##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779874_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	148966
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.74457929997449	34.0	23.0	34.0	12.0	34.0
2	28.265375991837065	34.0	23.0	34.0	12.0	34.0
3	28.03807580253212	34.0	23.0	34.0	11.0	34.0
4	28.355201858142127	34.0	23.0	34.0	12.0	34.0
5	28.24211565055113	34.0	23.0	34.0	11.0	34.0
6	31.135554421814373	37.0	26.0	38.0	12.0	38.0
7	31.294241639031725	37.0	26.0	38.0	12.0	38.0
8	30.705469704496327	37.0	26.0	38.0	11.0	38.0
9	31.398473477169286	37.0	27.0	38.0	11.0	38.0
10-11	30.11295530523744	36.5	24.5	38.0	11.0	38.0
12-13	30.788065733120312	37.0	26.0	38.0	11.0	38.0
14-15	29.76713813890418	36.0	22.5	38.0	11.0	38.0
16-17	30.218006122202382	36.5	24.0	38.0	11.0	38.0
18-19	30.849079655760374	37.0	26.0	38.0	11.0	38.0
20-21	29.914913470187827	36.0	23.5	38.0	11.0	38.0
22-23	30.59665292751366	37.0	24.5	38.0	11.0	38.0
24-25	30.927553938482607	37.0	26.0	38.0	11.0	38.0
26-27	29.938251010297652	37.0	22.5	38.0	11.0	38.0
28-29	30.890203133600956	37.0	26.0	38.0	11.0	38.0
30-31	31.145147214800694	37.0	26.0	38.0	11.0	38.0
32-33	31.316377562665306	38.0	26.0	38.0	11.0	38.0
34-35	31.31958634856276	37.0	26.0	38.0	11.0	38.0
36-37	30.885155001812493	37.0	25.5	38.0	11.0	38.0
38-39	30.83229394626962	37.0	25.0	38.0	11.0	38.0
40-41	30.83545909804922	37.0	25.5	38.0	11.0	38.0
42-43	31.136400252406588	37.0	26.0	38.0	11.0	38.0
44-45	31.197420887987864	37.0	26.0	38.0	11.0	38.0
46-47	31.0936052522052	37.0	26.0	38.0	11.0	38.0
48-49	30.807207684975097	37.0	25.0	38.0	11.0	38.0
50-51	30.32106990856974	37.0	24.0	38.0	11.0	38.0
52-53	30.816233234429333	37.0	25.5	38.0	11.0	38.0
54-55	30.911708040760978	37.0	25.5	38.0	11.0	38.0
56-57	30.954499684491765	37.0	25.5	38.0	11.0	38.0
58-59	31.063145281473624	37.0	25.5	38.0	11.0	38.0
60-61	31.024270638937743	37.0	25.0	38.0	11.0	38.0
62-63	30.983254568156493	37.0	25.0	38.0	11.0	38.0
64-65	30.9716848139844	37.0	25.0	38.0	11.0	38.0
66-67	30.905397876025404	37.0	25.0	38.0	11.0	38.0
68-69	30.40926788663185	37.0	24.0	38.0	11.0	38.0
70-71	30.493206503497444	37.0	24.0	38.0	11.0	38.0
72-73	30.529651061316006	37.0	24.0	38.0	11.0	38.0
74-75	30.598804425170847	37.0	24.0	38.0	11.0	38.0
76	29.897654498341904	36.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	77.0
13	887.0
14	2587.0
15	4015.0
16	5254.0
17	6042.0
18	6094.0
19	5217.0
20	3926.0
21	3022.0
22	2272.0
23	1724.0
24	1467.0
25	1420.0
26	1545.0
27	1622.0
28	1792.0
29	2161.0
30	2605.0
31	3125.0
32	4201.0
33	5213.0
34	7417.0
35	11069.0
36	20123.0
37	44086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.48123732932347	21.79557751433213	14.552985244955224	31.170199911389172
2	19.032530913094263	21.845924573392587	35.359746519339986	23.76179799417317
3	16.327886900366526	26.492622477612343	32.50204744706846	24.677443174952675
4	12.023548997757878	25.113113059355825	29.70006578682384	33.163272156062455
5	16.691728313843427	33.43850274559295	32.32214062269242	17.54762831787119
6	24.866744538875686	42.46653508948591	15.330755494689921	17.335964876948484
7	21.558570594198326	33.80505783548272	18.983330759883724	25.653040810435222
8	18.70544165626133	43.65341496489037	17.153367973040105	20.487775405808193
9	21.329546904056983	19.900236987506126	18.870381932555908	39.89983417588098
10-11	21.128207193780966	29.819685557390475	23.617096977752716	25.435010271075846
12-13	20.731576332854477	25.057395647328924	25.948538592699006	28.2624894271176
14-15	24.004631960527643	27.5829892927869	21.676232672104184	26.736146074581278
16-17	24.70748162320008	27.581982344846107	21.974960561205652	25.735575470748163
18-19	20.384660568935136	27.52806914491902	25.25266426113955	26.83460602500629
20-21	24.204852113905186	26.429185183196164	23.41138246311239	25.954580239786264
22-23	24.439961601202985	26.321972570369823	22.96711263870894	26.270953189718256
24-25	20.422398109546922	29.90789411851583	22.580710128290335	27.088997643646913
26-27	23.269739403622303	29.584267550984787	23.20764469744774	23.93834834794517
28-29	20.855417521222638	29.34044502925351	24.667604300584397	25.136533148939456
30-31	21.80661099326012	28.451169410058807	24.642866732901908	25.099352863779167
32-33	19.55721291171446	29.346056637275076	25.483081653134448	25.61364879787602
34-35	19.85190704918418	30.512451287421833	24.494077288945725	25.14156437444826
36-37	22.492312443770057	27.400263189698006	25.056062091283852	25.051362275248085
38-39	22.79224179601482	25.860344271980235	26.600851280949566	24.746562651055374
40-41	20.63303460544423	28.5969858691639	25.55499614003289	25.214983385358973
42-43	18.517101332527773	27.917967307756857	26.63343738462055	26.931493975094824
44-45	18.627671465542416	25.95219473880309	28.009814814193213	27.41031898146128
46-47	19.66125247968421	27.16726917538542	26.919216839363457	26.25226150556691
48-49	20.04826620929007	27.22442444727135	26.673625772410393	26.05368357102819
50-51	23.231763805534296	27.21700836454935	24.425356802405982	25.12587102751037
52-53	17.17321248741188	29.07821416582746	26.221886539107082	27.526686807653576
54-55	18.68475290863561	26.893046799997318	26.006525548327325	28.415674743039748
56-57	19.545616284768613	28.09552262855071	24.65038368837656	27.708477398304115
58-59	18.50326297131808	28.258607261789663	25.744575142335375	27.493554624556882
60-61	17.703696243327627	27.141706113405178	25.881089065699804	29.273508577567398
62-63	19.089834912152316	27.652382327074005	24.99916079784627	28.258621962927403
64-65	19.06411663796374	26.817936141856258	24.791274309367225	29.32667291081278
66-67	18.429728205403663	30.1921630767578	22.938040500617713	28.44006821722082
68-69	19.03897211099214	31.230049910885405	22.43472481883389	27.296253159288558
70-71	19.37394900161447	31.398209652570564	21.916148369924578	27.311692975890388
72-73	20.6704416913095	29.712375345307347	22.175528576175722	27.441654387207432
74-75	19.756028787797412	28.929453783769265	23.61062624201085	27.703891186422467
76	19.206240517715926	31.00119493562116	21.974731810797383	27.817832735865522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	3.5
10	3.5
11	1.0
12	2.0
13	3.0
14	3.0
15	13.0
16	32.0
17	40.0
18	59.5
19	111.0
20	205.0
21	267.0
22	291.5
23	410.5
24	603.5
25	702.0
26	843.5
27	1081.5
28	1398.0
29	1618.0
30	1865.5
31	2463.5
32	2999.5
33	3185.0
34	3529.0
35	4109.5
36	4622.5
37	4899.0
38	5206.5
39	5745.5
40	6202.0
41	6742.0
42	7057.0
43	7154.5
44	7427.0
45	7649.0
46	7696.0
47	7678.5
48	7542.0
49	7290.0
50	7157.0
51	6928.5
52	6395.5
53	5927.0
54	5763.0
55	5478.5
56	4903.5
57	4312.5
58	4012.0
59	3695.0
60	3072.5
61	2530.5
62	2294.0
63	2148.5
64	1820.0
65	1476.5
66	1215.5
67	1115.0
68	1001.0
69	831.5
70	720.0
71	664.0
72	623.5
73	519.0
74	398.5
75	342.0
76	328.5
77	289.0
78	227.0
79	191.0
80	181.0
81	149.0
82	117.0
83	107.0
84	92.5
85	73.5
86	58.5
87	48.0
88	42.5
89	29.0
90	19.0
91	19.5
92	22.0
93	17.5
94	9.0
95	5.5
96	6.0
97	5.0
98	5.5
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.00268517648322436
7	0.00604164708725481
8	0.00268517648322436
9	0.008726823570479169
10-11	0.00268517648322436
12-13	0.0
14-15	6.7129412080609E-4
16-17	6.7129412080609E-4
18-19	0.002349529422821315
20-21	0.0
22-23	0.00201388236241827
24-25	0.00469905884564263
26-27	0.0
28-29	0.006377294147657855
30-31	0.00134258824161218
32-33	3.35647060403045E-4
34-35	0.003692117664433495
36-37	0.01611105889934616
38-39	0.00939811769128526
40-41	6.7129412080609E-4
42-43	6.7129412080609E-4
44-45	0.005034705906045675
46-47	0.003692117664433495
48-49	3.35647060403045E-4
50-51	0.00268517648322436
52-53	0.01074070593289744
54-55	0.008726823570479169
56-57	0.01141200005370353
58-59	0.01476847065773398
60-61	0.02081011774498879
62-63	0.01006941181209135
64-65	0.017789294201361383
66-67	0.0201388236241827
68-69	3.35647060403045E-4
70-71	3.35647060403045E-4
72-73	0.003020823543627405
74-75	0.00939811769128526
76	0.00268517648322436
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	148966.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.76024109280412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.85950876996641	58.02497843978498
2	8.713117649205813	12.505108463539516
3	5.100634699895783	10.98068327372532
4	2.7999402478022044	8.036975489109278
5	1.412223398532969	5.067074577781253
6	0.6581593116214638	2.833780252765814
7	0.2696549520686205	1.3545353060618923
8	0.11784917021027365	0.6765507893500917
9	0.04294503527890879	0.27735714768181263
>10	0.024968043670971555	0.20210520760727987
>50	9.98721746599884E-4	0.040851052592761436
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00537035296644872	0.0
2	0.0	0.0	0.0	0.00537035296644872	0.0
3	0.0	0.0	0.0	0.00537035296644872	0.0
4	0.0	0.0	0.0	0.01208329417450962	0.0
5	0.0	0.0	0.0	0.01208329417450962	0.0
6	0.0	0.0	0.0	0.01208329417450962	0.0
7	0.0	0.0	0.0	0.014768470657733979	0.0
8	0.0	0.0	0.0	0.014768470657733979	0.0
9	0.0	0.0	0.0	0.0201388236241827	0.0
10	0.0	0.0	0.0	0.02148141186579488	0.0
11	0.0	0.0	0.0	0.02148141186579488	0.0
12	0.0	0.0	0.0	0.02349529422821315	0.0
13	0.0	0.0	0.0	0.02550917659063142	0.0
14	0.0	0.0	0.0	0.028194353073855778	0.0
15	0.0	0.0	0.0	0.029536941315467958	0.0
16	0.0	0.0	0.0	0.030208235436274048	0.0
17	0.0	0.0	0.0	0.030208235436274048	0.0
18	0.0	0.0	0.0	0.03222211779869232	0.0
19	0.0	0.0	0.0	0.03759247076514104	0.0
20	0.0	0.0	0.0	0.04162023548997758	0.0
21	0.0	0.0	0.0	0.04497670609400803	0.0
22	0.0	0.0	0.0	0.05034705906045675	0.0
23	0.0	0.0	0.0	0.05504611790609938	0.0
24	0.0	0.0	0.0	0.061087764993354186	0.0
25	0.0	0.0	0.0	0.061087764993354186	0.0
26	0.0	0.0	0.0	0.06377294147657855	0.0
27	0.0	0.0	0.0	0.07182847092625162	0.0
28	0.0	0.0	0.0	0.08525435334237343	0.0
29	0.0	0.0	0.0	0.10472188284575004	0.0
30	0.0	0.0	0.0	0.12418941234912664	0.0
31	0.0	0.0	0.0	0.15171247130217633	0.0
32	0.0	0.0	0.0	0.18527717734248084	0.0
33	0.0	0.0	0.0	0.22488353047004014	0.0
34	0.0	0.0	0.0	0.290670354309037	0.0
35	0.0	0.0	0.0	0.402776472483654	0.0
36	0.0	0.0	0.0	0.5692574144435643	0.0
37	0.0	0.0	0.0	0.8082381214505323	0.0
38	0.0	0.0	0.0	1.1452277700951896	0.0
39	0.0	0.0	0.0	1.4828887128606527	0.0
40	0.0	0.0	0.0	1.909831773693326	0.0
41	0.0	0.0	0.0	2.4837882469825328	0.0
42	0.0	0.0	0.0	3.1074204852113905	0.0
43	0.0	0.0	0.0	3.6444557818562626	0.0
44	0.0	0.0	0.0	4.366768255843615	0.0
45	0.0	0.0	0.0	5.032020729562451	0.0
46	0.0	0.0	0.0	5.567042143844904	0.0
47	0.0	0.0	0.0	6.085281205107206	0.0
48	0.0	0.0	0.0	6.710927325698481	0.0
49	0.0	0.0	0.0	7.19023132795403	0.0
50	0.0	0.0	0.0	7.705785212733107	0.0
51	0.0	0.0	0.0	8.305922156733752	0.0
52	0.0	0.0	0.0	8.884577688868601	0.0
53	0.0	0.0	0.0	9.498140515285368	0.0
54	0.0	0.0	0.0	10.06068498852087	0.0
55	0.0	0.0	0.0	10.571539814454304	0.0
56	0.0	0.0	0.0	11.013922640065518	0.0
57	0.0	0.0	0.0	11.529476524844595	0.0
58	0.0	0.0	0.0	12.026234174241102	0.0
59	0.0	0.0	0.0	12.406857940738155	0.0
60	0.0	0.0	0.0	12.836486178054052	0.0
61	0.0	0.0	0.0	13.273498650698818	0.0
62	0.0	0.0	0.0	13.725279594001316	0.0
63	0.0	0.0	0.0	14.193842890323966	0.0
64	0.0	0.0	0.0	14.576480539183438	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGAT	15	0.002221094	70.0	35
GCCGTAA	15	0.002221094	70.0	54
GGTATAA	25	2.3842632E-4	55.999996	1
ACGAAGT	25	2.3842632E-4	55.999996	24
CGGTGGT	140	0.0	52.500004	46
TGGTCGC	140	0.0	52.500004	49
GGTCGCC	160	0.0	52.5	50
GGACTCG	20	0.0069402233	52.5	14
ATATCGG	20	0.0069402233	52.5	42
TCTCGTA	20	0.0069402233	52.5	47
GCTTAAG	20	0.0069402233	52.5	1
TCGTCCC	20	0.0069402233	52.5	49
GTTCCGC	20	0.0069402233	52.5	8
GCGTGGT	20	0.0069402233	52.5	7
GATCTCG	160	0.0	52.5	41
GTTGGGT	20	0.0069402233	52.5	1
TAGTGTA	75	0.0	51.333332	34
TCGGTGG	145	0.0	50.689655	45
CGTGTAG	35	2.0786518E-5	50.000004	35
GTGGTCG	140	0.0	50.000004	48
>>END_MODULE