##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779869_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193522
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.5419797232356	34.0	34.0	34.0	34.0	34.0
2	33.62838333626151	34.0	34.0	34.0	34.0	34.0
3	33.747708270894265	34.0	34.0	34.0	34.0	34.0
4	33.53501410692324	34.0	34.0	34.0	34.0	34.0
5	33.595405173572	34.0	34.0	34.0	34.0	34.0
6	37.27148851293393	38.0	38.0	38.0	36.0	38.0
7	37.33282520850343	38.0	38.0	38.0	37.0	38.0
8	37.23234567646056	38.0	38.0	38.0	36.0	38.0
9	37.144965430286994	38.0	38.0	38.0	36.0	38.0
10-11	37.103218238753215	38.0	38.0	38.0	35.5	38.0
12-13	37.2674347102655	38.0	38.0	38.0	36.5	38.0
14-15	37.28796725953639	38.0	38.0	38.0	36.0	38.0
16-17	37.18794245615486	38.0	38.0	38.0	36.0	38.0
18-19	37.04344725664266	38.0	38.0	38.0	35.5	38.0
20-21	37.06827389134052	38.0	38.0	38.0	35.5	38.0
22-23	37.078084145471834	38.0	38.0	38.0	36.0	38.0
24-25	37.25040305494983	38.0	38.0	38.0	36.0	38.0
26-27	37.1385165510898	38.0	38.0	38.0	36.0	38.0
28-29	36.626959725509245	38.0	37.0	38.0	34.0	38.0
30-31	36.85901344549974	38.0	37.0	38.0	35.0	38.0
32-33	36.900047539814594	38.0	37.5	38.0	35.5	38.0
34-35	37.002873058360294	38.0	38.0	38.0	36.0	38.0
36-37	36.958599022333374	38.0	38.0	38.0	35.5	38.0
38-39	36.23048800653156	38.0	37.0	38.0	34.0	38.0
40-41	36.716735048211575	38.0	37.0	38.0	35.0	38.0
42-43	37.12088806440612	38.0	38.0	38.0	36.0	38.0
44-45	36.885160343526834	38.0	37.5	38.0	35.0	38.0
46-47	37.041462986120436	38.0	38.0	38.0	36.0	38.0
48-49	36.67867477599446	38.0	37.0	38.0	34.5	38.0
50-51	36.826244561341866	38.0	37.0	38.0	34.5	38.0
52-53	36.73884622936927	38.0	37.0	38.0	34.5	38.0
54-55	36.749439340230055	38.0	37.0	38.0	34.5	38.0
56-57	36.61032079040109	38.0	37.0	38.0	34.0	38.0
58-59	36.685748907101	38.0	37.0	38.0	34.5	38.0
60-61	36.34333822511135	38.0	37.0	38.0	34.0	38.0
62-63	36.18586517295191	38.0	37.0	38.0	34.0	38.0
64-65	36.56932028399872	38.0	37.0	38.0	34.0	38.0
66-67	36.32196597802834	38.0	37.0	38.0	33.5	38.0
68-69	36.36665340374738	38.0	37.0	38.0	34.0	38.0
70-71	35.21841702752142	38.0	36.5	38.0	28.5	38.0
72-73	33.23123469166296	38.0	34.0	38.0	20.0	38.0
74-75	32.92403964407148	38.0	34.0	38.0	11.0	38.0
76	32.690092082553925	38.0	32.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	9.0
23	34.0
24	48.0
25	107.0
26	218.0
27	414.0
28	741.0
29	1154.0
30	1945.0
31	3065.0
32	4624.0
33	7353.0
34	12588.0
35	25801.0
36	33325.0
37	102094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.57167156971724	13.827119643051025	10.15681144883852	38.44439733839321
2	19.607589834747472	18.02430731389713	39.87453622843915	22.49356662291626
3	17.843966060706276	17.30914314651564	41.225287047467475	23.62160374531061
4	12.251837000444393	29.800229431279128	31.181467740101898	26.766465828174574
5	11.950579262306094	28.339930343836876	44.74271659036182	14.96677380349521
6	23.039241016525253	45.549343227126634	18.118353468856256	13.29306228749186
7	23.811246266574344	26.204772583995616	18.35450233048439	31.62947881894565
8	21.29008588170854	44.35051311995535	15.963042961523755	18.39635803681235
9	20.99347877760668	17.528756420458656	16.433790473434545	45.04397432850012
10-11	20.752679281942104	29.63203150029454	28.485391841754428	21.12989737600893
12-13	21.356744969564183	18.873306394105064	29.875931418650076	29.89401721768068
14-15	19.71377931191286	18.565589442027264	20.109599942125442	41.61103130393444
16-17	28.830313866123745	28.631111708229557	19.263701284608466	23.274873141038228
18-19	29.072146836018643	22.71524684531991	20.18375171815091	28.028854600510538
20-21	19.806533624084082	20.722708529262825	20.429460216409502	39.04129763024359
22-23	19.80575851841134	29.65605977614948	20.17109165882949	30.36709004660969
24-25	28.66466343358972	29.74826956317289	19.975661493226816	21.611405510010567
26-27	28.80215169334753	30.66137183369333	20.697388410619983	19.839088062339165
28-29	19.78018003121092	30.797273694980415	20.97694318992156	28.445603083887107
30-31	19.225204369529045	30.560349727679544	29.9221793904569	20.292266512334514
32-33	26.87498062235818	32.057595518855734	21.811731999462594	19.255691859323488
34-35	21.17588697925817	21.473785926147933	38.13674931015595	19.213577784437945
36-37	19.381000609749798	37.32262998522132	23.717200111615217	19.579169293413667
38-39	19.021868314713572	23.01727968913095	29.918820599208356	28.042031396947113
40-41	18.94254916753651	19.937784851334733	31.388937691838652	29.73072828929011
42-43	28.00419592705701	19.56712708181541	30.676257357082694	21.752419634044884
44-45	19.45566912289042	28.311251433945493	32.23742003493143	19.995659408232655
46-47	27.200525004909004	19.787155982265585	24.469052614173066	28.54326639865235
48-49	26.408625375926253	19.193425036946703	23.350833496966754	31.047116090160294
50-51	18.959712033158567	20.084291801575755	32.02209881934628	28.93389734591939
52-53	16.951815822935295	19.636525280927916	40.91287357985382	22.498785316282966
54-55	16.164195887347972	19.830944410696514	33.99180571531969	30.013053986635818
56-57	25.132546627202107	19.824994506843826	25.03793509028164	30.004523775672425
58-59	17.129255111561395	20.013594646913937	39.75426254209559	23.102887699429075
60-61	25.453384781985044	28.503345626907677	24.54493529720384	21.498334293903437
62-63	17.030729072442043	39.54927247822603	21.36071123975913	22.05928720957279
64-65	16.257147951286697	40.94868462192409	20.421241398556585	22.37292602823263
66-67	16.149734555360844	40.71564081489178	20.09754508940341	23.037079540343967
68-69	16.289934162248198	40.94420844831685	19.970181803148222	22.79567558628673
70-71	16.874210244788586	39.38401031559199	19.87146855062315	23.870310888996272
72-73	17.148357753831046	35.98444323849394	20.373930795668915	26.4932682120061
74-75	17.685287110394476	34.79382109283348	20.78921120224086	26.73168059453118
76	17.800183981229782	35.134730074729454	20.82140383879937	26.2436821052414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	6.0
14	11.0
15	8.5
16	20.0
17	33.0
18	42.0
19	64.0
20	97.5
21	118.0
22	165.0
23	227.0
24	286.5
25	331.0
26	423.5
27	623.5
28	838.5
29	946.0
30	1079.5
31	1373.5
32	1795.0
33	2056.0
34	2426.5
35	3108.5
36	8106.0
37	12792.0
38	14982.5
39	16611.5
40	14377.0
41	11513.5
42	10323.0
43	9654.0
44	9292.5
45	9428.0
46	9256.0
47	9474.0
48	9318.0
49	8513.0
50	8082.0
51	7627.0
52	6660.5
53	5782.5
54	5416.0
55	5001.5
56	4333.5
57	3730.5
58	3381.0
59	3175.5
60	2753.5
61	2395.0
62	2253.0
63	2120.0
64	1913.0
65	1784.5
66	1641.0
67	1552.0
68	1450.5
69	1252.0
70	1160.0
71	1165.0
72	1122.5
73	1018.0
74	866.5
75	777.0
76	745.0
77	656.0
78	555.0
79	511.0
80	471.5
81	398.5
82	317.0
83	269.0
84	225.0
85	170.0
86	126.5
87	94.0
88	81.0
89	46.5
90	24.5
91	23.5
92	23.0
93	15.5
94	6.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054257397091803515
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.751056727400503E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.167371151600335E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014210270666900922
52-53	0.028937278448961875
54-55	0.04883165738262317
56-57	0.051415342958423337
58-59	0.032812806812662125
60-61	0.031262595467182026
62-63	0.02945401556412191
64-65	0.011626585091100754
66-67	0.03772180940668245
68-69	0.008267793842560536
70-71	0.014727007782060954
72-73	0.01911927326092124
74-75	0.012918427879000837
76	0.012401690763840805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	193522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.868376722026436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.85459739118276	10.051053626977811
2	16.997263338726754	8.79383222579345
3	13.942989552745649	10.8204751914511
4	10.26148099318831	10.617914242308368
5	6.861628812848325	8.874959952873574
6	4.126965102574859	6.405473279523775
7	2.5908391762050296	4.691456268537944
8	1.4382453406843652	2.976405783321793
9	1.1186352649767284	2.604355060406569
>10	3.7354427598330036	14.81226940606236
>50	0.03795369649028185	0.6247351722284805
>100	0.02397075567807275	1.187978627752917
>500	0.003995125946345458	0.7270491210301672
>1k	0.003995125946345458	2.767127251681979
>5k	0.0	0.0
>10k+	0.001997562973172729	14.04491479004971
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	27180	14.04491479004971	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	3419	1.7667241967321545	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	1936	1.0004030549498248	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	742	0.38341893944874483	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	665	0.3436301815814223	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT	339	0.17517388203925136	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT	309	0.15967176858445034	No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	292	0.1508872376267298	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC	211	0.10903153129876707	No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	208	0.10748131995328696	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT	201	0.10386416014716672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0031004226909602008	0.0
2	0.0	0.0	0.0	0.0031004226909602008	0.0
3	0.0	0.0	0.0	0.0031004226909602008	0.0
4	0.0	0.0	0.0	0.007751056727400502	0.0
5	0.0	0.0	0.0	0.010851479418360703	0.0
6	0.0	0.0	0.0	0.010851479418360703	0.0
7	0.0	0.0	0.0	0.010851479418360703	0.0
8	0.0	0.0	0.0	0.010851479418360703	0.0
9	0.0	0.0	0.0	0.013435164994160871	0.0
10	0.0	0.0	0.0	0.014985376339640972	0.0
11	0.0	0.0	0.0	0.017052324800281106	0.0
12	0.0	0.0	0.0	0.017052324800281106	0.0
13	0.0	0.0	0.0	0.01911927326092124	0.0
14	0.0	0.0	0.0	0.02066948460640134	0.0
15	0.0	0.0	0.0	0.024286644412521572	0.0
16	0.0	0.0	0.0	0.024286644412521572	0.0
17	0.0	0.0	0.0	0.026353592873161708	0.0
18	0.0	0.0	0.0	0.028937278448961875	0.0
19	0.0	0.0	0.0	0.029970752679281943	0.0
20	0.0	0.0	0.0	0.033071175370242144	0.0
21	0.0	0.0	0.0	0.033587912485402174	0.0
22	0.0	0.0	0.0	0.03617159806120234	0.0
23	0.0	0.0	0.0	0.04340591767344281	0.0
24	0.0	0.0	0.0	0.04960676305536321	0.0
25	0.0	0.0	0.0	0.05322392286148345	0.0
26	0.0	0.0	0.0	0.060974979588883954	0.0
27	0.0	0.0	0.0	0.08732857246204566	0.0
28	0.0	0.0	0.0	0.1281508045596883	0.0
29	0.0	0.0	0.0	0.16122197992993045	0.0
30	0.0	0.0	0.0	0.19170946972437242	0.0
31	0.0	0.0	0.0	0.23563212451297527	0.0
32	0.0	0.0	0.0	0.28213846487737826	0.0
33	0.0	0.0	0.0	0.3441469186965823	0.0
34	0.0	0.0	0.0	0.44594413038310887	0.0
35	0.0	0.0	0.0	0.5430907080331951	0.0
36	0.0	0.0	0.0	0.77252198716425	0.0
37	0.0	0.0	0.0	1.2153656948563987	0.0
38	0.0	0.0	0.0	1.7041990057977905	0.0
39	0.0	0.0	0.0	2.2927625799650686	0.0
40	0.0	0.0	0.0	3.045648556753237	0.0
41	0.0	0.0	0.0	3.8031851675778463	0.0
42	0.0	0.0	0.0	4.681638263349903	0.0
43	0.0	0.0	0.0	5.476896683581195	0.0
44	0.0	0.0	0.0	6.244768036709004	0.0
45	0.0	0.0	0.0	7.1020349107595	0.0
46	0.0	0.0	0.0	7.951033990967435	0.0
47	0.0	0.0	0.0	8.848606360000414	0.0
48	0.0	0.0	0.0	9.655232996765225	0.0
49	0.0	0.0	0.0	10.417420241626274	0.0
50	0.0	0.0	0.0	11.197693285517925	0.0
51	0.0	0.0	0.0	12.005353396513058	0.0
52	0.0	0.0	0.0	12.836783414805552	0.0
53	0.0	0.0	0.0	13.706451979619889	0.0
54	0.0	0.0	0.0	14.443319105838096	0.0
55	0.0	0.0	0.0	15.120761463812899	0.0
56	0.0	0.0	0.0	15.88243197155879	0.0
57	0.0	0.0	0.0	16.48184702514443	0.0
58	0.0	0.0	0.0	17.099864614875827	0.0
59	0.0	0.0	0.0	17.683777555006667	0.0
60	0.0	0.0	0.0	18.330732423187026	0.0
61	0.0	0.0	0.0	18.928597265427186	0.0
62	0.0	0.0	0.0	19.493390932297103	0.0
63	0.0	0.0	0.0	20.100040305494982	0.0
64	0.0	0.0	0.0	20.715474209650584	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	15	0.0022125114	70.07634	48
AAACGAC	15	0.0022193342	70.02198	70
TCTTCGC	15	0.0022261727	69.9677	29
CTGCAAG	15	0.0022261727	69.9677	28
ATCGTAT	45	2.6977432E-8	54.41932	39
TCTTTAT	120	0.0	52.502907	1
CGAAACG	20	0.0069560297	52.475777	16
ATATCGT	40	7.475519E-7	52.475777	37
AATCTCG	3840	0.0	52.20246	36
TCTCGTA	3885	0.0	51.958	38
CCGTCTT	4020	0.0	51.9337	47
ATCTCGT	3895	0.0	51.914417	37
TCGTATG	4050	0.0	51.914307	40
TATGCCG	4025	0.0	51.889088	43
CGTCTTC	4015	0.0	51.837296	48
CGTATGC	4050	0.0	51.82793	41
ATGCCGT	4030	0.0	51.737904	44
AGTTAAT	3855	0.0	51.727097	32
TGCCGTC	4035	0.0	51.71388	45
CTCGTAT	4020	0.0	51.692554	39
>>END_MODULE