FastQCFastQC Report
Wed 25 May 2016
SRR1779870_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779870_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209024
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAT150.002222320370.00024443
ACGCGTG252.3864557E-456.00019534
CTCGATC200.00694402552.5001844
TCCGGTG200.00694402552.500188
GCCGTAT552.2664608E-950.9214554
TCGGTGG1050.050.0001745
GCGGTCG305.8682053E-446.6668348
GGACGCT305.8682053E-446.6668313
CGGTGGA305.875133E-446.65566346
TCGCCGT703.8016879E-1045.01092552
CCTGACG703.8198777E-1045.00015311
CTCGGTG1250.044.80015644
AGTGTCG404.5817545E-543.75015335
CGGTGGT1000.041.990146
TAAACGT350.001253391440.0001374
GGCTTAT350.001253391440.0001371
CTAAACG350.001253391440.0001373
GGTGGAC350.001254867539.9905747
GATATGC350.001254867539.9905726
TGGTCGC801.4097168E-939.3845649