FastQCFastQC Report
Wed 25 May 2016
SRR1779873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55293
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTC150.002213680770.0000138
GTTTAGA353.6270649E-970.0000136
GACGTTG150.002213680770.0000114
TTCCGAC301.0063377E-770.0000119
TCGATCA150.002213680770.0000139
AAAGTTA150.002213680770.0000145
GTGTCGC150.002213680770.0000136
GTGTAGC301.0063377E-770.0000136
GTATCAC150.002213680770.0000157
TAGTGTA301.0063377E-770.0000134
TAGCTAT150.002213680770.0000139
GCGTAGC150.002213680770.0000136
ATCTCTT207.862112E-570.042
CGGCGGT207.862112E-570.046
TGGCCGC252.8053382E-670.049
TCGGCGG207.862112E-570.045
GCTCTCG207.862112E-570.041
CTAGTGT207.862112E-570.033
TCTCGGC207.862112E-570.043
TGTAGCT252.8053382E-670.037
Produced by FastQC (version 0.11.5)