FastQCFastQC Report
Wed 25 May 2016
SRR1779871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922526
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT155050.8064910435541575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22600.051.0933443
CGTATGC23250.049.81542641
CTCGTAT23000.049.74835239
TCGTATG23350.049.45222540
CCGTCTT23300.049.13464747
CGTCTTC23300.048.99076548
GCCGTCT23450.048.6710646
ATGCCGT23650.048.52901544
TGCCGTC23900.047.8874445
TCTCGTA22550.047.7928538
ATCTCGT23500.045.860837
GTATGCC26100.044.24174542
GATCTCG26900.039.6740336
CTGCTTG31700.037.0117754
AGATCTC29200.036.30935735
ACAGATC29250.036.12766333
GTCTTCT31800.035.89855249
CTTGAAA32350.034.96621357
CAGATCT30900.034.19851334
TGCTTGA34000.033.8890955
Produced by FastQC (version 0.11.5)