FastQCFastQC Report
Wed 25 May 2016
SRR1779877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373550
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3968110.622674340784366No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG80722.16088876991032No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC12040.32231294338107347No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG9640.25806451612903225No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT9000.24093160219515458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG150.00222372670.010
TCGCCGT13700.048.7956252
CGTATCA13700.048.7956256
GCCGTAT13650.048.7179554
TCGGTGG13950.048.42293545
CGGTGGT14000.048.2546
CTCGGTG13950.048.17204344
GTCGCCG14050.048.0782951
TGGTCGC13950.047.92114649
CGCCGTA14100.047.16312453
TCTCGGT14250.047.15789443
GTATCAT14150.046.99646857
TAGATCT14600.046.74657439
AGATCTC14400.046.66666440
GTACTAG305.874281E-446.6666641
GATCTCG14450.046.50519641
CCGTATC14350.046.34146555
GTAGATC14750.046.27118738
ATCTCGG14550.046.18556642
GGTGGTC14900.045.80536747
Produced by FastQC (version 0.11.5)