FastQCFastQC Report
Wed 25 May 2016
SRR1779869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193522
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTT150.002221057270.0082853
ATGATAC150.002223336669.9901827
CTGTGTA650.059.2377734
GCAGTGT252.384656E-456.00662231
TTACGCT252.3877059E-455.9921413
CCGTATG200.006940093452.50620724
GTGTACC200.006940093452.50620736
CGTAAGA200.006940093452.50620732
TACGCTT200.006947195652.4926314
TTAGACC200.006954303552.4790654
CGACGAT1150.048.6888222
GACGATC451.6771064E-646.6721823
GCCGTAT451.6771064E-646.6721854
CGACGAG604.9094524E-946.6601222
GATATGC305.8712746E-446.6601226
TAGATCC305.8712746E-446.6601239
TCGGTGG751.4551915E-1146.6601245
ACATCTT703.8198777E-1044.9820568
ACTGTGT404.5773755E-543.75517333
GTAGATC1300.040.3893938