FastQCFastQC Report
Wed 25 May 2016
SRR1779869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193522
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2718014.04491479004971No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG34191.7667241967321545No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC19361.0004030549498248No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT7420.38341893944874483RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6650.3436301815814223No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT3390.17517388203925136No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT3090.15967176858445034No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2920.1508872376267298No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC2110.10903153129876707No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2080.10748131995328696No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2010.10386416014716672No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA150.002212511470.0763448
AAACGAC150.002219334270.0219870
TCTTCGC150.002226172769.967729
CTGCAAG150.002226172769.967728
ATCGTAT452.6977432E-854.4193239
TCTTTAT1200.052.5029071
CGAAACG200.006956029752.47577716
ATATCGT407.475519E-752.47577737
AATCTCG38400.052.2024636
TCTCGTA38850.051.95838
CCGTCTT40200.051.933747
ATCTCGT38950.051.91441737
TCGTATG40500.051.91430740
TATGCCG40250.051.88908843
CGTCTTC40150.051.83729648
CGTATGC40500.051.8279341
ATGCCGT40300.051.73790444
AGTTAAT38550.051.72709732
TGCCGTC40350.051.7138845
CTCGTAT40200.051.69255439
Produced by FastQC (version 0.11.5)