##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779873_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55293
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.162769247463512	21.0	12.0	23.0	12.0	32.0
2	19.737869169695983	21.0	12.0	23.0	12.0	34.0
3	19.602155788255295	21.0	11.0	27.0	11.0	34.0
4	20.683178702548243	21.0	12.0	27.0	12.0	34.0
5	18.680465881757183	11.0	11.0	23.0	11.0	34.0
6	21.473224458792252	21.0	12.0	28.0	11.0	36.0
7	21.523538241730417	21.0	12.0	31.0	11.0	37.0
8	21.80995786085038	21.0	11.0	31.0	11.0	37.0
9	22.564067784348833	23.0	11.0	34.0	11.0	37.0
10-11	21.328251315718084	21.0	11.5	29.5	11.0	36.5
12-13	21.195259797804425	21.0	11.0	29.5	11.0	36.5
14-15	20.651357314669127	21.0	11.0	28.0	11.0	35.5
16-17	20.613694319353264	21.0	11.0	28.0	11.0	35.0
18-19	20.25299766697412	21.0	11.0	28.0	10.0	36.0
20-21	20.572947751071563	21.0	11.0	27.5	10.0	35.0
22-23	20.631544680158427	21.0	11.0	29.5	10.0	36.5
24-25	20.82118893892536	21.0	11.0	31.0	10.0	36.5
26-27	20.253241820845314	21.0	11.0	27.0	10.0	36.0
28-29	20.00468413723256	21.0	11.0	27.0	10.0	36.0
30-31	19.92676288137739	21.0	11.0	27.0	10.5	36.0
32-33	20.126345106975563	21.0	11.0	27.0	11.0	36.0
34-35	20.336597761018574	21.0	11.0	27.0	11.0	36.0
36-37	20.18039353986942	21.0	11.0	27.0	11.0	36.0
38-39	20.034877832637044	21.0	11.0	27.0	11.0	36.0
40-41	19.977139963467348	21.0	11.0	27.0	11.0	36.0
42-43	20.172770513446547	21.0	11.0	27.0	11.0	36.0
44-45	20.27444703669542	21.0	11.0	27.0	11.0	36.0
46-47	20.13157180836634	16.0	11.0	27.0	11.0	36.0
48-49	19.784457345414427	16.0	11.0	27.0	11.0	35.5
50-51	19.66370969200441	19.0	11.0	26.5	11.0	34.5
52-53	20.071464742372452	21.0	11.0	27.0	11.0	35.5
54-55	19.957155516973216	21.0	11.0	27.0	11.0	36.0
56-57	19.973025518600906	21.0	11.0	27.0	11.0	36.0
58-59	19.7289439892934	16.0	11.0	26.5	10.0	36.0
60-61	19.990523212703238	21.0	11.0	27.0	11.0	35.5
62-63	19.720000723418877	19.0	11.0	27.0	11.0	36.0
64-65	19.59876476226647	16.0	11.0	26.5	10.5	36.0
66-67	20.042437559908127	21.0	11.0	27.0	10.0	36.0
68-69	19.989926392129203	21.0	11.0	27.0	10.0	35.5
70-71	20.12837972256886	20.0	11.0	27.0	10.5	35.5
72-73	19.450699003490495	15.0	11.0	25.0	10.0	34.0
74-75	19.35263053189373	15.0	11.0	25.0	10.0	34.0
76	19.400014468377552	19.0	11.0	25.0	10.0	34.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
12	144.0
13	1096.0
14	3025.0
15	4709.0
16	6160.0
17	7056.0
18	7161.0
19	6106.0
20	4720.0
21	3424.0
22	2263.0
23	1471.0
24	1042.0
25	755.0
26	557.0
27	392.0
28	328.0
29	282.0
30	264.0
31	266.0
32	246.0
33	300.0
34	397.0
35	539.0
36	912.0
37	1678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.463205107337274	35.30645832202992	8.138462373175628	40.09187419745718
2	9.299549671748684	26.797243774076286	26.046696688550085	37.85650986562494
3	17.18300689056481	22.624925397428246	33.487059844826646	26.705007867180292
4	11.030329336444035	40.18953574593529	19.998914871683578	28.7812200459371
5	20.9592534317183	29.09771580489393	26.225742860759954	23.717287902627817
6	13.71807856471099	40.929971786153516	10.144324676264198	35.2076249728713
7	13.952647096062364	26.919529003201475	18.935735344656067	40.192088556080094
8	11.370541850538956	41.66425522679592	10.607321131447588	36.35788179121754
9	16.19969250248711	21.3457538211088	11.594465044768022	50.86008863163607
10-11	12.292806438486233	35.928923452547814	18.480806619342587	33.29746348962337
12-13	12.319823485793862	24.964281192917728	23.70281952507551	39.0130757962129
14-15	26.33178098295429	29.37468915314012	15.370981597865896	28.922548266039698
16-17	27.574919067513065	24.509431573616915	14.473803193894344	33.44184616497567
18-19	16.996590734393795	24.846040459030032	20.257548765158575	37.899820041417605
20-21	26.609154865896222	23.576221221492773	15.359991318973467	34.45463259363753
22-23	27.635850643419758	22.799576780821297	14.424720340745697	35.139852235013244
24-25	16.487610779526136	33.24470971242539	13.531379996382709	36.73629951166576
26-27	24.433472591467275	33.18684101061617	14.982004955419312	27.39768144249724
28-29	18.149755832881173	31.725447639717853	18.886778802676794	31.23801772472418
30-31	20.50152827765821	28.101318478595072	19.69126982691577	31.70588341683095
32-33	16.2696905575751	30.905358725335937	20.62828929520916	32.1966614218798
34-35	15.189907759088442	35.62940857297884	18.565744257551096	30.614939410381623
36-37	22.899030460892845	25.475725345488748	20.202952029520297	31.42229216409811
38-39	23.62128529310688	22.30452004992132	23.901640530323583	30.172554126648215
40-41	17.808764219702315	30.950572405186914	20.225887544535475	31.014775830575296
42-43	11.787312926707962	29.891938327983	22.76438938373197	35.556359361577066
44-45	12.031217500610424	25.085233181707196	26.90655549426202	35.97699382342036
46-47	16.23048959143441	28.231538586750105	22.158217431408367	33.379754390407115
48-49	17.910947136165518	29.267719241133598	20.47908415170094	32.342249470999946
50-51	27.180980457763994	27.542706251526035	15.087582857814633	30.188730432895344
52-53	10.752270014108454	33.560937669572766	19.600803096624826	36.08598921969396
54-55	15.220654729607524	27.258093687827817	18.756556339301863	38.7646952432628
56-57	17.975780267882175	30.186034312794497	16.22306029609934	35.61512512322399
58-59	15.49001519426959	30.803668330800953	18.14539468924101	35.56092178568844
60-61	12.115593342981185	28.168415340086835	19.039435600578873	40.676555716353114
62-63	15.460794067106809	29.444695667902685	17.787826716107443	37.306683548883065
64-65	15.72225789821188	27.93882220996174	17.911055235476606	38.427864656349776
66-67	12.553587902248431	37.805473653745274	13.522240109979561	36.11869833402673
68-69	14.065071528041525	41.26290850559745	11.532201182789866	33.13981878357116
70-71	14.194382652415316	41.97728464724287	11.376666124102508	32.451666576239305
72-73	16.693042982844844	36.53677461362465	12.841497933604028	33.92868446992648
74-75	14.939046447692087	34.42338301258863	16.998263637679063	33.639306902040225
76	12.274609805942886	39.33047582876675	13.710596278009874	34.68431808728049
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	0.0
17	0.0
18	1.0
19	5.0
20	7.0
21	6.0
22	8.0
23	13.5
24	19.5
25	22.0
26	39.0
27	64.0
28	79.5
29	87.0
30	109.0
31	155.5
32	218.0
33	256.0
34	322.5
35	470.0
36	634.0
37	717.0
38	833.0
39	1079.0
40	1351.5
41	1718.0
42	1942.0
43	2147.5
44	2503.0
45	2813.5
46	2974.0
47	3131.0
48	3360.0
49	3505.5
50	3579.0
51	3540.0
52	3452.5
53	3362.0
54	3320.0
55	3100.0
56	2826.0
57	2537.5
58	2303.0
59	2152.5
60	1871.0
61	1608.0
62	1476.0
63	1351.0
64	1099.5
65	840.0
66	631.0
67	555.0
68	491.5
69	386.0
70	310.0
71	276.0
72	253.5
73	196.5
74	134.5
75	107.0
76	109.5
77	94.0
78	67.5
79	59.0
80	56.5
81	50.0
82	35.0
83	24.0
84	24.5
85	23.0
86	19.0
87	17.0
88	10.5
89	7.5
90	10.0
91	11.0
92	13.0
93	9.0
94	4.5
95	6.0
96	8.0
97	6.0
98	6.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0018085471940390285
7	0.010851283164234171
8	0.0018085471940390285
9	0.014468377552312228
10-11	9.042735970195143E-4
12-13	0.0
14-15	9.042735970195143E-4
16-17	0.0
18-19	0.004521367985097571
20-21	0.0
22-23	0.004521367985097571
24-25	0.005425641582117086
26-27	0.0
28-29	0.005425641582117086
30-31	0.003617094388078057
32-33	0.0
34-35	0.005425641582117086
36-37	0.016276924746351257
38-39	0.010851283164234171
40-41	0.0
42-43	9.042735970195143E-4
44-45	0.0063299151791366
46-47	0.003617094388078057
48-49	0.0
50-51	0.004521367985097571
52-53	0.0126598303582732
54-55	0.005425641582117086
56-57	0.013564103955292714
58-59	0.016276924746351257
60-61	0.02351111352250737
62-63	0.014468377552312228
64-65	0.020798292731448828
66-67	0.018085471940390285
68-69	0.0
70-71	0.0
72-73	0.0063299151791366
74-75	0.009042735970195143
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	55293.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.94549038757167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.95119744446701	96.9182355813575
2	0.5779492955665935	1.1321505434684318
3	0.2492752552763262	0.7324616135858065
4	0.10340306885536496	0.4051145714647424
5	0.0590874679173514	0.28936755104624456
6	0.0295437339586757	0.17362053062774674
7	0.007385933489668925	0.0506393214330928
8	0.0036929667448344626	0.028936755104624456
9	0.0036929667448344626	0.032553849492702513
>10	0.01292538360692062	0.13744958674696614
>50	0.0018464833724172313	0.09947009567214656
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005425641582117086	0.0
2	0.0	0.0	0.0	0.005425641582117086	0.0
3	0.0	0.0	0.0	0.005425641582117086	0.0
4	0.0	0.0	0.0	0.005425641582117086	0.0
5	0.0	0.0	0.0	0.0126598303582732	0.0
6	0.0	0.0	0.0	0.014468377552312228	0.0
7	0.0	0.0	0.0	0.0253196607165464	0.0
8	0.0	0.0	0.0	0.0253196607165464	0.0
9	0.0	0.0	0.0	0.0253196607165464	0.0
10	0.0	0.0	0.0	0.0253196607165464	0.0
11	0.0	0.0	0.0	0.028936755104624456	0.0
12	0.0	0.0	0.0	0.028936755104624456	0.0
13	0.0	0.0	0.0	0.028936755104624456	0.0
14	0.0	0.0	0.0	0.028936755104624456	0.0
15	0.0	0.0	0.0	0.028936755104624456	0.0
16	0.0	0.0	0.0	0.028936755104624456	0.0
17	0.0	0.0	0.0	0.028936755104624456	0.0
18	0.0	0.0	0.0	0.028936755104624456	0.0
19	0.0	0.0	0.0	0.04521367985097571	0.0
20	0.0	0.0	0.0	0.04702222704501474	0.0
21	0.0	0.0	0.0	0.05244786862713183	0.0
22	0.0	0.0	0.0	0.07776752934367823	0.0
23	0.0	0.0	0.0	0.08500171811983434	0.0
24	0.0	0.0	0.0	0.10851283164234171	0.0
25	0.0	0.0	0.0	0.11212992603041977	0.0
26	0.0	0.0	0.0	0.11212992603041977	0.0
27	0.0	0.0	0.0	0.11755556761253685	0.0
28	0.0	0.0	0.0	0.12298120919465394	0.0
29	0.0	0.0	0.0	0.1392581339410052	0.0
30	0.0	0.0	0.0	0.14830086991120034	0.0
31	0.0	0.0	0.0	0.15372651149331742	0.0
32	0.0	0.0	0.0	0.16819488904562965	0.0
33	0.0	0.0	0.0	0.1772376250158248	0.0
34	0.0	0.0	0.0	0.1971316441502541	0.0
35	0.0	0.0	0.0	0.22425985206083954	0.0
36	0.0	0.0	0.0	0.27489917349393234	0.0
37	0.0	0.0	0.0	0.34181541967337636	0.0
38	0.0	0.0	0.0	0.4322427793753278	0.0
39	0.0	0.0	0.0	0.575118007704411	0.0
40	0.0	0.0	0.0	0.7523556327202359	0.0
41	0.0	0.0	0.0	0.889805219467202	0.0
42	0.0	0.0	0.0	1.0362975421843632	0.0
43	0.0	0.0	0.0	1.2370462807226954	0.0
44	0.0	0.0	0.0	1.3437505651709982	0.0
45	0.0	0.0	0.0	1.4902428878881595	0.0
46	0.0	0.0	0.0	1.6656719657099452	0.0
47	0.0	0.0	0.0	1.7579078726059356	0.0
48	0.0	0.0	0.0	1.8591865154721212	0.0
49	0.0	0.0	0.0	1.9604651583383068	0.0
50	0.0	0.0	0.0	2.0490839708462194	0.0
51	0.0	0.0	0.0	2.157596802488561	0.0
52	0.0	0.0	0.0	2.2715352757130196	0.0
53	0.0	0.0	0.0	2.352919899444776	0.0
54	0.0	0.0	0.0	2.5193062412963667	0.0
55	0.0	0.0	0.0	2.613350695386396	0.0
56	0.0	0.0	0.0	2.7128207910585425	0.0
57	0.0	0.0	0.0	2.8177165283128063	0.0
58	0.0	0.0	0.0	2.8882498688803286	0.0
59	0.0	0.0	0.0	2.9587832094478506	0.0
60	0.0	0.0	0.0	3.0220823612392165	0.0
61	0.0	0.0	0.0	3.116126815329246	0.0
62	0.0	0.0	0.0	3.1703832311504168	0.0
63	0.0	0.0	0.0	3.240916571717939	0.0
64	0.0	0.0	0.0	3.3295353842258515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCTC	15	0.0022136807	70.00001	38
GTTTAGA	35	3.6270649E-9	70.00001	36
GACGTTG	15	0.0022136807	70.00001	14
TTCCGAC	30	1.0063377E-7	70.00001	19
TCGATCA	15	0.0022136807	70.00001	39
AAAGTTA	15	0.0022136807	70.00001	45
GTGTCGC	15	0.0022136807	70.00001	36
GTGTAGC	30	1.0063377E-7	70.00001	36
GTATCAC	15	0.0022136807	70.00001	57
TAGTGTA	30	1.0063377E-7	70.00001	34
TAGCTAT	15	0.0022136807	70.00001	39
GCGTAGC	15	0.0022136807	70.00001	36
ATCTCTT	20	7.862112E-5	70.0	42
CGGCGGT	20	7.862112E-5	70.0	46
TGGCCGC	25	2.8053382E-6	70.0	49
TCGGCGG	20	7.862112E-5	70.0	45
GCTCTCG	20	7.862112E-5	70.0	41
CTAGTGT	20	7.862112E-5	70.0	33
TCTCGGC	20	7.862112E-5	70.0	43
TGTAGCT	25	2.8053382E-6	70.0	37
>>END_MODULE