##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779873_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55293
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.60933572061563	34.0	34.0	34.0	34.0	34.0
2	33.698135387842946	34.0	34.0	34.0	34.0	34.0
3	33.80010127864286	34.0	34.0	34.0	34.0	34.0
4	33.57625739243665	34.0	34.0	34.0	34.0	34.0
5	33.64328215144774	34.0	34.0	34.0	34.0	34.0
6	37.39525798925723	38.0	38.0	38.0	37.0	38.0
7	37.40619969978117	38.0	38.0	38.0	37.0	38.0
8	37.31466912629085	38.0	38.0	38.0	37.0	38.0
9	37.25073698298157	38.0	38.0	38.0	37.0	38.0
10-11	37.15487493896153	38.0	38.0	38.0	36.5	38.0
12-13	37.31072649340784	38.0	38.0	38.0	36.5	38.0
14-15	37.34975494185521	38.0	38.0	38.0	37.0	38.0
16-17	37.048206825457115	38.0	38.0	38.0	35.5	38.0
18-19	36.830593384334364	38.0	38.0	38.0	34.0	38.0
20-21	36.9682057403288	38.0	37.5	38.0	35.0	38.0
22-23	37.1440236557973	38.0	38.0	38.0	36.0	38.0
24-25	37.050838261624435	38.0	38.0	38.0	35.5	38.0
26-27	36.8397446331362	38.0	38.0	38.0	34.0	38.0
28-29	36.17280668439043	38.0	37.0	38.0	33.0	38.0
30-31	35.76308031758089	38.0	36.5	38.0	31.0	38.0
32-33	35.941683395728205	38.0	37.0	38.0	31.0	38.0
34-35	36.3438409925307	38.0	37.0	38.0	34.0	38.0
36-37	35.802841227641835	38.0	36.5	38.0	32.5	38.0
38-39	32.69379487457725	36.5	30.5	37.0	18.0	38.0
40-41	35.424601667480516	37.5	35.5	38.0	30.5	38.0
42-43	36.723301322048	38.0	37.0	38.0	34.5	38.0
44-45	36.428562385835455	38.0	37.0	38.0	33.5	38.0
46-47	36.63532454379397	38.0	37.0	38.0	34.0	38.0
48-49	36.124247192230484	38.0	37.0	38.0	33.5	38.0
50-51	35.94656647315212	38.0	36.5	38.0	31.5	38.0
52-53	35.85935832745555	38.0	37.0	38.0	31.5	38.0
54-55	35.97128931329463	38.0	37.0	38.0	32.0	38.0
56-57	35.77939341327112	38.0	37.0	38.0	31.5	38.0
58-59	35.600482882100806	38.0	37.0	38.0	31.5	38.0
60-61	34.96599027001609	38.0	36.0	38.0	27.0	38.0
62-63	35.16543685457472	38.0	36.0	38.0	29.0	38.0
64-65	35.965031740003255	38.0	37.0	38.0	34.0	38.0
66-67	35.43040710397338	38.0	36.5	38.0	30.0	38.0
68-69	35.02768885754074	38.0	36.0	38.0	28.0	38.0
70-71	30.19752952453294	33.5	26.5	37.5	16.5	38.0
72-73	21.311033946430832	22.0	11.0	28.0	10.0	37.0
74-75	19.98517895574485	19.0	11.0	25.0	10.0	35.5
76	20.120666268786284	19.0	11.0	25.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	4.0
23	10.0
24	32.0
25	69.0
26	148.0
27	346.0
28	581.0
29	923.0
30	1346.0
31	1950.0
32	2984.0
33	4794.0
34	7915.0
35	17708.0
36	13290.0
37	3192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	3.9810357931309035	3.6969346024392893	12.979986247330896	79.34204335709892
2	1.7343967590834282	2.445155806340767	81.4461143363536	14.374333098222197
3	2.229938690250122	4.89573725426365	85.51534552294142	7.358978532544806
4	1.4323693776789106	71.63655435588592	21.619373157542547	5.311703108892626
5	1.5680104172318374	17.07449405892247	79.29394317544717	2.0635523483985314
6	2.931655001537265	79.79852784258405	12.088329444956866	5.181487710921816
7	2.7363319045810504	16.75799829996564	8.832944495686615	71.6727252997667
8	2.2245130486680047	79.82927314488272	4.018591865154721	13.927621941294557
9	2.228130143056083	13.748575769084695	2.3547284466388154	81.6685656412204
10-11	2.342068616280542	45.69656195178413	38.93802108766028	13.023348344275043
12-13	3.269853326822563	5.6752210948944715	46.65147487023674	44.40345070804623
14-15	4.041198705080209	3.33224820501691	10.784366918054726	81.84218617184816
16-17	42.878845423471326	38.07805689689472	2.431591702385474	16.611505977248477
18-19	45.69746622538115	11.924655923896333	2.2769609172951366	40.10091693342738
20-21	6.83359557267647	3.6550738791528765	2.2887164740563906	87.22261407411426
22-23	4.142477347946395	43.38976000578735	2.2290344166531026	50.23872822961315
24-25	42.99730526468088	42.69527788327637	2.2398856998173367	12.067531152225417
26-27	46.823286853670446	47.33781853037455	2.212757491906751	3.6261371240482525
28-29	42.19973595210967	12.197746550196227	2.4840395710126058	43.1184779266815
30-31	46.16045430705514	43.084115529994754	2.734523357387011	8.020906805563092
32-33	11.977103792523467	41.64541623713671	3.344003761778164	43.03347620856166
34-35	37.92523465899843	12.24838587162932	4.3396089920966485	45.486770477275606
36-37	10.982402835802	38.356573164776734	43.36534461866782	7.295679380753441
38-39	3.4932089052863837	11.899336263179789	44.946919139854955	39.660535691678874
40-41	3.068200314687212	3.5465610475105347	46.71206120123705	46.673177436565204
42-43	42.791517836958	3.155943391960935	42.809603472442014	11.242935298639056
44-45	6.657262221257663	41.70600256813701	46.95259797804424	4.684137232561084
46-47	42.752247119888594	5.774691190566618	12.346951693704447	39.12610999584034
48-49	41.042265747924695	2.5120720525202107	4.827012460890167	51.618649738664935
50-51	10.508845555515357	2.457400238775732	44.36344560616475	42.67030859954416
52-53	2.956899280900909	2.4847361042015286	82.39247433404188	12.165890280855683
54-55	2.818060920777658	2.6099857966110895	51.15300759021866	43.41894569239259
56-57	42.41461980368209	2.5331343013525127	13.317048898538925	41.735196996426474
58-59	6.3036497670842575	4.304644746958528	77.93496449730904	11.456740988648184
60-61	41.573277616476275	40.47069890284825	14.471910925388254	3.484112555287222
62-63	5.3611809613401595	88.10445574108581	2.634006910649999	3.900356386924037
64-65	1.842271863977571	92.52419281902867	2.3957673871755447	3.2377679298182147
66-67	1.8579155887620529	92.18571919604899	2.1980208766756517	3.758344338513306
68-69	1.9886954555731404	91.79832692742482	2.208455799231291	4.004521817770744
70-71	2.781093987410462	85.30135301353013	3.2966138484914262	8.620939150567976
72-73	5.834840810419681	67.97756874095514	6.362156295224312	19.825434153400867
74-75	7.513860123542765	60.41838128227622	8.319691776175961	23.748066818005046
76	7.346850187198177	61.67772974732768	7.478883683915425	23.49653638155872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	2.5
19	3.0
20	1.5
21	0.0
22	10.5
23	14.0
24	16.0
25	25.0
26	29.5
27	40.5
28	51.0
29	55.0
30	48.0
31	52.5
32	74.0
33	84.0
34	86.0
35	109.5
36	121.5
37	112.0
38	151.0
39	221.0
40	485.5
41	5670.5
42	10622.0
43	13107.0
44	13849.5
45	9595.5
46	7084.0
47	5201.5
48	2387.0
49	1120.0
50	785.0
51	605.0
52	338.5
53	236.0
54	220.0
55	200.0
56	173.5
57	174.0
58	181.0
59	169.5
60	174.5
61	142.0
62	93.0
63	102.5
64	98.5
65	74.5
66	62.0
67	60.0
68	57.5
69	49.0
70	31.0
71	19.0
72	28.0
73	31.5
74	23.0
75	20.0
76	14.0
77	11.0
78	10.5
79	7.0
80	6.5
81	9.5
82	9.5
83	6.0
84	4.5
85	3.5
86	2.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05606496301520988
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.042735970195143E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.019894019134429314
52-53	0.02803248150760494
54-55	0.0443094062539562
56-57	0.04611795344799523
58-59	0.02803248150760494
60-61	0.024415387119526885
62-63	0.028936755104624456
64-65	0.014468377552312228
66-67	0.028936755104624456
68-69	0.009947009567214657
70-71	0.016276924746351257
72-73	0.02351111352250737
74-75	0.015372651149331742
76	0.007234188776156114
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	55293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.330294974047348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08063578212831	7.098547736603186
2	9.052141887962783	1.6891830792324525
3	4.865283969761582	1.3618360371113885
4	2.8881566194999033	1.077894127647261
5	1.9965109517348323	0.9314018049300996
6	1.415002907540221	0.7921436709890946
7	0.7365768559798411	0.4810735536143816
8	0.3489048265167668	0.2604307959416201
9	0.21321961620469082	0.17904617220986382
>10	1.6669897266912193	2.9208037183730307
>50	0.3295212250436131	2.043658329264102
>100	0.27137042062415195	4.496048324381024
>500	0.07753440589261484	5.192338994086051
>1k	0.03876720294630742	10.885645560920912
>5k	0.0	0.0
>10k+	0.01938360147315371	60.589948094695536
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	33502	60.589948094695536	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	4007	7.246848606514387	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	2012	3.638796954406525	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	932	1.6855659848443745	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	857	1.5499249452914472	TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT	576	1.0417231837664804	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT	506	0.9151248801837484	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	405	0.7324616135858065	No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	243	0.4394769681514839	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	242	0.43766842095744485	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAGCTCGTATGCCGT	208	0.37617781636011793	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATATCGTATGCCGT	202	0.3653265331958837	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	181	0.32734704212106414	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	144	0.2604307959416201	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCG	140	0.253196607165464	No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	132	0.23872822961315174	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	128	0.23149404083699565	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT	121	0.21883421047872242	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGGATCTCGTATGCCGT	117	0.21160002170256634	No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT	116	0.2097914745085273	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTCTGCCGT	107	0.19351454976217605	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT	85	0.15372651149331742	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT	84	0.1519179642992784	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCG	78	0.14106668113504423	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCCCGTATCTCGTATGCCGT	74	0.1338324923588881	No Hit
CTTATACACCTCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	74	0.1338324923588881	No Hit
CTTATACACATCTCCGAGCCCACGAGAAGAGCACGTATCTCGTATGCCGT	73	0.13202394516484908	No Hit
TCTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	69	0.12478975638869295	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCC	69	0.12478975638869295	No Hit
TCCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	67	0.12117266200061491	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGC	67	0.12117266200061491	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGCATCTCGTATGCCGT	65	0.11755556761253685	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGC	59	0.10670428444830267	No Hit
CTTATACACATCTCCGAGCACACGAGACGAGCACGTATCTCGTATGCCGT	58	0.10489573725426365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005425641582117086	0.0
2	0.0	0.0	0.0	0.005425641582117086	0.0
3	0.0	0.0	0.0	0.005425641582117086	0.0
4	0.0	0.0	0.0	0.005425641582117086	0.0
5	0.0	0.0	0.0	0.014468377552312228	0.0
6	0.0	0.0	0.0	0.016276924746351257	0.0
7	0.0	0.0	0.0	0.027128207910585428	0.0
8	0.0	0.0	0.0	0.027128207910585428	0.0
9	0.0	0.0	0.0	0.028936755104624456	0.0
10	0.0	0.0	0.0	0.028936755104624456	0.0
11	0.0	0.0	0.0	0.032553849492702513	0.0
12	0.0	0.0	0.0	0.032553849492702513	0.0
13	0.0	0.0	0.0	0.032553849492702513	0.0
14	0.0	0.0	0.0	0.032553849492702513	0.0
15	0.0	0.0	0.0	0.032553849492702513	0.0
16	0.0	0.0	0.0	0.032553849492702513	0.0
17	0.0	0.0	0.0	0.032553849492702513	0.0
18	0.0	0.0	0.0	0.032553849492702513	0.0
19	0.0	0.0	0.0	0.03978803826885863	0.0
20	0.0	0.0	0.0	0.03978803826885863	0.0
21	0.0	0.0	0.0	0.04521367985097571	0.0
22	0.0	0.0	0.0	0.04521367985097571	0.0
23	0.0	0.0	0.0	0.054256415821170856	0.0
24	0.0	0.0	0.0	0.054256415821170856	0.0
25	0.0	0.0	0.0	0.05787351020924891	0.0
26	0.0	0.0	0.0	0.05787351020924891	0.0
27	0.0	0.0	0.0	0.06149060459732697	0.0
28	0.0	0.0	0.0	0.06510769898540503	0.0
29	0.0	0.0	0.0	0.08138462373175628	0.0
30	0.0	0.0	0.0	0.0886188125079124	0.0
31	0.0	0.0	0.0	0.09585300128406851	0.0
32	0.0	0.0	0.0	0.11212992603041977	0.0
33	0.0	0.0	0.0	0.126598303582732	0.0
34	0.0	0.0	0.0	0.14830086991120034	0.0
35	0.0	0.0	0.0	0.17000343623966868	0.0
36	0.0	0.0	0.0	0.23330258803103468	0.0
37	0.0	0.0	0.0	0.3454325140614544	0.0
38	0.0	0.0	0.0	0.4322427793753278	0.0
39	0.0	0.0	0.0	0.573309460510372	0.0
40	0.0	0.0	0.0	0.7342701607798455	0.0
41	0.0	0.0	0.0	0.8536342755864215	0.0
42	0.0	0.0	0.0	0.9947009567214656	0.0
43	0.0	0.0	0.0	1.200875336841915	0.0
44	0.0	0.0	0.0	1.2967283381259833	0.0
45	0.0	0.0	0.0	1.4215180945146764	0.0
46	0.0	0.0	0.0	1.596947172336462	0.0
47	0.0	0.0	0.0	1.6819488904562965	0.0
48	0.0	0.0	0.0	1.7741847973522868	0.0
49	0.0	0.0	0.0	1.8754634402184724	0.0
50	0.0	0.0	0.0	1.9622737055323458	0.0
51	0.0	0.0	0.0	2.0490839708462194	0.0
52	0.0	0.0	0.0	2.153979708100483	0.0
53	0.0	0.0	0.0	2.220895954279927	0.0
54	0.0	0.0	0.0	2.3800481073553614	0.0
55	0.0	0.0	0.0	2.454198542310962	0.0
56	0.0	0.0	0.0	2.544625902012913	0.0
57	0.0	0.0	0.0	2.6368618089089035	0.0
58	0.0	0.0	0.0	2.7019695078943085	0.0
59	0.0	0.0	0.0	2.776119942849909	0.0
60	0.0	0.0	0.0	2.8358020002531967	0.0
61	0.0	0.0	0.0	2.931655001537265	0.0
62	0.0	0.0	0.0	2.97867722858228	0.0
63	0.0	0.0	0.0	3.041976380373646	0.0
64	0.0	0.0	0.0	3.1233610041054023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTACA	30	1.0014628E-7	70.04892	1
GAAAAAC	30	1.0014628E-7	70.04892	60
GCCGTCC	15	0.0022075465	70.04892	46
CGTCCTC	15	0.0022075465	70.04892	48
GCTTATA	15	0.0022075465	70.04892	1
TCTTTAT	90	0.0	70.04891	1
ACGTCTT	25	2.7936894E-6	70.048904	47
GACGTCT	25	2.7936894E-6	70.048904	46
TCTTAAT	25	2.7936894E-6	70.048904	1
AGCCCGT	15	0.002215498	69.98552	30
ACACGAG	15	0.002215498	69.98552	20
AACAAGC	15	0.002215498	69.98552	69
CACACGA	15	0.002215498	69.98552	19
AGAAGAG	15	0.002215498	69.98552	25
ACGCATC	15	0.002215498	69.98552	33
CACGCAT	15	0.002215498	69.98552	32
CATCTCG	15	0.002215498	69.98552	36
GGATCTC	20	7.870171E-5	69.98552	35
GAGCCCG	15	0.002215498	69.98552	29
GTCTGCC	30	1.0077747E-7	69.98552	42
>>END_MODULE