FastQCFastQC Report
Wed 25 May 2016
SRR1779875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207288
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT148527.164910655706071No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134986.511713172011886No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT132136.374223302844352No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG26171.2624946933734706No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC12860.6203928833314036No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT11150.5378989618308827RNA PCR Primer, Index 19 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC10230.4935162672224152No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC5800.27980394427077304No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC2600.12542935432827756No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT2360.11385126008259042No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTT150.002221261670.00844624
AAACTCG207.9180456E-569.9915636
ATCGTAT455.456968E-1269.9915639
ATTATAC17300.064.9500351
AGGGGGG30950.061.865370
ATATCGT401.0624717E-861.24261537
AATCACG308.361958E-658.32629436
GCGAACT252.3850272E-456.00675628
CTGAGCG252.3850272E-456.00675626
GAGCGTC252.3850272E-456.00675628
CACGTAT252.3878735E-455.99324439
CGTATGA700.054.99336641
CGTCTTC38550.053.7548648
TCTTCTG38200.053.60594650
CCGTCTT38350.053.565947
TATGCCG39700.053.50739743
GCCGTCT38650.053.42175746
GTCTTCT38400.053.417949
ATGCCGT39400.053.29306444
TGCCGTC39400.053.29306445
Produced by FastQC (version 0.11.5)