FastQCFastQC Report
Wed 25 May 2016
SRR1779872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174917
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1828310.45238598878325No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG22721.298901764837037No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC10790.6168639983535048No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT6390.3653161213604167RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC5860.3350160361771583No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT3510.20066660187403168No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT3430.196093004110521No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2340.1337777345826878No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2140.12234374017391104No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT1820.10404934911986828No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAG150.002216956670.03805564
CGTCAGT150.002216956670.03805565
ATCGTAT501.4551915E-1162.9801939
AACTCGT653.6379788E-1253.82922437
TCTCGTA25500.053.1009438
ATCTCGT25650.052.7904137
GATCTCG25550.052.586236
TCGTATG27700.052.54664640
CTCGTAT27050.052.51582739
GCCGTCT27550.052.4967646
ATATCGT407.463768E-752.4834937
CGAGCGA200.006950875352.4834910
GATTACC200.006950875352.4834918
TAGCTCG200.006950875352.4834936
TGGTCGA200.006950875352.4834931
CGTCTTC27450.052.4328648
CGTATGC27700.052.42033441
TATGCCG27500.052.41987243
CCGTCTT27550.052.3696547
ATGCCGT27600.052.22994644