FastQCFastQC Report
Wed 25 May 2016
SRR1779868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113186
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC650.064.6153949
ACGATAT700.055.00000424
CGGTGGT850.053.5294146
GTGGTCG1050.053.33333248
AGATCCC200.00693593152.540
GCGAACT200.00693593152.528
TCCGAAA200.00693593152.520
GATCCCG200.00693593152.541
CGAGACG200.00693593152.569
GCCGTCT200.00693593152.555
CGATATG707.2759576E-1250.00000425
ATCTCGG1050.049.99999642
ATATGCA800.048.12527
GGTGGTC1250.047.60000247
AGATCTC1400.047.50000440
TCTCGGG305.856837E-446.66666445
TCTCGGC604.8730726E-946.66666443
GACGCTT305.856837E-446.66666414
TGTAGAT1600.045.937537
TCGGTGG851.8189894E-1245.29411745
Produced by FastQC (version 0.11.5)