0 17 62.0 18 93.0 19 138.0 20 175.5 21 199.0 22 252.5 23 396.0 24 592.5 25 699.0 26 813.5 27 973.5 28 1231.0 29 1443.0 30 1643.0 31 2189.5 32 2853.0 33 3170.0 34 3536.0 35 4315.5 36 5089.5 37 5450.0 38 6090.0 39 7270.5 40 8240.5 41 9017.5 42 9365.0 43 9734.5 44 11818.0 45 16534.5 46 19537.0 47 15280.0 48 10030.0 49 8732.0 50 8427.0 51 7553.0 52 6082.5 53 5052.0 54 4618.0 55 4258.5 56 3290.0 57 2403.0 58 2125.0 59 1933.5 60 1551.5 61 1209.5 62 1058.0 63 1021.5 64 918.5 65 776.0 66 639.5 67 579.0 68 552.5 69 497.5 70 412.5 71 356.0 72 339.0 73 319.5 74 297.5 75 278.0 76 277.0 77 267.0 78 227.0 79 196.0 80 176.0 81 158.5 82 131.5 83 102.0 84 90.5 85 70.0 86 57.0 87 53.0 88 36.5 89 20.0 90 16.5 91 14.5 92 16.0 93 14.0 94 13.0 95 15.0 96 16.0 97 23.5 98 86.5 99 4120.0 100 8098.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0011353895237608644 2 0.0034061685712825926 3 0.0011353895237608644 4 5.676947618804322E-4 5 0.0 6 0.0034061685712825926 7 0.0 8 0.0 9 0.0 10-11 2.838473809402161E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0011353895237608644 20-21 0.0 22-23 5.676947618804322E-4 24-25 0.0031223211903423764 26-27 0.017598537618293397 28-29 0.014476216427951018 30-31 0.00936696357102713 32-33 0.009934658332907563 34-35 0.003973863333163025 36-37 0.0 38-39 0.0 40-41 0.0017030842856412963 42-43 0.0017030842856412963 44-45 0.0 46-47 0.002270779047521729 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0017030842856412963 56-57 0.0 58-59 0.0025546264284619447 60-61 0.0 62-63 8.515421428206482E-4 64-65 0.0042577107141032414 66-67 0.0025546264284619447 68-69 0.0 70-71 0.0 72-73 0.0017030842856412963 74-75 0.0034061685712825926 76 0.0034061685712825926 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 176151.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.79931990167527 #Duplication Level Percentage of deduplicated Percentage of total 1 73.31228198171496 36.509017831292475 2 11.950251932240487 11.90228837758514 3 6.012174825015389 8.982066522472197 4 3.2568796881056064 6.487615738769578 5 1.7509860696290556 4.359895771241719 6 0.9518706823829826 2.844150757020965 7 0.6315405485511046 2.2015202865723156 8 0.4274868334055311 1.7030842856412962 9 0.35110918583707623 1.573649879932558 >10 1.3212193064453615 10.01300021004706 >50 0.01823943822530266 0.6715829033045512 >100 0.00911971911265133 0.7658202337767029 >500 0.003419894667244249 1.2670947085171245 >1k 0.0011399648890814162 0.585860994260606 >5k 0.0022799297781628323 10.133351499565714 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT 9691 5.501529937383268 RNA PCR Primer, Index 19 (95% over 21bp) GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 8159 4.631821562182446 No Hit CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT 1032 0.585860994260606 No Hit ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT 908 0.5154668437874323 No Hit TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG 809 0.45926506236126957 No Hit ATCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC 515 0.29236280236842255 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC 285 0.16179300713592315 No Hit ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGACGT 248 0.14078830094634717 No Hit ATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC 182 0.10332044666223864 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.676947618804321E-4 0.0 5 0.0 0.0 0.0 0.0011353895237608642 0.0 6 0.0 0.0 0.0 0.0011353895237608642 0.0 7 0.0 0.0 0.0 0.0011353895237608642 0.0 8 0.0 0.0 0.0 0.0017030842856412963 0.0 9 0.0 0.0 0.0 0.005109252856923889 0.0 10 0.0 0.0 0.0 0.006812337142565185 0.0 11 0.0 0.0 0.0 0.009083116190086914 0.0 12 0.0 0.0 0.0 0.009083116190086914 0.0 13 0.0 0.0 0.0 0.011353895237608642 0.0 14 0.0 0.0 0.0 0.011353895237608642 0.0 15 0.0 0.0 0.0 0.011921589999489075 0.0 16 0.0 0.0 0.0 0.014192369047010803 0.0 17 0.0 0.0 0.0 0.017030842856412962 0.0 18 0.0 0.0 0.0 0.017030842856412962 0.0 19 0.0 0.0 0.0 0.022707790475217284 0.0 20 0.0 0.0 0.0 0.02611395904649988 0.0 21 0.0 0.0 0.0 0.034061685712825925 0.0 22 0.0 0.0 0.0 0.038603243807869385 0.0 23 0.0 0.0 0.0 0.04030632809351068 0.0 24 0.0 0.0 0.0 0.047118665236075864 0.0 25 0.0 0.0 0.0 0.052795612854880186 0.0 26 0.0 0.0 0.0 0.05847256047368451 0.0 27 0.0 0.0 0.0 0.07720648761573877 0.0 28 0.0 0.0 0.0 0.10559122570976037 0.0 29 0.0 0.0 0.0 0.14249138523198845 0.0 30 0.0 0.0 0.0 0.21288553570516205 0.0 31 0.0 0.0 0.0 0.26454575903628136 0.0 32 0.0 0.0 0.0 0.3406168571282593 0.0 33 0.0 0.0 0.0 0.42293259760092194 0.0 34 0.0 0.0 0.0 0.5415808028339323 0.0 35 0.0 0.0 0.0 0.6903168304466055 0.0 36 0.0 0.0 0.0 0.9395348309121152 0.0 37 0.0 0.0 0.0 1.3942583351783413 0.0 38 0.0 0.0 0.0 2.0499457851502405 0.0 39 0.0 0.0 0.0 2.789084365118563 0.0 40 0.0 0.0 0.0 3.7121560479361455 0.0 41 0.0 0.0 0.0 4.7396835669397275 0.0 42 0.0 0.0 0.0 5.876776174986233 0.0 43 0.0 0.0 0.0 6.972994760177348 0.0 44 0.0 0.0 0.0 8.138472106317876 0.0 45 0.0 0.0 0.0 9.356745065313282 0.0 46 0.0 0.0 0.0 10.689124671446656 0.0 47 0.0 0.0 0.0 11.935782368536085 0.0 48 0.0 0.0 0.0 13.134186010865678 0.0 49 0.0 0.0 0.0 14.177608983201912 0.0 50 0.0 0.0 0.0 15.236359714108918 0.0 51 0.0 0.0 0.0 16.30305817168225 0.0 52 0.0 0.0 0.0 17.371459713541224 0.0 53 0.0 0.0 0.0 18.36662863111762 0.0 54 0.0 0.0 0.0 19.34079284250444 0.0 55 0.0 0.0 0.0 20.28259845246408 0.0 56 0.0 0.0 0.0 21.146629880046095 0.0 57 0.0 0.0 0.0 21.936293293821777 0.0 58 0.0 0.0 0.0 22.71233203331233 0.0 59 0.0 0.0 0.0 23.485532298993476 0.0 60 0.0 0.0 0.0 24.235457079437527 0.0 61 0.0 0.0 0.0 24.97175718559645 0.0 62 0.0 0.0 0.0 25.66775096366186 0.0 63 0.0 0.0 0.0 26.33763078268077 0.0 64 0.0 0.0 0.0 27.003536738366517 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAACG 15 0.0022226635 69.992905 33 ATACCGT 15 0.0022226635 69.992905 1 AACACCG 15 0.0022226635 69.992905 10 ATTATAC 1500 0.0 60.89382 1 AGGGGGG 1405 0.0 57.804142 70 GGTGTAA 25 2.3866595E-4 55.99432 1 CGCGGTC 20 0.006937307 52.509583 44 CTTTTGT 20 0.006937307 52.509583 46 GCTATAC 20 0.0069451025 52.494675 3 ACTTCGA 20 0.0069451025 52.494675 30 AATTGCT 20 0.0069451025 52.494675 18 CGTCTTC 1770 0.0 52.000374 48 CCGTCTT 1785 0.0 51.955513 47 TATGCCG 1880 0.0 51.950966 43 GCCGTCT 1815 0.0 51.882748 46 CGTATGC 1910 0.0 51.684826 41 TCGTATG 1945 0.0 51.474686 40 ATGCCGT 1880 0.0 51.392353 44 CTTGAAA 1860 0.0 51.365765 57 ATCTCGT 1910 0.0 51.318268 37 >>END_MODULE