FastQCFastQC Report
Wed 25 May 2016
SRR1779876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151607
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT136869.027287658221587No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110677.299794864353229No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT85145.615835680410535No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG23571.5546775544664824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC15291.0085286299445277No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC8260.5448297242211771No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT6420.4234632965496316TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC3860.25460565805009006No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2020.13323923037854454No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT1740.11477042616765717No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCT150.00221888770.0181525
CCCTTGA207.909239E-569.99505612
CCTTATA150.002221793269.995051
AAGCATG150.002221793269.9950554
CGAATGC150.002221793269.9950541
CCTTGAT150.002221793269.9950513
ATTATAC12300.065.442531
AGGGGGG25500.063.13279370
ATGGGTC308.332376E-658.34845730
CTTAAGC308.348696E-658.3292151
CGTCTTA850.057.64298248
TATGACG800.056.8709843
AACCGAT252.3813454E-456.01451528
ACCGATA252.3813454E-456.01451529
TCTGCGG252.3852322E-455.99604417
ATCAGCT252.3852322E-455.99604452
AGTCTTC252.3852322E-455.99604448
AGCTAGA252.3852322E-455.99604455
TGACGTC700.054.9961145
CACGTAT452.6842827E-854.44059839
Produced by FastQC (version 0.11.5)