##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779869_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193522
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.68569464970391	34.0	23.0	34.0	12.0	34.0
2	29.283363131840307	34.0	27.0	34.0	12.0	34.0
3	28.895179876189786	34.0	23.0	34.0	11.0	34.0
4	29.188257665795103	34.0	24.0	34.0	12.0	34.0
5	29.594196008722523	34.0	31.0	34.0	11.0	34.0
6	32.0933123882556	37.0	28.0	38.0	12.0	38.0
7	32.44175855975031	37.0	31.0	38.0	12.0	38.0
8	31.687332706358966	37.0	27.0	38.0	11.0	38.0
9	32.54719360072757	38.0	31.0	38.0	12.0	38.0
10-11	31.22950878969833	37.0	27.0	38.0	11.5	38.0
12-13	31.983929475718526	37.0	29.5	38.0	11.0	38.0
14-15	30.792685069397795	37.0	25.5	38.0	11.0	38.0
16-17	31.38227953410982	37.0	28.0	38.0	11.0	38.0
18-19	32.13736939469415	37.5	31.0	38.0	11.0	38.0
20-21	30.988768718802	37.0	27.0	38.0	11.0	38.0
22-23	31.81729467450729	37.0	28.5	38.0	11.0	38.0
24-25	32.123081613459966	37.5	29.5	38.0	11.0	38.0
26-27	31.059778733167292	37.0	25.5	38.0	11.0	38.0
28-29	32.13900228397805	37.5	29.5	38.0	11.0	38.0
30-31	32.45353758229039	38.0	31.0	38.0	11.0	38.0
32-33	32.58457694732382	38.0	32.5	38.0	11.0	38.0
34-35	32.54054319405546	38.0	32.0	38.0	11.0	38.0
36-37	32.061375450853134	38.0	29.0	38.0	11.0	38.0
38-39	32.013026942673186	37.5	29.0	38.0	11.0	38.0
40-41	32.029831233658186	37.5	29.0	38.0	11.0	38.0
42-43	32.374569816351624	38.0	32.5	38.0	11.0	38.0
44-45	32.418301278407625	38.0	31.0	38.0	11.0	38.0
46-47	32.36870226640899	38.0	31.5	38.0	11.0	38.0
48-49	32.07680005374066	37.5	29.0	38.0	11.0	38.0
50-51	31.4991887227292	37.5	28.5	38.0	11.0	38.0
52-53	32.03300916691642	37.0	29.5	38.0	11.0	38.0
54-55	32.154132346709936	37.5	31.0	38.0	11.0	38.0
56-57	32.221106644205825	38.0	31.0	38.0	11.0	38.0
58-59	32.39291915131096	38.0	31.5	38.0	11.0	38.0
60-61	32.30744049772119	38.0	31.0	38.0	11.0	38.0
62-63	32.28359049617098	38.0	31.0	38.0	11.0	38.0
64-65	32.26339640971052	38.0	31.0	38.0	11.0	38.0
66-67	32.12826965409617	38.0	31.0	38.0	11.0	38.0
68-69	31.575105156002934	37.0	27.0	38.0	11.0	38.0
70-71	31.62887682020649	37.0	27.0	38.0	11.0	38.0
72-73	31.74576017197011	37.0	27.5	38.0	11.0	38.0
74-75	31.79530234288608	37.0	28.5	38.0	11.0	38.0
76	30.98534016804291	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	91.0
13	835.0
14	2468.0
15	3901.0
16	5071.0
17	5893.0
18	6044.0
19	5309.0
20	4220.0
21	3061.0
22	2341.0
23	1853.0
24	1727.0
25	1688.0
26	1807.0
27	2005.0
28	2368.0
29	2832.0
30	3454.0
31	4392.0
32	5649.0
33	7441.0
34	10465.0
35	16149.0
36	29379.0
37	63078.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.85800064075402	17.867735968003636	11.560959477475429	30.71330391376691
2	21.12007937082089	25.666849247114023	28.111015801820983	25.10205558024411
3	18.617521522100848	24.170378561610566	30.314899597978524	26.89720031831006
4	13.625324252539764	23.408191316749516	28.9977366914356	33.96874773927512
5	14.110540403675035	32.18187079505172	34.07416211076777	19.633426690505473
6	25.002842112878387	37.974245289843836	19.28554449715272	17.737368100125053
7	25.829405928314525	30.846665839844555	18.90425399975195	24.419674232088965
8	23.205796022985655	36.59514655421886	18.70840051262971	21.49065691016578
9	23.032799169039485	19.99421221532626	18.882647498075045	38.090341117559205
10-11	22.797820363427785	27.276203567100822	23.782739117965466	26.143236951505926
12-13	22.99350978183359	23.690588150184475	24.719153377910523	28.59674869007141
14-15	25.116460530021367	25.165034195343644	21.477827930374303	28.240677344260686
16-17	25.490191012368136	25.62660996323406	21.909968659812993	26.973230364584815
18-19	21.862073063914778	26.106435918569833	24.695179455909216	27.336311561606173
20-21	24.54578807577433	25.275162513822718	23.59059951840101	26.588449892001943
22-23	25.299520715180794	25.138294986241554	22.814215768599738	26.74796852997791
24-25	21.900029971372174	28.06276418731074	22.51082586632768	27.526379974989407
26-27	24.15893923382794	27.71515162476327	23.006348164664725	25.119560976744065
28-29	22.34639036742287	27.37248720996331	24.666683892305308	25.614438530308508
30-31	22.936787222058587	26.591429353920248	24.47382971357114	25.997953710450027
32-33	21.711226630563967	27.308523061977453	24.669546614855157	26.310703692603425
34-35	21.990832846726644	27.84148162693717	24.653906375150502	25.513779151185684
36-37	24.17723546983369	25.548079010201867	24.239769708621456	26.034915811342984
38-39	24.07159541921614	24.767188275177773	25.803859351744666	25.35735695386142
40-41	22.092347282831962	26.551329452587463	25.334914569332806	26.021408695247768
42-43	20.418972617958776	26.461210928012978	25.910624686726507	27.20919176730174
44-45	20.3776529499615	24.788771697732944	27.164347246409765	27.669228105895787
46-47	21.28698033744154	25.11484897811539	26.44730382657675	27.150866857866312
48-49	21.05657510922559	25.996853062837978	26.623915172216005	26.322656655720422
50-51	23.535415127196814	25.4670084074432	24.99082777401702	26.006748691342967
52-53	18.777680735500073	26.941462834817393	26.242758435356922	28.03809799432561
54-55	19.729977648867585	25.7588403250604	25.897339827650807	28.613842198421207
56-57	19.91131920435342	26.32543164705457	25.483584233836172	28.27966491475584
58-59	19.676587475904267	26.464477852598723	25.693156036982103	28.16577863451491
60-61	18.94961533790052	26.01618242570995	25.78489416300783	29.24930807338169
62-63	19.750700251160193	25.971049394838296	25.26976465359531	29.008485700406194
64-65	20.380238159227638	25.59022968307457	24.642864526265736	29.386667631432058
66-67	19.716668346396464	27.96207428540418	23.265032935625	29.05622443257435
68-69	20.01167825880262	29.107284959849526	22.618875373342565	28.262161408005294
70-71	20.60789425409711	29.134897853199014	22.391167860769272	27.866040031934602
72-73	21.79888328781564	27.80205100909751	21.913603373390625	28.485462329696226
74-75	21.48061827220721	27.241908561447392	23.185207770261542	28.092265396083864
76	20.89488996026271	28.264632778871544	22.364497909787566	28.47597935107818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	6.5
15	10.0
16	17.5
17	28.0
18	39.5
19	53.5
20	76.0
21	96.0
22	158.0
23	222.0
24	283.0
25	342.0
26	415.0
27	587.0
28	808.0
29	930.0
30	1130.5
31	1492.0
32	1973.5
33	2294.0
34	2652.5
35	3440.5
36	4356.5
37	4843.0
38	5335.5
39	6609.5
40	7922.0
41	8922.0
42	9391.0
43	9834.5
44	10887.5
45	11678.5
46	11860.0
47	12126.5
48	11968.0
49	11075.0
50	10607.0
51	10219.5
52	9215.5
53	8278.5
54	7958.0
55	7281.5
56	6172.0
57	5313.0
58	4887.0
59	4489.5
60	3760.5
61	3193.0
62	2957.0
63	2783.0
64	2490.5
65	2241.0
66	1969.5
67	1829.0
68	1665.0
69	1435.0
70	1337.0
71	1305.0
72	1188.5
73	1010.0
74	882.0
75	816.0
76	780.5
77	707.0
78	651.5
79	634.0
80	545.5
81	437.0
82	369.0
83	321.0
84	285.5
85	215.0
86	151.0
87	122.0
88	116.0
89	87.0
90	51.5
91	34.5
92	30.0
93	24.0
94	15.5
95	12.0
96	11.0
97	10.0
98	9.5
99	9.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.002066948460640134
7	0.005167371151600334
8	0.005167371151600334
9	0.005684108266760368
10-11	0.0018085799030601172
12-13	0.0
14-15	7.751056727400503E-4
16-17	2.5836855758001675E-4
18-19	0.0033587912485402173
20-21	0.0
22-23	0.0023253170182201506
24-25	0.002066948460640134
26-27	7.751056727400503E-4
28-29	0.006200845381920402
30-31	5.167371151600335E-4
32-33	0.0
34-35	0.002583685575800167
36-37	0.014468639224480937
38-39	0.009301268072880602
40-41	0.0012918427879000836
42-43	5.167371151600335E-4
44-45	0.005684108266760368
46-47	0.0036171598061202344
48-49	2.5836855758001675E-4
50-51	0.0015502113454801006
52-53	0.010851479418360703
54-55	0.010076373745620653
56-57	0.009818005188040636
58-59	0.011884953648680769
60-61	0.020927853163981356
62-63	0.010334742303200669
64-65	0.019636010376081273
66-67	0.01989437893366129
68-69	0.0
70-71	7.751056727400503E-4
72-73	0.0038755283637002514
74-75	0.007751056727400503
76	5.167371151600335E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	193522.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.40154681877399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46632189561002	45.58282575218357
2	10.360173478270967	12.515410067968089
3	5.828197502969501	10.56096433034022
4	3.2999163509690845	7.97280207884388
5	1.8140485333002134	5.478566870783055
6	1.0148320128002108	3.6778454000605545
7	0.63771573644318	2.6963311838289172
8	0.3471022687440444	1.6772411149156854
9	0.26081836245525025	1.4178449277933085
>10	0.9688904601380691	8.351283086267317
>50	0.001983398299445715	0.06888518701544208
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0031004226909602008	0.0
2	0.0	0.0	0.0	0.0031004226909602008	0.0
3	0.0	0.0	0.0	0.0031004226909602008	0.0
4	0.0	0.0	0.0	0.007751056727400502	0.0
5	0.0	0.0	0.0	0.01033474230320067	0.0
6	0.0	0.0	0.0	0.01033474230320067	0.0
7	0.0	0.0	0.0	0.01033474230320067	0.0
8	0.0	0.0	0.0	0.01033474230320067	0.0
9	0.0	0.0	0.0	0.012918427879000837	0.0
10	0.0	0.0	0.0	0.014468639224480937	0.0
11	0.0	0.0	0.0	0.016535587685121072	0.0
12	0.0	0.0	0.0	0.016535587685121072	0.0
13	0.0	0.0	0.0	0.01808579903060117	0.0
14	0.0	0.0	0.0	0.019636010376081273	0.0
15	0.0	0.0	0.0	0.022736433067041473	0.0
16	0.0	0.0	0.0	0.022736433067041473	0.0
17	0.0	0.0	0.0	0.024803381527681606	0.0
18	0.0	0.0	0.0	0.027387067103481776	0.0
19	0.0	0.0	0.0	0.02842054133380184	0.0
20	0.0	0.0	0.0	0.031004226909602008	0.0
21	0.0	0.0	0.0	0.031520964024762045	0.0
22	0.0	0.0	0.0	0.04185570632796271	0.0
23	0.0	0.0	0.0	0.048056551709883114	0.0
24	0.0	0.0	0.0	0.05684108266760368	0.0
25	0.0	0.0	0.0	0.05787455689792375	0.0
26	0.0	0.0	0.0	0.06614235074048429	0.0
27	0.0	0.0	0.0	0.0893955209226858	0.0
28	0.0	0.0	0.0	0.12763406744452827	0.0
29	0.0	0.0	0.0	0.15812155723897026	0.0
30	0.0	0.0	0.0	0.18757557280309214	0.0
31	0.0	0.0	0.0	0.23046475336137492	0.0
32	0.0	0.0	0.0	0.2749041452651378	0.0
33	0.0	0.0	0.0	0.33071175370242145	0.0
34	0.0	0.0	0.0	0.4237244344312275	0.0
35	0.0	0.0	0.0	0.5100195326629531	0.0
36	0.0	0.0	0.0	0.7239486983392069	0.0
37	0.0	0.0	0.0	1.1326877564307933	0.0
38	0.0	0.0	0.0	1.6142867477599445	0.0
39	0.0	0.0	0.0	2.191998842508862	0.0
40	0.0	0.0	0.0	2.9112969068116286	0.0
41	0.0	0.0	0.0	3.6466138216843564	0.0
42	0.0	0.0	0.0	4.490962267855851	0.0
43	0.0	0.0	0.0	5.253149512716901	0.0
44	0.0	0.0	0.0	5.994150535856388	0.0
45	0.0	0.0	0.0	6.832814873761123	0.0
46	0.0	0.0	0.0	7.650292989944296	0.0
47	0.0	0.0	0.0	8.522028503219273	0.0
48	0.0	0.0	0.0	9.301784809995763	0.0
49	0.0	0.0	0.0	10.02004940006821	0.0
50	0.0	0.0	0.0	10.773452113971539	0.0
51	0.0	0.0	0.0	11.56974400843315	0.0
52	0.0	0.0	0.0	12.400140552495323	0.0
53	0.0	0.0	0.0	13.27084259153998	0.0
54	0.0	0.0	0.0	14.011843614679467	0.0
55	0.0	0.0	0.0	14.693936606690713	0.0
56	0.0	0.0	0.0	15.44940626905468	0.0
57	0.0	0.0	0.0	16.03952005456744	0.0
58	0.0	0.0	0.0	16.66167154122012	0.0
59	0.0	0.0	0.0	17.230599105011315	0.0
60	0.0	0.0	0.0	17.8708363906946	0.0
61	0.0	0.0	0.0	18.460950176207355	0.0
62	0.0	0.0	0.0	19.019542997695353	0.0
63	0.0	0.0	0.0	19.61689110282035	0.0
64	0.0	0.0	0.0	20.21320573371503	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATTT	15	0.0022210572	70.00828	53
ATGATAC	15	0.0022233366	69.99018	27
CTGTGTA	65	0.0	59.23777	34
GCAGTGT	25	2.384656E-4	56.006622	31
TTACGCT	25	2.3877059E-4	55.99214	13
CCGTATG	20	0.0069400934	52.506207	24
GTGTACC	20	0.0069400934	52.506207	36
CGTAAGA	20	0.0069400934	52.506207	32
TACGCTT	20	0.0069471956	52.49263	14
TTAGACC	20	0.0069543035	52.479065	4
CGACGAT	115	0.0	48.68882	22
GACGATC	45	1.6771064E-6	46.67218	23
GCCGTAT	45	1.6771064E-6	46.67218	54
CGACGAG	60	4.9094524E-9	46.66012	22
GATATGC	30	5.8712746E-4	46.66012	26
TAGATCC	30	5.8712746E-4	46.66012	39
TCGGTGG	75	1.4551915E-11	46.66012	45
ACATCTT	70	3.8198777E-10	44.982056	8
ACTGTGT	40	4.5773755E-5	43.755173	33
GTAGATC	130	0.0	40.38939	38
>>END_MODULE