FastQCFastQC Report
Wed 25 May 2016
SRR1779877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373550
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT143243.834560299825994No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140713.7668317494311334No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT120583.227948065854638No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG24040.6435550796412796No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT13310.35631106946861196No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC11840.31695890777673674No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC8120.2173738455360728No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC6140.1643688930531388No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTT150.002221820270.0151330
TTATCCG150.002221820270.0151322
ATTATAC20450.061.781651
AGGGGGG33900.058.64004570
GTTCGAA252.3895505E-455.99711238
TGTTCGA252.3895505E-455.99711237
ACAGGTA200.00694612252.5043220
AATCACG200.006949852.4972936
CCGTGCG200.006949852.497299
CATCTCG200.006949852.4972936
ACTTACG200.006949852.497291
TTACACG200.006949852.497299
CGTATGC44100.051.34655441
CGTCTTC42200.051.3362148
TATGCCG43400.051.2070343
CCGTCTT41950.051.0581547
CTCGTAT44350.051.05711439
TCGTATG44900.051.05526740
GCCGTCT42400.051.01151746
CTTGAAA42650.050.79456357
Produced by FastQC (version 0.11.5)