##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779871_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1922526
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.806939932151764	34.0	32.0	34.0	21.0	34.0
2	31.855540575264	34.0	34.0	34.0	24.0	34.0
3	31.554184442759162	34.0	34.0	34.0	23.0	34.0
4	31.65810761466945	34.0	34.0	34.0	24.0	34.0
5	32.43658031152765	34.0	34.0	34.0	31.0	34.0
6	35.21350400462725	38.0	36.0	38.0	27.0	38.0
7	35.45466225164185	38.0	37.0	38.0	31.0	38.0
8	34.4777194170586	38.0	35.0	38.0	24.0	38.0
9	35.42994321013084	38.0	37.0	38.0	31.0	38.0
10-11	33.9928003574464	37.5	34.5	38.0	19.5	38.0
12-13	35.05478391449583	38.0	36.0	38.0	27.0	38.0
14-15	33.73472478395611	37.5	34.0	38.0	18.0	38.0
16-17	34.5079616608566	38.0	35.0	38.0	26.0	38.0
18-19	35.580551576415616	38.0	36.5	38.0	31.0	38.0
20-21	34.02135315725249	38.0	35.0	38.0	19.0	38.0
22-23	35.060184881764926	38.0	36.5	38.0	27.5	38.0
24-25	35.38759215740125	38.0	36.5	38.0	29.0	38.0
26-27	34.23887479285066	38.0	34.5	38.0	23.5	38.0
28-29	35.68061342213318	38.0	36.5	38.0	31.5	38.0
30-31	36.10608101008777	38.0	37.0	38.0	34.0	38.0
32-33	36.19993305682212	38.0	37.0	38.0	34.0	38.0
34-35	36.09388169522805	38.0	37.0	38.0	33.0	38.0
36-37	35.554935537932906	38.0	37.0	38.0	29.5	38.0
38-39	35.51460110292396	38.0	37.0	38.0	29.5	38.0
40-41	35.52300619081355	38.0	37.0	38.0	29.0	38.0
42-43	35.92492273186423	38.0	37.0	38.0	32.5	38.0
44-45	35.96051496832813	38.0	37.0	38.0	33.0	38.0
46-47	35.87324826816386	38.0	37.0	38.0	32.0	38.0
48-49	35.62106728335534	38.0	37.0	38.0	29.5	38.0
50-51	34.94009027706257	38.0	36.0	38.0	27.5	38.0
52-53	35.52495336864105	38.0	36.5	38.0	31.0	38.0
54-55	35.6951833161164	38.0	37.0	38.0	32.5	38.0
56-57	35.75009154622616	38.0	37.0	38.0	31.5	38.0
58-59	36.05109553784968	38.0	37.0	38.0	34.0	38.0
60-61	35.869897468226696	38.0	37.0	38.0	32.0	38.0
62-63	35.92551388121669	38.0	37.0	38.0	32.5	38.0
64-65	35.933012609452355	38.0	37.0	38.0	32.0	38.0
66-67	35.654030686711124	38.0	37.0	38.0	31.0	38.0
68-69	34.97772825959181	38.0	36.0	38.0	27.0	38.0
70-71	35.04189176115173	38.0	36.0	38.0	27.5	38.0
72-73	35.381842690293915	38.0	36.5	38.0	31.0	38.0
74-75	35.49494389152604	38.0	36.5	38.0	30.0	38.0
76	34.49472828976045	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	52.0
13	559.0
14	1599.0
15	2571.0
16	3601.0
17	4431.0
18	5086.0
19	5543.0
20	6031.0
21	6608.0
22	7688.0
23	9113.0
24	10936.0
25	14080.0
26	17106.0
27	21468.0
28	26406.0
29	32864.0
30	41097.0
31	52583.0
32	67973.0
33	91520.0
34	128647.0
35	197949.0
36	365617.0
37	801395.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.42692693603867	18.38357032250883	10.595566966498675	26.593935774953824
2	22.587991007663877	22.43121809535996	31.130346221585558	23.850444675390605
3	20.63800437549349	22.091196686026613	30.68119754947397	26.589601389005924
4	14.694833775980143	18.632101724502036	34.799893473482285	31.87317102603554
5	14.456865602857908	32.812039993217255	35.33179785344905	17.39929655047578
6	28.467224285550806	37.044723947442336	19.526127981856582	14.961923785150278
7	27.46276001406578	30.72483504959436	21.908441340909988	19.903963595429868
8	24.732987147283733	34.5672474984044	20.73782050515144	19.961944849160428
9	24.51941033934931	18.786095594607783	21.780540865597196	34.91395320044571
10-11	24.229318473287368	25.151647320466036	26.209192806859715	24.409841399386885
12-13	24.860768751030548	23.69089856495716	26.78691156039388	24.661421123618414
14-15	23.340320726550186	24.850611960405928	24.788037513458967	27.021029799584916
16-17	23.50340869399673	27.491787431069948	25.155805390919845	23.84899848401348
18-19	22.058468210189687	27.246889212721076	27.244574474365834	23.450068102723407
20-21	22.88764885364359	26.81578818699981	27.437834390796272	22.85872856856032
22-23	23.297053748887503	27.084329227345762	26.45628530276305	23.162331721003685
24-25	22.58670428525208	27.67941391575559	26.445288157740055	23.288593641252277
26-27	22.716801100227773	27.316566719254144	26.79118611024852	23.175446070269558
28-29	22.31877747884402	27.375977170061404	27.289080040033543	23.016165311061034
30-31	22.439763892977854	27.460185028176586	27.060942992471333	23.039108086374224
32-33	21.95616283593859	27.706312118860417	27.30647315716215	23.03105188803885
34-35	22.256345275361593	27.285308257050623	27.576550596269144	22.881795871318637
36-37	22.925906011846653	27.30671153317398	26.881587849833505	22.88579460514586
38-39	22.653900555003855	27.268953441372418	27.311401950446566	22.765744053177162
40-41	22.22432595789038	27.092139114260245	27.454582716516878	23.2289522113325
42-43	21.736988761052604	26.577065098717252	27.83544541487669	23.850500725353456
44-45	21.793787426707116	25.953561753286476	28.616501874716505	23.636148945289907
46-47	21.398323709374296	25.986509985388505	28.80121240557016	23.81395389966704
48-49	21.02210146633103	26.168577223794088	29.179131863161857	23.630189446713025
50-51	21.447780238140197	26.296540772054446	28.90150799929882	23.354170990506535
52-53	20.128238542103976	26.661220001071644	28.96577860526865	24.24476285155573
54-55	20.332770219744347	26.742589464183762	28.63363673841488	24.291003577657005
56-57	19.987067292735937	26.668844660810358	28.709387605617977	24.634700440835726
58-59	19.668162988634784	26.264742343553372	29.32378350792735	24.74331115988449
60-61	20.165001726030226	26.20217940256722	29.179588047182147	24.453230824220405
62-63	20.252679580256316	26.87586224117159	28.32273674047347	24.548721438098628
64-65	20.64687902397149	26.2912997671787	27.51125736508721	25.550563843762603
66-67	20.658404206753122	26.388479552167567	27.172004229644187	25.781112011435127
68-69	20.85988271631267	26.311717237497906	27.030202057926118	25.79819798826331
70-71	21.597861971715236	26.233108749510407	26.797212407263594	25.37181687151076
72-73	21.973678495417627	26.60526143993991	26.047969213811445	25.37309085083102
74-75	22.28005661829004	26.60874961700463	25.867910424080563	25.243283340624767
76	22.394818740711806	26.428177960922085	25.871653453609344	25.305349844756762
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.0
4	2.0
5	1.0
6	1.5
7	4.5
8	6.0
9	6.5
10	15.5
11	32.0
12	40.0
13	73.5
14	173.0
15	343.0
16	647.0
17	847.0
18	1169.0
19	1942.0
20	3016.5
21	3640.0
22	4573.5
23	6827.0
24	9752.0
25	11357.0
26	13149.0
27	17010.0
28	21816.0
29	24553.0
30	27193.0
31	32518.0
32	38951.5
33	42700.0
34	46755.0
35	54581.5
36	62253.5
37	66154.0
38	70872.5
39	79817.5
40	87288.5
41	91862.0
42	93191.0
43	95570.5
44	99628.5
45	101219.5
46	101132.0
47	100373.0
48	97252.0
49	92261.0
50	89632.0
51	86497.5
52	79821.5
53	73612.5
54	70945.0
55	68036.0
56	61038.5
57	52875.5
58	48801.0
59	44596.0
60	36647.5
61	29667.0
62	26430.0
63	23874.5
64	19101.0
65	15395.5
66	12710.0
67	11512.0
68	10678.5
69	8868.0
70	7205.0
71	6519.0
72	5983.0
73	5040.5
74	4287.5
75	3941.0
76	3566.5
77	2961.0
78	2492.0
79	2254.0
80	1994.5
81	1627.0
82	1378.5
83	1238.0
84	1079.5
85	791.5
86	557.0
87	452.0
88	377.5
89	255.0
90	172.5
91	120.5
92	103.0
93	82.5
94	51.5
95	29.5
96	18.0
97	19.5
98	17.5
99	36.0
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.600745061445203E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0034329834811076673
7	0.0067619371597575275
8	0.0027567897651319152
9	0.010402980245780812
10-11	0.002886827018204175
12-13	2.600745061445203E-4
14-15	8.322384196624649E-4
16-17	8.062309690480129E-4
18-19	0.0032509313268065036
20-21	0.0
22-23	0.002548730160216299
24-25	0.003745072888481092
26-27	7.802235184335609E-4
28-29	0.007178056369588759
30-31	4.1611920983123247E-4
32-33	1.0402980245780812E-4
34-35	0.003745072888481092
36-37	0.01973965501636909
38-39	0.0102469355420941
40-41	0.0010402980245780812
42-43	7.802235184335609E-4
44-45	0.005305519925348214
46-47	0.0034849983823365717
48-49	3.3809685798787635E-4
50-51	0.0023406705553006826
52-53	0.01295171040599711
54-55	0.009284659869359373
56-57	0.013939993529346287
58-59	0.013367829615828343
60-61	0.02525323454663292
62-63	0.010923129258069852
64-65	0.02345872045423573
66-67	0.019687640115140185
68-69	4.1611920983123247E-4
70-71	3.641043086023284E-4
72-73	0.005487572079649378
74-75	0.009310667319973827
76	0.003068879172505339
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	1922526.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.57858476126322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.7129694691748	42.12978645239849
2	15.312642805764673	18.24611174644396
3	7.246795951321608	12.952615405001808
4	3.3383820815344434	7.955843192407286
5	1.4739187006951673	4.390699512028898
6	0.6288434541333908	2.247936182019106
7	0.2935003891541732	1.2240436468879954
8	0.1670405916572845	0.7961633638900065
9	0.1092802162019401	0.5859684561344813
>10	0.6748466332467081	7.719560155783539
>50	0.038694035634174465	1.5177309939637182
>100	0.0030856714816892795	0.23354089304071876
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.842533208913689E-4	0.0
2	0.0	0.0	0.0	0.0013003725307226014	0.0
3	0.0	0.0	0.0	0.001820521543011642	0.0
4	0.0	0.0	0.0	0.0023406705553006826	0.0
5	0.0	0.0	0.0	0.002548730160216299	0.0
6	1.0402980245780812E-4	0.0	0.0	0.002652759962674107	0.0
7	1.0402980245780812E-4	0.0	0.0	0.0030688791725053395	0.0
8	1.0402980245780812E-4	0.0	0.0	0.0031729089749631474	0.0
9	1.0402980245780812E-4	0.0	0.0	0.004681341110601365	0.0
10	1.0402980245780812E-4	0.0	0.0	0.004993430517974789	0.0
11	1.0402980245780812E-4	0.0	0.0	0.00577365403640835	0.0
12	1.0402980245780812E-4	0.0	0.0	0.005877683838866159	0.0
13	1.0402980245780812E-4	0.0	0.0	0.00603372854255287	0.0
14	1.0402980245780812E-4	0.0	0.0	0.006397832851155199	0.0
15	1.0402980245780812E-4	0.0	0.0	0.007074026567130951	0.0
16	1.0402980245780812E-4	0.0	0.0	0.007282086172046568	0.0
17	1.0402980245780812E-4	0.0	0.0	0.008270369295395746	0.0
18	1.0402980245780812E-4	0.0	0.0	0.008946563011371497	0.0
19	1.0402980245780812E-4	0.0	0.0	0.009726786529805059	0.0
20	1.0402980245780812E-4	0.0	0.0	0.011079173961756564	0.0
21	1.0402980245780812E-4	0.0	0.0	0.011755367677732317	0.0
22	1.0402980245780812E-4	0.0	0.0	0.013367829615828343	0.0
23	1.0402980245780812E-4	0.0	0.0	0.01383596372688848	0.0
24	1.0402980245780812E-4	0.0	0.0	0.01602058957850245	0.0
25	1.0402980245780812E-4	0.0	0.0	0.01830924523257423	0.0
26	1.0402980245780812E-4	0.0	0.0	0.02720379334271682	0.0
27	1.0402980245780812E-4	0.0	0.0	0.07724212832492253	0.0
28	1.0402980245780812E-4	0.0	0.0	0.10751480084014468	0.0
29	1.0402980245780812E-4	0.0	0.0	0.1244196437395385	0.0
30	1.0402980245780812E-4	0.0	0.0	0.15105127316873737	0.0
31	1.0402980245780812E-4	0.0	0.0	0.17503014263526215	0.0
32	1.0402980245780812E-4	0.0	0.0	0.21248087152007308	0.0
33	1.0402980245780812E-4	0.0	0.0	0.24540630399796934	0.0
34	1.0402980245780812E-4	0.0	0.0	0.2927918790175009	0.0
35	1.0402980245780812E-4	0.0	0.0	0.37154243947806165	0.0
36	1.0402980245780812E-4	0.0	0.0	0.5103702108580066	0.0
37	1.0402980245780812E-4	0.0	0.0	0.7732535216688877	0.0
38	1.0402980245780812E-4	0.0	0.0	1.1538465539607787	0.0
39	1.0402980245780812E-4	0.0	0.0	1.5822932953832614	0.0
40	1.0402980245780812E-4	0.0	0.0	2.0830927644151496	0.0
41	1.0402980245780812E-4	5.201490122890406E-5	0.0	2.8253974198528393	0.0
42	1.0402980245780812E-4	5.201490122890406E-5	0.0	3.8186739737199913	0.0
43	1.0402980245780812E-4	5.201490122890406E-5	0.0	4.913431599884735	0.0
44	1.0402980245780812E-4	5.201490122890406E-5	0.0	5.780103884160734	0.0
45	1.0402980245780812E-4	5.201490122890406E-5	0.0	6.50264287713144	0.0
46	1.0402980245780812E-4	5.201490122890406E-5	0.0	7.222789184645618	0.0
47	1.0402980245780812E-4	5.201490122890406E-5	0.0	7.9506857124429	0.0
48	1.0402980245780812E-4	5.201490122890406E-5	0.0	8.617204656790078	0.0
49	1.0402980245780812E-4	5.201490122890406E-5	0.0	9.2620333873248	0.0
50	1.0402980245780812E-4	5.201490122890406E-5	0.0	9.946289412991034	0.0
51	1.0402980245780812E-4	5.201490122890406E-5	0.0	10.758398065877913	0.0
52	1.0402980245780812E-4	5.201490122890406E-5	0.0	11.837291147167841	0.0
53	1.0402980245780812E-4	5.201490122890406E-5	0.0	13.083048031600093	0.0
54	1.0402980245780812E-4	5.201490122890406E-5	0.0	14.079341449738521	0.0
55	1.0402980245780812E-4	5.201490122890406E-5	0.0	14.86492250299866	1.0402980245780812E-4
56	1.0402980245780812E-4	5.201490122890406E-5	0.0	15.55739688305906	1.0402980245780812E-4
57	1.0402980245780812E-4	5.201490122890406E-5	0.0	16.225892393652934	1.0402980245780812E-4
58	1.0402980245780812E-4	5.201490122890406E-5	0.0	16.86115038236154	1.0402980245780812E-4
59	1.0402980245780812E-4	5.201490122890406E-5	0.0	17.44621399138425	1.0402980245780812E-4
60	1.0402980245780812E-4	5.201490122890406E-5	0.0	18.042512819072407	1.0402980245780812E-4
61	1.0402980245780812E-4	1.0402980245780812E-4	0.0	18.64853843329037	1.0402980245780812E-4
62	1.0402980245780812E-4	1.0402980245780812E-4	0.0	19.28442060081372	1.0402980245780812E-4
63	1.0402980245780812E-4	1.0402980245780812E-4	0.0	19.95936595915998	1.0402980245780812E-4
64	1.0402980245780812E-4	1.0402980245780812E-4	0.0	20.638680569209466	1.0402980245780812E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCG	45	9.205016E-5	38.88831	24
ACGAGCG	55	6.7022393E-6	38.18125	24
CGACGAT	125	1.05501385E-10	30.796337	22
CGATCGA	65	7.961984E-4	26.922676	25
CGTCGAA	80	8.680664E-5	26.24688	22
GTCGTTG	70	0.0012277056	24.997026	14
AATCGTC	140	3.2906246E-7	22.49908	28
AGAACCG	190	1.3573299E-8	20.261803	5
TCGGACG	125	7.837013E-5	19.59767	12
GACGATC	90	0.0052785845	19.444155	23
GACCGGT	95	0.007215108	18.41982	7
ATACCGT	95	0.007215108	18.41982	6
AACACCG	210	4.2799E-8	18.332108	5
CGATTGG	115	0.0010084542	18.2606	25
ACGATAT	115	0.0010084542	18.2606	24
AGCGTGT	135	1.4137919E-4	18.146935	8
CGACGCT	135	1.4143554E-4	18.14599	22
GACGAAG	7795	0.0	18.139091	70
CTCGTAT	215	5.592119E-8	17.908575	48
ACGAAGG	5650	0.0	17.778034	70
>>END_MODULE