##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779870_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209024
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.045506736068585	34.0	23.0	34.0	12.0	34.0
2	29.719850352112676	34.0	31.0	34.0	12.0	34.0
3	29.429725773116964	34.0	27.0	34.0	11.0	34.0
4	29.485327043784444	34.0	27.0	34.0	12.0	34.0
5	30.111451316595222	34.0	32.0	34.0	11.0	34.0
6	32.7149609614207	37.0	32.0	38.0	12.0	38.0
7	32.98542272657685	38.0	34.0	38.0	12.0	38.0
8	32.299511060930804	37.0	31.0	38.0	11.0	38.0
9	33.17630511328843	38.0	34.0	38.0	12.0	38.0
10-11	31.75377468616044	37.0	28.5	38.0	11.5	38.0
12-13	32.591955947642376	37.0	31.5	38.0	11.0	38.0
14-15	31.385391151255355	37.0	27.0	38.0	11.0	38.0
16-17	31.987774131200247	37.0	30.0	38.0	11.0	38.0
18-19	32.81524609614207	37.5	33.5	38.0	11.0	38.0
20-21	31.554075130128595	37.0	27.5	38.0	11.0	38.0
22-23	32.42713994565217	37.5	31.5	38.0	11.0	38.0
24-25	32.74438102801592	37.5	32.5	38.0	11.0	38.0
26-27	31.697639983925292	37.0	29.0	38.0	11.0	38.0
28-29	32.8402432256583	37.5	32.5	38.0	11.0	38.0
30-31	33.19917808481323	38.0	34.0	38.0	11.0	38.0
32-33	33.302453306797304	38.0	34.0	38.0	11.0	38.0
34-35	33.24862695192896	38.0	34.0	38.0	11.0	38.0
36-37	32.75375554960196	38.0	32.5	38.0	11.0	38.0
38-39	32.69539383037355	38.0	32.5	38.0	11.0	38.0
40-41	32.714470587109616	38.0	33.0	38.0	11.0	38.0
42-43	33.06667894595836	38.0	34.0	38.0	11.0	38.0
44-45	33.151140538885485	38.0	34.0	38.0	11.0	38.0
46-47	33.051274016380894	38.0	34.0	38.0	11.0	38.0
48-49	32.761345587109616	38.0	33.0	38.0	11.0	38.0
50-51	32.1773337033068	37.5	30.5	38.0	11.0	38.0
52-53	32.728234556797304	37.0	33.0	38.0	11.0	38.0
54-55	32.8576790225046	38.0	33.0	38.0	11.0	38.0
56-57	32.92194915416411	38.0	34.0	38.0	11.0	38.0
58-59	33.1198235609308	38.0	34.0	38.0	11.0	38.0
60-61	33.01595271356399	38.0	34.0	38.0	11.0	38.0
62-63	33.00986968003674	38.0	34.0	38.0	11.0	38.0
64-65	33.00092333894672	38.0	34.0	38.0	11.0	38.0
66-67	32.85747091243111	38.0	33.0	38.0	11.0	38.0
68-69	32.268000325321495	37.0	31.0	38.0	11.0	38.0
70-71	32.328235035211264	37.0	31.0	38.0	11.0	38.0
72-73	32.496636749846914	37.0	31.5	38.0	11.0	38.0
74-75	32.57603911512554	37.5	32.0	38.0	11.0	38.0
76	31.697953345070424	37.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	121.0
13	933.0
14	2340.0
15	3426.0
16	4302.0
17	4907.0
18	5026.0
19	4652.0
20	3662.0
21	2730.0
22	2244.0
23	1922.0
24	1808.0
25	1875.0
26	2076.0
27	2235.0
28	2654.0
29	3191.0
30	4001.0
31	4933.0
32	6391.0
33	8571.0
34	12173.0
35	18491.0
36	33510.0
37	70846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.45222774527205	19.580620314510846	10.172564741679144	31.79458719853796
2	21.67885027556644	21.07509185548071	31.493034292712796	25.753023576240047
3	17.87258879363135	23.42506123698714	30.754841549295776	27.947508420085732
4	14.799735915492956	21.944370024494795	31.328938303735455	31.926955756276794
5	14.114168707899571	33.539689222290264	34.66156996325781	17.684572106552356
6	28.483740868142437	38.34160203998641	16.784437927289602	16.39021916458155
7	26.860530206255113	30.588049547157354	19.72030447866879	22.831115767918742
8	23.510386864037816	36.97503516510856	18.690135589003606	20.82444238185002
9	23.550069374671068	18.274723697430744	19.74307449404335	38.43213243385484
10-11	23.0133480049756	27.084728734092433	24.688785762128028	25.213137498803945
12-13	22.978995184751955	23.65021157908082	26.24776639947183	27.123026836695395
14-15	24.28303173103374	25.685273116530517	22.3509430802904	27.680752072145342
16-17	24.581924998863762	26.86444347166706	23.150746207962264	25.40288532150691
18-19	21.654542540492827	27.076072213703572	25.63816121539481	25.631224030408788
20-21	24.02260027556644	26.264448101653397	24.881592544396817	24.831359078383343
22-23	24.56822313654196	25.917615539182854	24.220170318629798	25.293991005645395
24-25	21.6591912160465	28.548805722008925	24.11688016553638	25.675122896408197
26-27	23.23853537999316	28.286053960439855	24.54269496013606	23.932715699430926
28-29	21.748657933265072	27.950872223763913	26.019587954412792	24.280881888558223
30-31	22.330928505817514	27.345902862829146	25.406173453766073	24.91699517758726
32-33	21.34276446723821	28.223553276178816	25.586296310471525	24.84738594611145
34-35	21.668811627762334	28.321858987546467	25.625666811790428	24.383662572900768
36-37	23.375784444375327	26.742525199116685	25.554882994274724	24.326807362233264
38-39	23.02359296278044	26.034554527925437	26.865164614861985	24.076687894432137
40-41	22.05219596210889	26.989044110611427	26.373313558511146	24.585446368768537
42-43	20.629069528233735	26.900388952411937	26.79776866660607	25.67277285274826
44-45	20.10506448117236	25.456128641792798	28.084654413928224	26.35415246310663
46-47	20.76669138578571	25.616104107360716	27.771451809678734	25.845752697174845
48-49	20.844785394943628	25.888476654538845	27.747119343040378	25.519618607477152
50-51	22.942357052810863	25.790895107872707	26.430787558098643	24.83596028121778
52-53	18.693390877468314	27.308025397250706	27.364963468724728	26.633620256556252
54-55	19.368382272024803	26.548901211944326	26.86396845978287	27.218748056248
56-57	19.592569111331745	26.83708745529146	26.711245618861923	26.859097814514875
58-59	19.412858171587427	26.924981638360634	26.808471952745393	26.853688237306546
60-61	18.942625873029925	26.215662019280234	27.061475184296345	27.780236923393492
62-63	19.398132531417538	26.631881894454295	26.514417498606456	27.455568075521715
64-65	20.189885078373056	25.997458947161896	25.68521092891991	28.12744504554514
66-67	19.66098045314257	27.567528770007417	24.472091298418547	28.299399478431464
68-69	19.94177688154681	28.531241702487996	23.95496200188017	27.57201941408502
70-71	20.52850390385793	28.711296310471525	23.83027786282915	26.929921922841398
72-73	21.34648415633627	27.772700958322766	23.46335838783605	27.417456497504915
74-75	21.335495924806885	27.54553044493406	23.77845877081554	27.340514859443516
76	21.50628175563827	28.134419045249693	23.23149202461032	27.12780717450172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.0
11	2.0
12	1.0
13	1.5
14	4.0
15	18.0
16	30.5
17	31.0
18	38.5
19	64.0
20	133.5
21	185.0
22	216.0
23	279.5
24	431.0
25	550.0
26	652.5
27	846.5
28	1152.0
29	1366.0
30	1550.5
31	1972.5
32	2724.0
33	3238.0
34	3623.0
35	4592.0
36	5592.5
37	6009.0
38	6597.5
39	8035.0
40	9378.5
41	10162.5
42	10452.0
43	11022.0
44	12007.5
45	12725.5
46	13028.0
47	12906.5
48	12600.0
49	12018.0
50	11621.0
51	11242.0
52	10173.5
53	9045.0
54	8606.0
55	7957.0
56	6733.0
57	5669.5
58	5181.0
59	4561.5
60	3658.5
61	3039.5
62	2704.0
63	2462.5
64	2012.0
65	1697.5
66	1488.5
67	1385.0
68	1261.5
69	1040.0
70	877.0
71	812.0
72	800.0
73	695.5
74	571.0
75	539.0
76	491.5
77	428.5
78	371.0
79	329.0
80	292.0
81	242.5
82	208.5
83	187.0
84	161.5
85	123.0
86	90.5
87	71.0
88	62.0
89	40.0
90	26.5
91	27.0
92	28.0
93	25.5
94	21.5
95	14.5
96	9.0
97	8.5
98	9.0
99	9.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.7841396203306797E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0014352418860992039
7	0.005262553582363748
8	0.00478413962033068
9	0.006697795468462952
10-11	0.0019136558481322719
12-13	2.3920698101653398E-4
14-15	7.176209430496019E-4
16-17	2.3920698101653398E-4
18-19	0.002631276791181874
20-21	0.0
22-23	0.0019136558481322719
24-25	0.0031096907532149423
26-27	2.3920698101653398E-4
28-29	0.008611451316595223
30-31	0.0
32-33	0.0
34-35	0.003348897734231476
36-37	0.018418937538273117
38-39	0.010046693202694428
40-41	0.0019136558481322719
42-43	4.7841396203306797E-4
44-45	0.004544932639314146
46-47	0.0043057256582976116
48-49	2.3920698101653398E-4
50-51	0.0021528628291488058
52-53	0.011960349050826699
54-55	0.010046693202694428
56-57	0.015070039804041641
58-59	0.013634797917942438
60-61	0.025595146968769137
62-63	0.011721142069810165
64-65	0.026551974892835273
66-67	0.01865814451928965
68-69	2.3920698101653398E-4
70-71	0.0
72-73	0.0062193815064298845
74-75	0.007415416411512553
76	0.0028704837721984077
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	209024.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.11432469733885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0872908318247	41.89304225952159
2	12.132121026113886	14.101000411579884
3	6.770415666104486	11.803744030678375
4	3.726633146688027	8.662830748579745
5	1.9626883774502037	5.703015482341715
6	1.1151000721427196	3.888197259751679
7	0.5651002017379542	2.2988291627231807
8	0.3638440812467129	1.6915642461421128
9	0.2671978034706125	1.397521791837622
>10	1.0066509614146948	8.464567838638638
>50	0.002957831805997047	0.09568676820543125
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00239206981016534	0.0
2	0.0	0.0	0.0	0.0028704837721984077	0.0
3	0.0	0.0	0.0	0.003348897734231476	0.0
4	0.0	0.0	0.0	0.003348897734231476	0.0
5	0.0	0.0	0.0	0.0043057256582976116	0.0
6	0.0	0.0	0.0	0.005740967544396815	0.0
7	0.0	0.0	0.0	0.006219381506429884	0.0
8	0.0	0.0	0.0	0.006697795468462952	0.0
9	0.0	0.0	0.0	0.011003521126760563	0.0
10	0.0	0.0	0.0	0.01626607470912431	0.0
11	0.0	0.0	0.0	0.01865814451928965	0.0
12	0.0	0.0	0.0	0.01913655848132272	0.0
13	0.0	0.0	0.0	0.019614972443355787	0.0
14	0.0	0.0	0.0	0.019614972443355787	0.0
15	0.0	0.0	0.0	0.020571800367421924	0.0
16	0.0	0.0	0.0	0.020571800367421924	0.0
17	0.0	0.0	0.0	0.022485456215554193	0.0
18	0.0	0.0	0.0	0.024399112063686466	0.0
19	0.0	0.0	0.0	0.02583435394978567	0.0
20	0.0	0.0	0.0	0.02631276791181874	0.0
21	0.0	0.0	0.0	0.029183251684017145	0.0
22	0.0	0.0	0.0	0.04592774035517452	0.0
23	0.0	0.0	0.0	0.04592774035517452	0.0
24	0.0	0.0	0.0	0.048319810165339865	0.0
25	0.0	0.0	0.0	0.050711879975505206	0.0
26	0.0	0.0	0.0	0.05501760563380282	0.0
27	0.0	0.0	0.0	0.08180878750765462	0.0
28	0.0	0.0	0.0	0.0942475505205144	0.0
29	0.0	0.0	0.0	0.12199556031843234	0.0
30	0.0	0.0	0.0	0.17270744029393753	0.0
31	0.0	0.0	0.0	0.21432945499081446	0.0
32	0.0	0.0	0.0	0.2583435394978567	0.0
33	0.0	0.0	0.0	0.29518141457440294	0.0
34	0.0	0.0	0.0	0.3669435088793631	0.0
35	0.0	0.0	0.0	0.47315140845070425	0.0
36	0.0	0.0	0.0	0.6702579608083282	0.0
37	0.0	0.0	0.0	1.013280771586038	0.0
38	0.0	0.0	0.0	1.4395476117575015	0.0
39	0.0	0.0	0.0	1.9964214635639927	0.0
40	0.0	0.0	0.0	2.653762247397428	0.0
41	0.0	0.0	0.0	3.5010333741579913	0.0
42	0.0	0.0	0.0	4.379879822412737	0.0
43	0.0	0.0	0.0	5.289823178199633	0.0
44	0.0	0.0	0.0	6.172496938150643	0.0
45	0.0	0.0	0.0	7.05612752602572	0.0
46	0.0	0.0	0.0	7.9622435701163505	0.0
47	0.0	0.0	0.0	8.771241579914268	0.0
48	0.0	0.0	0.0	9.557754133496632	0.0
49	0.0	0.0	0.0	10.313648193508879	0.0
50	0.0	0.0	0.0	11.01069733619106	0.0
51	0.0	0.0	0.0	11.829742039191672	0.0
52	0.0	0.0	0.0	12.721027250459278	0.0
53	0.0	0.0	0.0	13.630013778322107	0.0
54	0.0	0.0	0.0	14.493550979791793	0.0
55	0.0	0.0	0.0	15.224567513778322	0.0
56	0.0	0.0	0.0	15.916832516840172	0.0
57	0.0	0.0	0.0	16.572738058787507	0.0
58	0.0	0.0	0.0	17.191327311696263	0.0
59	0.0	0.0	0.0	17.74246019595836	0.0
60	0.0	0.0	0.0	18.3318661971831	0.0
61	0.0	0.0	0.0	18.943757654623393	0.0
62	0.0	0.0	0.0	19.64032838334354	0.0
63	0.0	0.0	0.0	20.29336344151868	0.0
64	0.0	0.0	0.0	20.92965401102266	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGAT	15	0.0022223203	70.000244	43
ACGCGTG	25	2.3864557E-4	56.000195	34
CTCGATC	20	0.006944025	52.50018	44
TCCGGTG	20	0.006944025	52.50018	8
GCCGTAT	55	2.2664608E-9	50.92145	54
TCGGTGG	105	0.0	50.00017	45
GCGGTCG	30	5.8682053E-4	46.66683	48
GGACGCT	30	5.8682053E-4	46.66683	13
CGGTGGA	30	5.875133E-4	46.655663	46
TCGCCGT	70	3.8016879E-10	45.010925	52
CCTGACG	70	3.8198777E-10	45.000153	11
CTCGGTG	125	0.0	44.800156	44
AGTGTCG	40	4.5817545E-5	43.750153	35
CGGTGGT	100	0.0	41.9901	46
TAAACGT	35	0.0012533914	40.000137	4
GGCTTAT	35	0.0012533914	40.000137	1
CTAAACG	35	0.0012533914	40.000137	3
GGTGGAC	35	0.0012548675	39.99057	47
GATATGC	35	0.0012548675	39.99057	26
TGGTCGC	80	1.4097168E-9	39.38456	49
>>END_MODULE