##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779874_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	148966
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.56206785440973	34.0	34.0	34.0	34.0	34.0
2	33.68680101499671	34.0	34.0	34.0	34.0	34.0
3	33.77988937072889	34.0	34.0	34.0	34.0	34.0
4	33.58949021924466	34.0	34.0	34.0	34.0	34.0
5	33.63656807593679	34.0	34.0	34.0	34.0	34.0
6	37.33600284628707	38.0	38.0	38.0	37.0	38.0
7	37.38525569593062	38.0	38.0	38.0	37.0	38.0
8	37.26011304592994	38.0	38.0	38.0	36.0	38.0
9	37.20228777036371	38.0	38.0	38.0	36.0	38.0
10-11	37.15375656190003	38.0	38.0	38.0	36.5	38.0
12-13	37.307331874387444	38.0	38.0	38.0	36.5	38.0
14-15	37.323530201522495	38.0	38.0	38.0	37.0	38.0
16-17	37.202418672717265	38.0	38.0	38.0	36.0	38.0
18-19	37.06965012150424	38.0	38.0	38.0	35.5	38.0
20-21	37.11137105111233	38.0	38.0	38.0	36.0	38.0
22-23	37.12124578762939	38.0	38.0	38.0	36.0	38.0
24-25	37.27085039539224	38.0	38.0	38.0	36.0	38.0
26-27	37.14474443832821	38.0	38.0	38.0	36.0	38.0
28-29	36.71178993864372	38.0	37.0	38.0	34.0	38.0
30-31	37.027153847186604	38.0	38.0	38.0	36.0	38.0
32-33	36.971688170455	38.0	38.0	38.0	36.0	38.0
34-35	36.942483519729336	38.0	38.0	38.0	35.5	38.0
36-37	36.86470738289274	38.0	37.5	38.0	35.5	38.0
38-39	36.01780607655438	38.0	37.0	38.0	33.0	38.0
40-41	36.6377394841776	38.0	37.0	38.0	34.5	38.0
42-43	37.12859310178162	38.0	38.0	38.0	36.0	38.0
44-45	36.91870628197039	38.0	37.5	38.0	35.0	38.0
46-47	37.05784205791926	38.0	38.0	38.0	36.0	38.0
48-49	36.704372138608804	38.0	37.0	38.0	34.5	38.0
50-51	36.82433575446746	38.0	37.0	38.0	34.5	38.0
52-53	36.72692090812669	38.0	37.0	38.0	34.5	38.0
54-55	36.74696910704456	38.0	37.0	38.0	35.0	38.0
56-57	36.629358377079335	38.0	37.0	38.0	34.0	38.0
58-59	36.664735577245814	38.0	37.0	38.0	34.5	38.0
60-61	36.32457406388035	38.0	37.0	38.0	34.0	38.0
62-63	36.18225971026946	38.0	37.0	38.0	34.0	38.0
64-65	36.59855604634615	38.0	37.0	38.0	34.0	38.0
66-67	36.299964421411595	38.0	37.0	38.0	33.5	38.0
68-69	36.29869231905267	38.0	37.0	38.0	34.0	38.0
70-71	34.787394438999506	38.0	35.5	38.0	26.5	38.0
72-73	32.16379912194729	38.0	28.0	38.0	11.0	38.0
74-75	31.743971778795164	38.0	25.0	38.0	11.0	38.0
76	31.598599680463998	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	7.0
23	13.0
24	30.0
25	85.0
26	180.0
27	297.0
28	571.0
29	905.0
30	1502.0
31	2439.0
32	3693.0
33	6186.0
34	10521.0
35	22252.0
36	26467.0
37	73817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.588505500114163	16.64137106631029	12.681154553879628	42.08896887969591
2	12.990883825839452	15.87409207470161	50.29067035430904	20.8443537451499
3	13.893103124202838	18.60357396989917	46.678436690251466	20.824886215646522
4	10.313762872064766	33.78287662956648	33.012230978881085	22.89112951948767
5	10.387605225353436	30.251869554126444	48.36539881583717	10.995126404682948
6	22.069465515621015	49.86305599935556	16.508465018863365	11.559013466160064
7	18.909012794865944	26.926949773773885	19.210423855107877	34.9536135762523
8	17.254944081199735	50.01812494126177	16.372192312339727	16.35473866519877
9	17.883946672395044	15.541130190781788	17.079736315669347	49.49518682115382
10-11	18.625726675885772	32.2197682692695	30.51266732005961	18.64183773478512
12-13	18.391445027724448	18.445484204449336	33.1683068619685	29.994763905857713
14-15	17.124713021763355	18.56933796973806	20.469435978679698	43.83651302981889
16-17	28.541076487252127	31.28431991192621	19.115435737013815	21.059167863807847
18-19	29.051595666125156	22.985110696400522	20.371427037042007	27.59186660043231
20-21	17.711088436287476	20.222399742223057	19.894472564209284	42.17203925728018
22-23	16.96897278573634	32.196608622101685	19.644751151269418	31.189667440892556
24-25	28.622639926155912	32.014433162708734	19.757992783418647	19.604934127716707
26-27	29.549695903763272	32.97900191990119	20.690627391485307	16.780674784850234
28-29	17.24890243411248	22.7625766953533	31.233637205805355	28.754883664728865
30-31	16.49134701878281	20.15963374192769	45.277445860129156	18.07157337916035
32-33	26.82558436153216	20.152585153659224	24.164238819596417	28.857591665212194
34-35	19.024811030704996	20.132781977095444	32.6165030946659	28.225903897533666
36-37	16.32083831209806	41.83807043217915	22.113435280533814	19.727655975188966
38-39	16.258407958863096	23.562087993233355	32.418806976088504	27.760697071815045
40-41	16.45509713625928	19.917632211377093	33.91142945370084	29.715841198662783
42-43	28.18471391026781	19.49948142355109	32.79014268404429	19.525661982136803
44-45	17.241518198783616	30.81676355678477	34.26956486715089	17.672153377280722
46-47	27.657317777210906	20.029402682491305	24.453902232724246	27.859377307573542
48-49	26.59734436045809	18.890887853604177	22.634695165339743	31.87707262059799
50-51	17.650198563894605	19.288056960814263	34.02576127349449	29.035983201796643
52-53	15.061695233938256	19.42787113670321	45.33113972501553	20.17929390434301
54-55	14.91359450873458	19.431328958768496	36.22750871442867	29.42756781806825
56-57	26.499222556931336	19.253050162708927	24.529252344922774	29.718474935436962
58-59	16.00441871709466	19.714663693993767	43.59050983130977	20.690407757601808
60-61	26.37977692571129	30.703603972629416	24.525077391064944	18.39154171059435
62-63	15.6122520925454	45.35611043402061	20.17640166127792	18.855235812156067
64-65	14.559687939548358	46.168900705291534	20.01973876350746	19.251672591652653
66-67	14.463191471501721	46.50381935700496	19.511458071014857	19.521531100478466
68-69	14.497861741623197	46.215584781776805	19.617597497197103	19.6689559794029
70-71	15.159724460199806	44.263951552261254	19.839335589214738	20.7369883983242
72-73	15.876335729225065	38.69695270771575	21.161173796710656	24.26553776634852
74-75	16.70622278768266	36.1285579527945	21.70405755163836	25.461161707884482
76	16.44175897952333	36.76737160120846	21.46156428331655	25.329305135951664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	4.0
14	15.0
15	20.5
16	29.0
17	41.0
18	75.5
19	140.0
20	205.0
21	240.0
22	278.5
23	441.5
24	660.5
25	755.0
26	913.0
27	1235.5
28	1540.5
29	1681.0
30	1845.5
31	2352.5
32	2776.0
33	2857.0
34	3136.5
35	3803.5
36	4448.5
37	4706.0
38	4832.5
39	8910.0
40	15394.5
41	16446.0
42	14964.0
43	13020.5
44	9405.0
45	6921.5
46	6110.0
47	5626.0
48	5117.0
49	4760.5
50	4429.0
51	4220.0
52	3829.5
53	3480.0
54	3312.0
55	3179.5
56	2895.0
57	2513.5
58	2284.0
59	2237.5
60	1911.0
61	1560.5
62	1490.0
63	1394.5
64	1224.5
65	1026.0
66	821.5
67	741.0
68	700.5
69	601.0
70	526.0
71	510.0
72	481.5
73	402.0
74	307.5
75	264.0
76	269.5
77	239.5
78	210.0
79	216.0
80	182.5
81	134.5
82	90.0
83	60.0
84	56.0
85	46.5
86	31.5
87	22.0
88	19.0
89	9.5
90	3.5
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0402776472483654
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.002349529422821315
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.35647060403045E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014432823597330936
52-53	0.03255776485909537
54-55	0.05034705906045675
56-57	0.05403917672489024
58-59	0.036249882523528856
60-61	0.03155082367788623
62-63	0.029872588375871006
64-65	0.013761529476524845
66-67	0.03591423546312581
68-69	0.008726823570479169
70-71	0.01476847065773398
72-73	0.020474470684585747
74-75	0.014432823597330936
76	0.01074070593289744
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	148966.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.390599197132232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	41.304645744826644	12.13968288065733
2	19.15399022429309	11.25894499415974
3	14.702389109679778	12.963360766886403
4	10.323877392535746	12.136997704174107
5	6.53008085514595	9.596149456923056
6	3.7892284500479647	6.68206167850382
7	2.044219085468914	4.205657666850153
8	1.0963409620391942	2.5777694238953854
9	0.4750810835503175	1.2566625941490004
>10	0.5139098259558722	2.109877421693541
>50	0.022840436709149876	0.424929178470255
>100	0.031976611392809835	1.7104574198139173
>500	0.004568087341829976	1.0398345931286335
>1k	0.004568087341829976	3.5746411932924294
>5k	0.0	0.0
>10k+	0.002284043670914988	18.322973027402227
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	27295	18.322973027402227	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	3514	2.3589275405126	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	1811	1.215713652779829	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	802	0.5383778848864842	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	747	0.5014567082421493	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	317	0.21280023629553052	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	316	0.21212894217472444	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	259	0.1738651772887773	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT	252	0.16916611844313467	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	222	0.14902729481895197	RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC	187	0.12553200059073882	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	163	0.10942094169139266	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00604164708725481	0.0
2	0.0	0.0	0.0	0.00604164708725481	0.0
3	0.0	0.0	0.0	0.00604164708725481	0.0
4	0.0	0.0	0.0	0.014768470657733979	0.0
5	0.0	0.0	0.0	0.014768470657733979	0.0
6	0.0	0.0	0.0	0.01543976477854007	0.0
7	0.0	0.0	0.0	0.01879623538257052	0.0
8	0.0	0.0	0.0	0.01879623538257052	0.0
9	0.0	0.0	0.0	0.02618047071143751	0.0
10	0.0	0.0	0.0	0.029536941315467958	0.0
11	0.0	0.0	0.0	0.029536941315467958	0.0
12	0.0	0.0	0.0	0.03222211779869232	0.0
13	0.0	0.0	0.0	0.034236000161110586	0.0
14	0.0	0.0	0.0	0.034907294281916676	0.0
15	0.0	0.0	0.0	0.036921176644334947	0.0
16	0.0	0.0	0.0	0.03759247076514104	0.0
17	0.0	0.0	0.0	0.03759247076514104	0.0
18	0.0	0.0	0.0	0.0402776472483654	0.0
19	0.0	0.0	0.0	0.04631929433562021	0.0
20	0.0	0.0	0.0	0.05101835318126284	0.0
21	0.0	0.0	0.0	0.0537035296644872	0.0
22	0.0	0.0	0.0	0.058402588510129826	0.0
23	0.0	0.0	0.0	0.06175905911416028	0.0
24	0.0	0.0	0.0	0.06444423559738464	0.0
25	0.0	0.0	0.0	0.06444423559738464	0.0
26	0.0	0.0	0.0	0.06712941208060899	0.0
27	0.0	0.0	0.0	0.07384235328866989	0.0
28	0.0	0.0	0.0	0.08928211806720997	0.0
29	0.0	0.0	0.0	0.10874964757058658	0.0
30	0.0	0.0	0.0	0.12620329471154493	0.0
31	0.0	0.0	0.0	0.15574023602701287	0.0
32	0.0	0.0	0.0	0.19333270679215392	0.0
33	0.0	0.0	0.0	0.23696682464454977	0.0
34	0.0	0.0	0.0	0.30543882496677094	0.0
35	0.0	0.0	0.0	0.42694306083267325	0.0
36	0.0	0.0	0.0	0.6055072969670932	0.0
37	0.0	0.0	0.0	0.8619416511150195	0.0
38	0.0	0.0	0.0	1.2069868292093497	0.0
39	0.0	0.0	0.0	1.5493468308204557	0.0
40	0.0	0.0	0.0	1.9957574211565055	0.0
41	0.0	0.0	0.0	2.589181423949089	0.0
42	0.0	0.0	0.0	3.2389941328893843	0.0
43	0.0	0.0	0.0	3.804895076728918	0.0
44	0.0	0.0	0.0	4.5245223742330465	0.0
45	0.0	0.0	0.0	5.208571083334452	0.0
46	0.0	0.0	0.0	5.769772968328343	0.0
47	0.0	0.0	0.0	6.294053676677899	0.0
48	0.0	0.0	0.0	6.938496032651746	0.0
49	0.0	0.0	0.0	7.449350858585181	0.0
50	0.0	0.0	0.0	7.976988037538767	0.0
51	0.0	0.0	0.0	8.581824040385055	0.0
52	0.0	0.0	0.0	9.161822160761517	0.0
53	0.0	0.0	0.0	9.773371104815864	0.0
54	0.0	0.0	0.0	10.331216519205725	0.0
55	0.0	0.0	0.0	10.842071345139159	0.0
56	0.0	0.0	0.0	11.287810641354403	0.0
57	0.0	0.0	0.0	11.802021937891867	0.0
58	0.0	0.0	0.0	12.288710175476282	0.0
59	0.0	0.0	0.0	12.67201911845656	0.0
60	0.0	0.0	0.0	13.098962179289234	0.0
61	0.0	0.0	0.0	13.53731724017561	0.0
62	0.0	0.0	0.0	13.993797242323751	0.0
63	0.0	0.0	0.0	14.467059597492046	0.0
64	0.0	0.0	0.0	14.863123128767638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGCTA	20	7.908458E-5	69.995636	26
GCTCGTA	30	8.347568E-6	58.329697	38
ATAACTA	25	2.3849978E-4	55.99651	5
AATCTCG	3900	0.0	52.85568	36
TCTCGTA	3985	0.0	52.782154	38
CTCGTAT	4130	0.0	52.708576	39
ATCTCGT	3985	0.0	52.694332	37
TCGCCAG	20	0.006932718	52.51436	61
CTTCGAC	20	0.006941933	52.496727	26
CGCGGAC	20	0.006941933	52.496727	23
TGTGACG	20	0.006941933	52.496727	12
GCGGGCT	20	0.006941933	52.496727	47
CTTTCGC	20	0.006941933	52.496727	28
TTATTCG	20	0.006941933	52.496727	19
TCGTATG	4190	0.0	52.371433	40
GTCAATC	3890	0.0	52.271805	33
CGTATGC	4190	0.0	52.20438	41
CAATCTC	3930	0.0	52.18504	35
CTGCTTG	4155	0.0	52.138744	54
TATGCCG	4185	0.0	52.0995	43
>>END_MODULE