FastQCFastQC Report
Wed 25 May 2016
SRR1779874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148966
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2729518.322973027402227No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG35142.3589275405126No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC18111.215713652779829No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT8020.5383778848864842RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC7470.5014567082421493No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT3170.21280023629553052No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3160.21212894217472444No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT2590.1738651772887773No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT2520.16916611844313467No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2220.14902729481895197RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC1870.12553200059073882No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1630.10942094169139266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTA207.908458E-569.99563626
GCTCGTA308.347568E-658.32969738
ATAACTA252.3849978E-455.996515
AATCTCG39000.052.8556836
TCTCGTA39850.052.78215438
CTCGTAT41300.052.70857639
ATCTCGT39850.052.69433237
TCGCCAG200.00693271852.5143661
CTTCGAC200.00694193352.49672726
CGCGGAC200.00694193352.49672723
TGTGACG200.00694193352.49672712
GCGGGCT200.00694193352.49672747
CTTTCGC200.00694193352.49672728
TTATTCG200.00694193352.49672719
TCGTATG41900.052.37143340
GTCAATC38900.052.27180533
CGTATGC41900.052.2043841
CAATCTC39300.052.1850435
CTGCTTG41550.052.13874454
TATGCCG41850.052.099543