FastQCFastQC Report
Wed 25 May 2016
SRR1779874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148966
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT150.00222109470.035
GCCGTAA150.00222109470.054
GGTATAA252.3842632E-455.9999961
ACGAAGT252.3842632E-455.99999624
CGGTGGT1400.052.50000446
TGGTCGC1400.052.50000449
GGTCGCC1600.052.550
GGACTCG200.006940223352.514
ATATCGG200.006940223352.542
TCTCGTA200.006940223352.547
GCTTAAG200.006940223352.51
TCGTCCC200.006940223352.549
GTTCCGC200.006940223352.58
GCGTGGT200.006940223352.57
GATCTCG1600.052.541
GTTGGGT200.006940223352.51
TAGTGTA750.051.33333234
TCGGTGG1450.050.68965545
CGTGTAG352.0786518E-550.00000435
GTGGTCG1400.050.00000448
Produced by FastQC (version 0.11.5)