FastQCFastQC Report
Wed 25 May 2016
SRR1779870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209024
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2304011.022657685241887No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG28681.372091243110839No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC16240.7769442743417023No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT6280.30044396815676666RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5740.27460961420698105No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT4210.2014122780159216No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT3890.18610303123086344No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT2680.1282149418248622No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2660.12725811390079608RNA PCR Primer, Index 32 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGCT352.9695912E-759.98400534
CCGATCT252.389644E-455.98507334
TCTCGTA35400.054.8582538
ATCTCGT35350.054.8368637
CGTCTTC37350.054.54971748
CGATCTC35450.054.48476435
CGTATGC38250.054.42992841
TATGCCG38000.054.419743
CTCGTAT37400.054.35716639
TCTTCTG37700.054.3348650
CCGTCTT37500.054.3315247
TCGTATG38450.054.23781240
ATGCCGT37950.054.21479444
CTGCTTG37950.054.17443554
TCGATCT35400.054.16634434
GCCGTCT37750.054.15717346
CTCGATC35350.054.1439833
ACTCGAT35900.054.09420832
GATCTCG35750.054.02755736
GTCTTCT38000.053.9980549