##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779872_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174917
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.886717700395046	34.0	23.0	34.0	12.0	34.0
2	29.73570893623833	34.0	31.0	34.0	12.0	34.0
3	29.30251490707021	34.0	27.0	34.0	11.0	34.0
4	29.53974742306351	34.0	27.0	34.0	12.0	34.0
5	29.83608797315298	34.0	31.0	34.0	11.0	34.0
6	32.517736983826616	37.0	31.0	38.0	12.0	38.0
7	32.62738899020678	37.0	32.0	38.0	12.0	38.0
8	32.03520526878462	37.0	31.0	38.0	11.0	38.0
9	33.02624101716814	37.0	34.0	38.0	12.0	38.0
10-11	31.589376675794803	37.0	28.5	38.0	11.5	38.0
12-13	32.4514026652641	37.0	31.0	38.0	11.0	38.0
14-15	31.227742300634013	37.0	27.0	38.0	11.0	38.0
16-17	31.84448624204623	37.0	28.5	38.0	11.0	38.0
18-19	32.700529393941125	37.5	32.0	38.0	11.0	38.0
20-21	31.44885574300954	37.0	27.5	38.0	11.0	38.0
22-23	32.31960587021273	37.0	31.0	38.0	11.0	38.0
24-25	32.62204645631928	37.5	32.0	38.0	11.0	38.0
26-27	31.54401801997519	37.0	26.5	38.0	11.0	38.0
28-29	32.74082279023766	37.5	32.5	38.0	11.0	38.0
30-31	33.11653527101426	38.0	34.0	38.0	11.0	38.0
32-33	33.25571270945649	38.0	34.0	38.0	11.0	38.0
34-35	33.19651606190365	38.0	34.0	38.0	11.0	38.0
36-37	32.68265520218161	38.0	32.5	38.0	11.0	38.0
38-39	32.6042265760332	37.5	32.5	38.0	11.0	38.0
40-41	32.60796835070347	37.5	32.5	38.0	11.0	38.0
42-43	33.01038206692317	38.0	33.5	38.0	11.0	38.0
44-45	33.05104135104078	38.0	34.0	38.0	11.0	38.0
46-47	32.94037457765683	38.0	34.0	38.0	11.0	38.0
48-49	32.65798350074607	37.5	32.5	38.0	11.0	38.0
50-51	32.04504136247478	37.5	29.5	38.0	11.0	38.0
52-53	32.603288988491684	37.0	32.0	38.0	11.0	38.0
54-55	32.75358026949925	37.5	33.0	38.0	11.0	38.0
56-57	32.808431999176754	38.0	33.5	38.0	11.0	38.0
58-59	33.00230966687057	38.0	34.0	38.0	11.0	38.0
60-61	32.90861951668506	38.0	34.0	38.0	11.0	38.0
62-63	32.882418518497346	38.0	34.0	38.0	11.0	38.0
64-65	32.89587347141787	38.0	33.5	38.0	11.0	38.0
66-67	32.745459274970415	38.0	33.0	38.0	11.0	38.0
68-69	32.10118513352047	37.0	31.0	38.0	11.0	38.0
70-71	32.14729271597386	37.0	31.0	38.0	11.0	38.0
72-73	32.345218017688396	37.0	31.0	38.0	11.0	38.0
74-75	32.41173528016145	37.0	31.5	38.0	11.0	38.0
76	31.50577130867783	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	45.0
13	334.0
14	1466.0
15	2720.0
16	3654.0
17	4237.0
18	4353.0
19	3942.0
20	3172.0
21	2490.0
22	2001.0
23	1763.0
24	1747.0
25	1781.0
26	1905.0
27	2210.0
28	2612.0
29	3154.0
30	3751.0
31	4595.0
32	6016.0
33	7603.0
34	10513.0
35	15988.0
36	28037.0
37	54826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.222128209379306	18.796343408588072	9.756055729288748	33.22547265274387
2	20.08552627817765	21.301531583551057	32.32447389333227	26.28846824493903
3	18.11544904154542	23.320203296420587	31.00270413967768	27.561643522356317
4	13.596162751476415	21.556509658866776	29.695798578754495	35.151529010902316
5	13.503547396765322	31.741340178484652	32.957345483858056	21.797766940891965
6	26.775484534903665	39.123549253902006	16.53536104282202	17.565605168372304
7	26.01715265866209	29.474556889651225	20.435105774728417	24.073184676958263
8	22.865783937065494	36.24908525429931	19.404043175997074	21.481087632638125
9	22.97757016826662	18.42871109942196	19.54362753786428	39.05009119444715
10-11	23.02263157443378	26.250568147709828	24.532546645284302	26.194253632572085
12-13	23.32363349474322	22.759365870670088	26.048640212214934	27.86836042237175
14-15	24.412003475954172	24.99628393057239	22.773788561366597	27.817924032106838
16-17	25.230754278494473	26.276687886436594	22.83931384011137	25.653243994957563
18-19	22.167210274910452	25.831063137643895	25.490891107788794	26.51083547965685
20-21	24.035685496549792	25.199094427642827	24.954406947295002	25.81081312851238
22-23	25.285503157329035	25.213466315629994	23.75700715508789	25.744023371953084
24-25	22.367789148705047	27.999256760619744	23.351152021039393	26.281802069635813
26-27	23.59974844924678	27.82265671897779	24.393848440671185	24.18374639110425
28-29	22.25049169830307	27.206353199469426	25.538581164524537	25.00457393770297
30-31	22.866469809708114	26.524235988234317	25.504314940642768	25.104979261414794
32-33	21.302960832852154	27.36497881852536	26.029202421720015	25.302857926902472
34-35	21.68723950442235	28.261304579004843	25.21711538131693	24.834340535255876
36-37	23.69329212329844	26.124722304025983	25.243243088694022	24.938742483981553
38-39	23.785074114838693	25.16016523964036	26.39717548850041	24.657585157020538
40-41	21.76603760640761	26.924484183927234	25.95316635890165	25.356311850763507
42-43	20.38692858286263	26.227160465594913	26.6393583205653	26.746552630977156
44-45	20.681848067830334	24.557050318169086	28.11322588575643	26.647875728244152
46-47	20.881310388199644	25.631467611914697	27.262878051569377	26.224343948316275
48-49	20.82702081558682	25.7905177884368	27.487036708839053	25.895424687137332
50-51	22.85628528549989	25.92124633745444	25.87922532694919	25.343243050096476
52-53	18.750732618012254	27.060121850457296	26.896301315424576	27.292844216105877
54-55	19.71675162021618	25.95461965300072	26.807680982507097	27.520947744275997
56-57	20.13848982054075	26.525775288544285	26.070908846594794	27.264826044320174
58-59	19.524788367578573	26.292165669375983	26.756742075755973	27.426303887289478
60-61	19.283226680848877	25.831632040350666	26.707440511931924	28.177700766868536
62-63	20.20595037636545	26.18005506113616	25.84614329046036	27.767851272038037
64-65	20.56939766339374	25.64319576368596	25.240322755891555	28.547083817028746
66-67	19.792315917681165	28.03106112156266	23.827345452050253	28.34927750870592
68-69	20.52808054128684	28.353814534363543	23.338278550051026	27.779826374298594
70-71	21.076688238926113	28.515973381508843	23.08593839328592	27.321399986279125
72-73	21.89115171751361	27.330993001875314	23.13440515940173	27.64345012120935
74-75	21.6702210990343	27.310619271693948	24.06074362918027	26.958416000091486
76	21.26113455227379	27.971024435982756	23.2667833007444	27.50105771099905
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.5
11	1.5
12	1.0
13	2.5
14	7.0
15	15.0
16	21.5
17	23.0
18	32.5
19	72.0
20	115.0
21	128.0
22	215.0
23	342.0
24	447.0
25	512.0
26	625.0
27	879.5
28	1173.5
29	1326.0
30	1500.0
31	1894.5
32	2411.5
33	2708.0
34	3058.0
35	3796.5
36	4667.5
37	5150.0
38	5567.5
39	6428.0
40	7405.0
41	8137.5
42	8336.0
43	8563.0
44	9079.0
45	9461.5
46	9555.0
47	9547.5
48	9500.0
49	9310.0
50	9160.0
51	8693.0
52	7903.5
53	7228.5
54	6876.0
55	6479.5
56	5665.5
57	4920.0
58	4592.0
59	4322.0
60	3820.0
61	3250.5
62	2913.0
63	2617.5
64	2190.0
65	1945.0
66	1706.0
67	1580.0
68	1510.5
69	1296.0
70	1124.5
71	1098.0
72	974.5
73	809.5
74	746.5
75	725.0
76	666.0
77	568.5
78	464.0
79	398.0
80	376.5
81	337.0
82	271.5
83	224.0
84	200.0
85	154.0
86	117.5
87	103.0
88	85.5
89	57.5
90	39.0
91	30.5
92	30.0
93	23.0
94	14.5
95	9.0
96	5.0
97	5.5
98	7.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.004001898043071857
7	0.009718895247460224
8	0.0028584986021941835
9	0.009147195527021387
10-11	0.003716048182852439
12-13	0.0
14-15	5.716997204388367E-4
16-17	8.575495806582551E-4
18-19	0.0031443484624136017
20-21	0.0
22-23	0.003716048182852439
24-25	0.004001898043071857
26-27	0.0011433994408776734
28-29	0.007432096365704878
30-31	8.575495806582551E-4
32-33	0.0
34-35	0.005716997204388367
36-37	0.023153838677772886
38-39	0.011148144548557315
40-41	0.0
42-43	5.716997204388367E-4
44-45	0.005716997204388367
46-47	0.004001898043071857
48-49	0.0
50-51	0.002572648741974765
52-53	0.01743684147338452
54-55	0.010004745107679642
56-57	0.01743684147338452
58-59	0.015150042591629173
60-61	0.028584986021941833
62-63	0.011719844268996152
64-65	0.027441586581064163
66-67	0.02058118993579812
68-69	2.8584986021941834E-4
70-71	5.716997204388367E-4
72-73	0.007432096365704878
74-75	0.01029059496789906
76	0.006288696924827203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	174917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.66247232158376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9354166736809	49.95380139915599
2	12.497983259865073	16.662929262727456
3	6.538476717477285	13.076130696124483
4	3.1999589034031413	8.532686873132699
5	1.4617347295733976	4.872142547584218
6	0.608907416041847	2.435476426097803
7	0.3044053821484386	1.4204690753407994
8	0.1695482291052529	0.9042003303921973
9	0.09220957072169814	0.5532226160418291
>10	0.19135911798297403	1.5889407734024985
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0028584986021941835	0.0
2	0.0	0.0	0.0	0.0034301983226330203	0.0
3	0.0	0.0	0.0	0.0034301983226330203	0.0
4	0.0	0.0	0.0	0.01600759217228743	0.0
5	0.0	0.0	0.0	0.018866090774481613	0.0
6	0.0	0.0	0.0	0.020581189935798123	0.0
7	0.0	0.0	0.0	0.022867988817553468	0.0
8	0.0	0.0	0.0	0.024011388258431143	0.0
9	0.0	0.0	0.0	0.024583087978869978	0.0
10	0.0	0.0	0.0	0.027441586581064163	0.0
11	0.0	0.0	0.0	0.028584986021941837	0.0
12	0.0	0.0	0.0	0.028584986021941837	0.0
13	0.0	0.0	0.0	0.029156685742380672	0.0
14	0.0	0.0	0.0	0.029156685742380672	0.0
15	0.0	0.0	0.0	0.029728385462819508	0.0
16	0.0	0.0	0.0	0.029728385462819508	0.0
17	0.0	0.0	0.0	0.03373028350589136	0.0
18	0.0	0.0	0.0	0.034301983226330206	0.0
19	0.0	0.0	0.0	0.03658878210808555	0.0
20	0.0	0.0	0.0	0.03716048182852438	0.0
21	0.0	0.0	0.0	0.0388755809898409	0.0
22	0.0	0.0	0.0	0.058313371484761345	0.0
23	0.0	0.0	0.0	0.058313371484761345	0.0
24	0.0	0.0	0.0	0.06117187008695553	0.0
25	5.716997204388367E-4	0.0	0.0	0.0623152695278332	0.0
26	5.716997204388367E-4	0.0	0.0	0.06803226673222157	0.0
27	5.716997204388367E-4	0.0	0.0	0.10633614800162362	0.0
28	5.716997204388367E-4	0.0	0.0	0.1389230320666373	0.0
29	5.716997204388367E-4	0.0	0.0	0.16121932116375196	0.0
30	5.716997204388367E-4	0.0	0.0	0.19266280578788797	0.0
31	5.716997204388367E-4	0.0	0.0	0.23554028482080072	0.0
32	5.716997204388367E-4	0.0	0.0	0.27270076664932513	0.0
33	5.716997204388367E-4	0.0	0.0	0.32358204176838157	0.0
34	5.716997204388367E-4	0.0	0.0	0.3881841101779701	0.0
35	5.716997204388367E-4	0.0	0.0	0.4785126660073063	0.0
36	5.716997204388367E-4	0.0	0.0	0.6814660667630934	0.0
37	5.716997204388367E-4	0.0	0.0	0.9890405163591875	0.0
38	5.716997204388367E-4	0.0	0.0	1.400092615354711	0.0
39	5.716997204388367E-4	0.0	0.0	1.824293807920328	0.0
40	5.716997204388367E-4	0.0	0.0	2.3937067294774095	0.0
41	5.716997204388367E-4	0.0	0.0	3.133486167725264	0.0
42	5.716997204388367E-4	0.0	0.0	4.072217108685834	0.0
43	5.716997204388367E-4	0.0	0.0	5.016665046850792	0.0
44	5.716997204388367E-4	0.0	0.0	5.783886071679711	0.0
45	5.716997204388367E-4	0.0	0.0	6.511088116077911	0.0
46	5.716997204388367E-4	0.0	0.0	7.282882738670341	0.0
47	5.716997204388367E-4	0.0	0.0	8.028950873843023	0.0
48	5.716997204388367E-4	0.0	0.0	8.655533767443988	0.0
49	5.716997204388367E-4	0.0	0.0	9.22952028676458	0.0
50	5.716997204388367E-4	0.0	0.0	9.790929412235517	0.0
51	5.716997204388367E-4	0.0	0.0	10.554720238741803	0.0
52	5.716997204388367E-4	0.0	0.0	11.44142650514244	0.0
53	5.716997204388367E-4	0.0	0.0	12.426465123458554	0.0
54	5.716997204388367E-4	0.0	0.0	13.231418329836437	0.0
55	5.716997204388367E-4	0.0	0.0	13.998639354665356	0.0
56	5.716997204388367E-4	0.0	0.0	14.682392220310204	0.0
57	5.716997204388367E-4	0.0	0.0	15.250661742426407	0.0
58	5.716997204388367E-4	0.0	0.0	15.752614096971707	0.0
59	5.716997204388367E-4	0.0	0.0	16.24656265543086	0.0
60	5.716997204388367E-4	0.0	0.0	16.717643225072464	0.0
61	5.716997204388367E-4	0.0	0.0	17.204159687165912	0.0
62	5.716997204388367E-4	0.0	0.0	17.729551730249202	0.0
63	5.716997204388367E-4	0.0	0.0	18.289817456279263	0.0
64	5.716997204388367E-4	0.0	0.0	18.878668168331266	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATTCG	15	0.0022185019	70.02575	31
AAGTACG	15	0.0022235431	69.9857	24
TACGCTT	15	0.0022260668	69.96569	14
TGCGATG	15	0.0022260668	69.96569	2
ACGTTGC	25	2.3912007E-4	55.972557	15
CAACCGT	20	0.0069478415	52.48928	6
GACGGGT	20	0.0069478415	52.48928	23
TTCAACG	20	0.0069557047	52.474274	3
GCCGACT	40	7.472827E-7	52.474274	20
ACGTGTA	35	2.075627E-5	50.018394	34
CGATTTC	30	5.8550294E-4	46.683834	42
CGTGTAG	40	4.5688204E-5	43.766098	35
TCTTACG	40	4.5842848E-5	43.741066	11
TGGTCGC	65	9.909854E-9	43.09277	49
CCGTACC	35	0.0012505858	40.014717	55
TTGTGCA	35	0.0012558758	39.980396	2
GATACAC	45	9.196453E-5	38.86983	3
CGACGAG	45	9.196453E-5	38.86983	22
CGACGAT	65	4.857302E-7	37.673836	22
GTGTAGA	180	0.0	36.958035	36
>>END_MODULE