FastQCFastQC Report
Wed 25 May 2016
SRR1779876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151607
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3210521.176462828233525No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG64064.225398563390873No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC10350.6826861556524435No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG7510.49535971294201453No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT6960.4590817046706287No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG2270.1497292341382654No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG1810.119387627220379No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC150.002221170870.050
TGGTCGA150.002221170870.049
ATCCTGA200.006940460352.51
TAGAGCG200.006940460352.522
AACCGGC200.006940460352.523
GTCGACG200.006940460352.551
TGCCAGC200.006940460352.522
TTAGCGC200.006940460352.559
GTCCTGG200.006940460352.51
CGCATTT200.006940460352.543
AGTGCGA200.006940460352.550
TAACCGG200.006940460352.522
TGCGCGT200.006940460352.55
GACGGGT200.006940460352.516
GGGTTAC200.006940460352.51
CTTAGCG200.006940460352.558
CGAATCA352.078829E-550.056
TCTTTAT352.078829E-550.01
GTGCTAG305.863179E-446.66666464
AACCTCG305.863179E-446.66666419
Produced by FastQC (version 0.11.5)