##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779868_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	113186
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.29469192302935	34.0	23.0	34.0	12.0	34.0
2	29.03133779796088	34.0	23.0	34.0	12.0	34.0
3	28.7078437262559	34.0	23.0	34.0	11.0	34.0
4	28.86017705369922	34.0	23.0	34.0	12.0	34.0
5	29.103546374993375	34.0	27.0	34.0	11.0	34.0
6	31.638055943314544	37.0	27.0	38.0	12.0	38.0
7	31.953943067163785	37.0	28.0	38.0	12.0	38.0
8	31.30033749757037	37.0	27.0	38.0	11.0	38.0
9	32.125660417366106	37.0	31.0	38.0	12.0	38.0
10-11	30.78242450479741	36.5	25.5	38.0	11.0	38.0
12-13	31.547991801106143	37.0	27.0	38.0	11.0	38.0
14-15	30.39620182708109	36.5	24.5	38.0	11.0	38.0
16-17	30.93060537522308	37.0	25.5	38.0	11.0	38.0
18-19	31.686776633152512	37.0	28.0	38.0	11.0	38.0
20-21	30.597286766914635	36.5	25.0	38.0	11.0	38.0
22-23	31.385038785715548	37.0	28.0	38.0	11.0	38.0
24-25	31.71797307087449	37.0	29.0	38.0	11.0	38.0
26-27	30.642751753750467	37.0	24.5	38.0	11.0	38.0
28-29	31.709345678794197	37.0	29.0	38.0	11.0	38.0
30-31	32.05852313890411	38.0	31.0	38.0	11.0	38.0
32-33	32.19816054989133	38.0	31.0	38.0	11.0	38.0
34-35	32.154944074355484	38.0	31.0	38.0	11.0	38.0
36-37	31.70544943720955	37.0	27.0	38.0	11.0	38.0
38-39	31.619869065078717	37.0	28.0	38.0	11.0	38.0
40-41	31.630426907921475	37.0	27.0	38.0	11.0	38.0
42-43	31.932725778806564	37.5	29.0	38.0	11.0	38.0
44-45	32.012625236336646	37.0	30.5	38.0	11.0	38.0
46-47	31.904983831922678	37.0	29.0	38.0	11.0	38.0
48-49	31.624454437828	37.0	27.0	38.0	11.0	38.0
50-51	31.054388351916316	37.0	25.5	38.0	11.0	38.0
52-53	31.581525100277418	37.0	27.0	38.0	11.0	38.0
54-55	31.706200413478697	37.0	28.0	38.0	11.0	38.0
56-57	31.735841888572793	37.0	27.0	38.0	11.0	38.0
58-59	31.90930415422402	37.0	29.0	38.0	11.0	38.0
60-61	31.838018836251834	37.0	27.5	38.0	11.0	38.0
62-63	31.82787182160338	37.0	27.0	38.0	11.0	38.0
64-65	31.8228623681374	37.0	29.0	38.0	11.0	38.0
66-67	31.714023819200257	37.0	27.0	38.0	11.0	38.0
68-69	31.142274662944182	37.0	25.5	38.0	11.0	38.0
70-71	31.177822345519765	37.0	25.5	38.0	11.0	38.0
72-73	31.287009877546694	37.0	26.0	38.0	11.0	38.0
74-75	31.355445903203574	37.0	26.0	38.0	11.0	38.0
76	30.502623999434558	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	31.0
13	432.0
14	1366.0
15	2355.0
16	3202.0
17	3612.0
18	3801.0
19	3363.0
20	2648.0
21	2042.0
22	1604.0
23	1319.0
24	1227.0
25	1200.0
26	1281.0
27	1407.0
28	1571.0
29	1820.0
30	2183.0
31	2727.0
32	3553.0
33	4655.0
34	6275.0
35	9470.0
36	16748.0
37	33293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.4272209215002	18.483014533727967	9.567522198171135	34.5222423466007
2	19.736539854752355	20.894810312229428	32.4642623646034	26.90438746841482
3	17.39791140247027	20.404466983549202	34.55374339582634	27.64387821815419
4	12.820490166628382	25.4413089958122	29.12020921315357	32.617991624405846
5	17.046277808209496	32.1214637852738	32.47574788401392	18.356510522502784
6	26.477240599378003	36.8903731976251	16.1939850155499	20.43840118744699
7	25.614087792464836	31.801795433660846	17.409168021488654	25.174948752385667
8	22.48080508212509	39.53490426838427	16.130799339111686	21.85349131037895
9	22.810412469515427	19.15138020004948	17.78443431237409	40.253773018061004
10-11	22.266251408124045	27.360236785722176	23.28098425109889	27.09252755505489
12-13	22.637075256657184	23.378774760129346	25.068913116463165	28.915236866750305
14-15	24.958254185625304	25.30061403896276	21.29036533109511	28.450766444316827
16-17	25.702055475303816	26.093890118433897	21.757645634820715	26.446408771441572
18-19	22.069276270160668	25.873242360923975	24.464038803899825	27.59344256501553
20-21	24.95405792235789	24.79370240135706	23.687558531973917	26.564681144311137
22-23	25.740387333898784	24.69341956460277	22.675908255583828	26.890284845914618
24-25	21.919369516623522	28.289142362368906	22.38366185734607	27.407826263661505
26-27	23.930291116314	27.982506515881077	23.276935989751294	24.81026637805363
28-29	22.57770944883281	27.21244411458057	24.66247857357437	25.547367863012244
30-31	23.240167689324164	26.562589731016782	24.286679830897068	25.91056274876199
32-33	21.550021866758552	27.41075491118562	25.164884194530217	25.87433902752561
34-35	21.706190030216817	28.727182767577887	24.117792581859305	25.448834620345988
36-37	24.001131086771142	25.604534951023062	24.588324180072547	25.80600978213325
38-39	24.118826928684403	24.854868210615606	26.015922525690755	25.010382335009236
40-41	22.237045544904362	27.026549454433006	24.884481159164203	25.85192384149843
42-43	20.393603392675708	26.230949330741705	25.86561823563193	27.509829040950656
44-45	19.84591189411744	24.71550246505628	27.900196144263223	27.53838949656306
46-47	21.227569511322372	24.937048850092328	26.454944647164325	27.380436991420975
48-49	21.184250632810738	26.079754032097753	26.339504618524458	26.39649071656705
50-51	23.889841140817445	25.44618402219434	24.693414147125868	25.970560689862342
52-53	18.70482619682966	27.24433173697139	26.111120928835245	27.939721137363705
54-55	19.506699712396124	25.63252972127606	25.848563968668408	29.012206597659407
56-57	20.369871304931763	26.262099677930983	25.127126693734013	28.24090232340324
58-59	19.508153986859952	26.417503567783466	25.905421705378405	28.168920739978176
60-61	18.608740991458735	25.86815604936526	25.71924723723339	29.803855721942618
62-63	19.904570456603857	26.07656453643774	25.04716251739601	28.971702489562396
64-65	20.50043742985658	25.5419269889803	24.470886612878996	29.486748968284125
66-67	19.315927377485963	28.563412144697132	23.094631077098455	29.02602940071845
68-69	20.16910293279616	29.642931294202878	21.87515185248994	28.312813920511022
70-71	20.832615485199078	29.5965472609124	21.607007964801145	27.963829289087382
72-73	21.7162547213571	27.701720672365425	21.87440637907804	28.707618227199433
74-75	21.34626939525678	27.482946311808576	22.953999222422507	28.21678507051214
76	20.74446466752664	28.671520206393243	22.039723630966055	28.544291495114066
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	3.5
15	8.0
16	13.0
17	14.0
18	33.0
19	58.5
20	69.0
21	73.0
22	108.0
23	160.5
24	237.5
25	297.0
26	309.0
27	437.5
28	672.0
29	790.0
30	876.5
31	1061.0
32	1360.0
33	1561.0
34	1668.0
35	2062.0
36	2631.0
37	2913.0
38	3235.5
39	3867.5
40	4458.0
41	4936.5
42	5134.0
43	5397.5
44	5856.0
45	6176.5
46	6302.0
47	6361.5
48	6341.0
49	6117.0
50	5973.0
51	5817.0
52	5392.0
53	4919.5
54	4716.0
55	4397.5
56	3791.0
57	3322.0
58	3141.0
59	2902.0
60	2528.0
61	2206.0
62	2019.0
63	1865.0
64	1582.5
65	1388.5
66	1209.0
67	1095.0
68	1007.0
69	905.5
70	835.5
71	779.0
72	702.0
73	602.0
74	570.0
75	561.0
76	536.0
77	435.5
78	341.0
79	322.0
80	303.5
81	284.0
82	225.5
83	168.0
84	149.0
85	114.5
86	88.0
87	77.0
88	71.5
89	57.0
90	41.5
91	28.0
92	21.0
93	14.5
94	6.5
95	10.0
96	15.0
97	9.5
98	4.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.835014931175235E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.001767002986235047
7	0.008835014931175235
8	0.004417507465587617
9	0.012369020903645327
10-11	0.0030922552259113317
12-13	0.0
14-15	8.835014931175235E-4
16-17	4.4175074655876173E-4
18-19	0.001325252239676285
20-21	0.0
22-23	0.001767002986235047
24-25	0.00265050447935257
26-27	8.835014931175235E-4
28-29	0.007068011944940188
30-31	4.4175074655876173E-4
32-33	4.4175074655876173E-4
34-35	0.003534005972470094
36-37	0.01811178060890923
38-39	0.011485519410527802
40-41	8.835014931175235E-4
42-43	8.835014931175235E-4
44-45	0.003534005972470094
46-47	0.00265050447935257
48-49	4.4175074655876173E-4
50-51	0.003534005972470094
52-53	0.01060201791741028
54-55	0.00839326418461647
56-57	0.01016026717085152
58-59	0.01811178060890923
60-61	0.026063294046966938
62-63	0.011927270157086566
64-65	0.022087537327938084
66-67	0.022529288074496844
68-69	4.4175074655876173E-4
70-71	4.4175074655876173E-4
72-73	0.0030922552259113317
74-75	0.012369020903645327
76	0.003534005972470094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	113186.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.66016998568728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.06613651901695	60.450055660594074
2	9.106065512815901	13.415086671496477
3	4.617801926283089	10.204442245507394
4	2.1637700454583615	6.375346774336049
5	0.9703381190553296	3.573763539660382
6	0.5181533590011155	2.2900358701606205
7	0.19910522591246568	1.0266287350025622
8	0.12713948160675517	0.7492092661636598
9	0.08635889316685258	0.5725089675401551
>10	0.14513091768318281	1.3429222695386356
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0035340059724700934	0.0
6	0.0	0.0	0.0	0.0035340059724700934	0.0
7	0.0	0.0	0.0	0.0035340059724700934	0.0
8	0.0	0.0	0.0	0.004417507465587617	0.0
9	0.0	0.0	0.0	0.004417507465587617	0.0
10	0.0	0.0	0.0	0.004417507465587617	0.0
11	0.0	0.0	0.0	0.004417507465587617	0.0
12	0.0	0.0	0.0	0.004417507465587617	0.0
13	0.0	0.0	0.0	0.00530100895870514	0.0
14	0.0	0.0	0.0	0.007068011944940187	0.0
15	0.0	0.0	0.0	0.007068011944940187	0.0
16	0.0	0.0	0.0	0.008835014931175235	0.0
17	0.0	0.0	0.0	0.009718516424292757	0.0
18	0.0	0.0	0.0	0.013252522396762851	0.0
19	0.0	0.0	0.0	0.015019525382997898	0.0
20	0.0	0.0	0.0	0.015019525382997898	0.0
21	0.0	0.0	0.0	0.01767002986235047	0.0
22	0.0	0.0	0.0	0.03799056420405351	0.0
23	0.0	0.0	0.0	0.03887406569717103	0.0
24	0.0	0.0	0.0	0.04064106868340608	0.0
25	0.0	0.0	0.0	0.04240807166964112	0.0
26	0.0	0.0	0.0	0.04329157316275865	0.0
27	0.0	0.0	0.0	0.06361210750446168	0.0
28	0.0	0.0	0.0	0.09718516424292757	0.0
29	0.0	0.0	0.0	0.13340872546074603	0.0
30	0.0	0.0	0.0	0.18376831056844486	0.0
31	0.0	0.0	0.0	0.23589489866237873	0.0
32	0.0	0.0	0.0	0.28625448377007756	0.0
33	0.0	0.0	0.0	0.32954605693283623	0.0
34	0.0	0.0	0.0	0.3931581644372979	0.0
35	0.0	0.0	0.0	0.5053628540632233	0.0
36	0.0	0.0	0.0	0.6785291467142579	0.0
37	0.0	0.0	0.0	0.9974731857296839	0.0
38	0.0	0.0	0.0	1.3632428038803386	0.0
39	0.0	0.0	0.0	1.7837895146042797	0.0
40	0.0	0.0	0.0	2.275016344777623	0.0
41	0.0	0.0	0.0	2.8616613362076584	0.0
42	0.0	0.0	0.0	3.616171611330023	0.0
43	0.0	0.0	0.0	4.445779513367378	0.0
44	0.0	0.0	0.0	5.151697206368278	0.0
45	0.0	0.0	0.0	5.758662732140017	0.0
46	0.0	0.0	0.0	6.510522502783029	0.0
47	0.0	0.0	0.0	7.231459721166929	0.0
48	0.0	0.0	0.0	7.8896683335394835	0.0
49	0.0	0.0	0.0	8.466594808545226	0.0
50	0.0	0.0	0.0	9.18223101797042	0.0
51	0.0	0.0	0.0	9.893449719930027	0.0
52	0.0	0.0	0.0	10.65591150849045	0.0
53	0.0	0.0	0.0	11.463431873199866	0.0
54	0.0	0.0	0.0	12.223243157280935	0.0
55	0.0	0.0	0.0	12.920325835350662	0.0
56	0.0	0.0	0.0	13.604155991023624	0.0
57	0.0	0.0	0.0	14.26589860936865	0.0
58	0.0	0.0	0.0	14.831339564963864	0.0
59	0.0	0.0	0.0	15.353488947396322	0.0
60	0.0	0.0	0.0	15.78905518350326	0.0
61	0.0	0.0	0.0	16.30325305249766	0.0
62	0.0	0.0	0.0	16.8298199423957	0.0
63	0.0	0.0	0.0	17.381124874101037	0.0
64	0.0	0.0	0.0	17.943031823723782	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCGC	65	0.0	64.61539	49
ACGATAT	70	0.0	55.000004	24
CGGTGGT	85	0.0	53.52941	46
GTGGTCG	105	0.0	53.333332	48
AGATCCC	20	0.006935931	52.5	40
GCGAACT	20	0.006935931	52.5	28
TCCGAAA	20	0.006935931	52.5	20
GATCCCG	20	0.006935931	52.5	41
CGAGACG	20	0.006935931	52.5	69
GCCGTCT	20	0.006935931	52.5	55
CGATATG	70	7.2759576E-12	50.000004	25
ATCTCGG	105	0.0	49.999996	42
ATATGCA	80	0.0	48.125	27
GGTGGTC	125	0.0	47.600002	47
AGATCTC	140	0.0	47.500004	40
TCTCGGG	30	5.856837E-4	46.666664	45
TCTCGGC	60	4.8730726E-9	46.666664	43
GACGCTT	30	5.856837E-4	46.666664	14
TGTAGAT	160	0.0	45.9375	37
TCGGTGG	85	1.8189894E-12	45.294117	45
>>END_MODULE