FastQCFastQC Report
Wed 25 May 2016
SRR1779875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207288
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3983719.218189186059973No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG78133.769152097564741No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC11670.5629848326965382No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG8990.4336961136196982No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT8690.4192234958125892No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG2460.11867546601829339No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG2280.10999189533402803No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGTC150.002222537569.99831412
GAACGAG150.002222537569.99831469
CGGTAGA200.006938076652.51140214
GCGACCG200.00694470252.49873429
GTGGAAC200.00694470252.49873466
GTAGCGA200.00694470252.49873422
GTTGTAC200.00694470252.4987348
GGAACGA200.00694470252.49873468
TAAATTG200.00694470252.4987345
GCGCGTC352.0817986E-549.998796
TCGGTGG14200.045.84396445
CGGTGGT14000.045.74889446
GCCGTAT13800.045.6510754
TGGTCGC13900.045.5744449
ATCTCGG14100.045.4244442
CTCGGTG14200.045.35101744
TCTCGGT14300.045.2786343
GATCTCG14150.045.26392441
TCGCCGT13850.045.23356652
CGCCGTA13850.045.23356653