SRR1779873_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779873_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55293
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	33502	60.589948094695536	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	4007	7.246848606514387	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	2012	3.638796954406525	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	932	1.6855659848443745	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	857	1.5499249452914472	TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT	576	1.0417231837664804	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT	506	0.9151248801837484	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	405	0.7324616135858065	No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	243	0.4394769681514839	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	242	0.43766842095744485	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAGCTCGTATGCCGT	208	0.37617781636011793	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATATCGTATGCCGT	202	0.3653265331958837	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	181	0.32734704212106414	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	144	0.2604307959416201	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCG	140	0.253196607165464	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	132	0.23872822961315174	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	128	0.23149404083699565	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT	121	0.21883421047872242	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGGATCTCGTATGCCGT	117	0.21160002170256634	No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT	116	0.2097914745085273	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTCTGCCGT	107	0.19351454976217605	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT	85	0.15372651149331742	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT	84	0.1519179642992784	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCG	78	0.14106668113504423	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCCCGTATCTCGTATGCCGT	74	0.1338324923588881	No Hit
CTTATACACCTCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	74	0.1338324923588881	No Hit
CTTATACACATCTCCGAGCCCACGAGAAGAGCACGTATCTCGTATGCCGT	73	0.13202394516484908	No Hit
TCTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	69	0.12478975638869295	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCC	69	0.12478975638869295	No Hit
TCCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	67	0.12117266200061491	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGC	67	0.12117266200061491	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGCATCTCGTATGCCGT	65	0.11755556761253685	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGC	59	0.10670428444830267	No Hit
CTTATACACATCTCCGAGCACACGAGACGAGCACGTATCTCGTATGCCGT	58	0.10489573725426365	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTACA	30	1.0014628E-7	70.04892	1
GAAAAAC	30	1.0014628E-7	70.04892	60
GCCGTCC	15	0.0022075465	70.04892	46
CGTCCTC	15	0.0022075465	70.04892	48
GCTTATA	15	0.0022075465	70.04892	1
TCTTTAT	90	0.0	70.04891	1
ACGTCTT	25	2.7936894E-6	70.048904	47
GACGTCT	25	2.7936894E-6	70.048904	46
TCTTAAT	25	2.7936894E-6	70.048904	1
AGCCCGT	15	0.002215498	69.98552	30
ACACGAG	15	0.002215498	69.98552	20
AACAAGC	15	0.002215498	69.98552	69
CACACGA	15	0.002215498	69.98552	19
AGAAGAG	15	0.002215498	69.98552	25
ACGCATC	15	0.002215498	69.98552	33
CACGCAT	15	0.002215498	69.98552	32
CATCTCG	15	0.002215498	69.98552	36
GGATCTC	20	7.870171E-5	69.98552	35
GAGCCCG	15	0.002215498	69.98552	29
GTCTGCC	30	1.0077747E-7	69.98552	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content