FastQCFastQC Report
Wed 25 May 2016
SRR1779868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113186
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1612914.249995582492533No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG22221.963140317707137No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC10890.9621331260049829No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT4960.43821674058629156Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3690.3260120509603661No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT3110.2747689643595498No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT2880.2544484300178467No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2030.17935080310285723No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT1320.11662219709151309No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT1270.11220468962592547No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1240.10955418514657289No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACT150.002216541270.025256
CGTCTTA207.883734E-570.0251948
CTAGACT150.002220432569.9942554
GGGTACT150.002220432569.99425526
CGGCTGA150.002220432569.9942556
GTCTTAT308.313791E-658.35433249
CGTATTA252.38277E-455.99540715
ATATACT252.38277E-455.99540719
TCTCGTA25750.052.59762638
CGGCAAG200.006926059352.51889452
CTATAAC200.00693818352.495692
GGATAGT200.00693818352.4956922
CGCCTAG200.00693818352.4956931
TACTGCG200.00693818352.4956947
TAGATCT200.00693818352.4956934
AGTTAAT200.00693818352.4956911
ATGCCGC200.00693818352.4956944
CCGCCTA200.00693818352.4956930
GATCTCG25650.052.3933636
ATCTCGT25900.052.29300337
Produced by FastQC (version 0.11.5)