##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779868_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 113186 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.52982701040765 34.0 34.0 34.0 34.0 34.0 2 33.640034986659124 34.0 34.0 34.0 34.0 34.0 3 33.749977912462676 34.0 34.0 34.0 34.0 34.0 4 33.52874913858604 34.0 34.0 34.0 34.0 34.0 5 33.61254042019331 34.0 34.0 34.0 34.0 34.0 6 37.256462813422154 38.0 38.0 38.0 36.0 38.0 7 37.30342975279628 38.0 38.0 38.0 37.0 38.0 8 37.18898980439277 38.0 38.0 38.0 36.0 38.0 9 37.12960966904034 38.0 38.0 38.0 36.0 38.0 10-11 37.06065237750252 38.0 37.5 38.0 35.5 38.0 12-13 37.231141660629405 38.0 38.0 38.0 36.0 38.0 14-15 37.259082395349246 38.0 38.0 38.0 36.0 38.0 16-17 37.14608697188699 38.0 38.0 38.0 36.0 38.0 18-19 36.99855989256622 38.0 38.0 38.0 35.5 38.0 20-21 37.0397178096231 38.0 38.0 38.0 35.5 38.0 22-23 37.03247309737954 38.0 37.5 38.0 35.5 38.0 24-25 37.187483434347 38.0 38.0 38.0 36.0 38.0 26-27 37.08985651935752 38.0 38.0 38.0 36.0 38.0 28-29 36.62989238951814 38.0 37.0 38.0 34.0 38.0 30-31 36.879914477055465 38.0 37.0 38.0 35.0 38.0 32-33 36.91613803827328 38.0 37.5 38.0 36.0 38.0 34-35 36.91555492728783 38.0 38.0 38.0 36.0 38.0 36-37 36.9132092308236 38.0 38.0 38.0 35.5 38.0 38-39 35.927093456787944 38.0 37.0 38.0 32.5 38.0 40-41 36.586724506564416 38.0 37.0 38.0 34.5 38.0 42-43 37.0769706500804 38.0 37.5 38.0 36.0 38.0 44-45 36.84183114519463 38.0 37.5 38.0 35.0 38.0 46-47 36.993007085681974 38.0 38.0 38.0 36.0 38.0 48-49 36.612558090223175 38.0 37.0 38.0 34.0 38.0 50-51 36.7546339653314 38.0 37.0 38.0 34.5 38.0 52-53 36.6821824253883 38.0 37.0 38.0 34.0 38.0 54-55 36.682178007880836 38.0 37.0 38.0 34.5 38.0 56-57 36.55000618451045 38.0 37.0 38.0 34.0 38.0 58-59 36.632445708833245 38.0 37.0 38.0 34.0 38.0 60-61 36.27134539607372 38.0 37.0 38.0 34.0 38.0 62-63 36.10146131146962 38.0 37.0 38.0 34.0 38.0 64-65 36.51816037319103 38.0 37.0 38.0 34.0 38.0 66-67 36.232944003675364 38.0 37.0 38.0 33.0 38.0 68-69 36.26162687964943 38.0 37.0 38.0 34.0 38.0 70-71 35.05895163712827 38.0 36.0 38.0 28.0 38.0 72-73 32.98258618557065 38.0 33.0 38.0 16.0 38.0 74-75 32.69896453625007 38.0 33.0 38.0 11.0 38.0 76 32.505239163854185 38.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 2.0 23 7.0 24 28.0 25 65.0 26 151.0 27 286.0 28 482.0 29 755.0 30 1234.0 31 1936.0 32 2911.0 33 4531.0 34 8120.0 35 15422.0 36 18954.0 37 58301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.060853576606206 15.354831865917046 11.20451893497401 39.37979562250274 2 17.115190924672664 15.546092272895942 43.619352216705245 23.71936458572615 3 16.200766879296026 17.7725160355521 41.839096708073434 24.187620377078435 4 11.987348258618557 28.929372890640188 32.23455197639284 26.848726874348415 5 11.467849380665452 30.47108299613026 44.692806530843036 13.368261092361248 6 25.199229586698003 45.37310268054353 16.416341243616703 13.011326489141767 7 23.22018624211475 26.216139805276274 18.103829095471173 32.45984485713781 8 21.153676249712863 44.37562949481384 15.955153464209355 18.515540791263938 9 19.778947926421996 16.2449419539519 16.115067234463627 47.861042885162476 10-11 20.84489247786829 29.677698655310724 28.597176329227995 20.88023253759299 12-13 20.75168307034439 18.552206103228315 30.441485696110824 30.25462513031647 14-15 19.072146731927976 18.89677168554415 20.032512854946724 41.99856872758115 16-17 28.802148675631262 29.276147226688813 19.125598572261588 22.79610552541834 18-19 28.987242238439382 22.936140512077465 20.268849504355664 27.80776774512749 20-21 19.646422702454366 20.699556482250454 20.25427172971922 39.399749085575955 22-23 19.084074002085064 30.19189652430513 20.34527238351033 30.378757090099484 24-25 28.545617592359445 29.78296689947034 20.148782308687952 21.522633199482264 26-27 28.706288763628013 31.13989362642023 20.768911349460183 19.38490626049158 28-29 19.469280653084304 22.341102256462815 29.54384817910342 28.64576891134946 30-31 27.121728835721733 20.528598943332213 32.625943137843905 19.72372908310215 32-33 20.738430547947626 21.330818299082928 29.52352764476172 28.40722350820773 34-35 18.973636315445372 38.26356616542682 22.957786298658846 19.805011220468963 36-37 28.28176629618504 31.685455798420296 20.75433357482374 19.278444330570917 38-39 19.755093386107824 22.84911561500539 29.764723552382804 27.63106744650398 40-41 18.63878924955383 20.00910006537911 31.58429487745834 29.767815807608716 42-43 27.780182886424882 19.63599416883863 30.97009321023104 21.613729734505455 44-45 18.975845069178167 28.995635502624 32.246920997296485 19.781598430901347 46-47 27.523280264343647 19.882759351863307 24.367854681674412 28.226105702118637 48-49 26.364568056120014 19.335430176877 22.668439559662858 31.63156220734013 50-51 19.304470001899872 19.235986232553206 32.160861746733765 29.298682018813153 52-53 16.940644112136315 19.602644324247887 41.43599533354544 22.02071623007035 54-55 16.78063269933742 19.48399245039494 33.943607809509494 29.791767040758145 56-57 26.014179506899694 19.544116477046703 24.744300350951637 29.69740366510197 58-59 17.177605034403822 19.940516963271655 40.10685734235449 22.77502065997004 60-61 25.88978744089443 28.604003712050908 24.61133943170268 20.894869415351984 62-63 16.716599941670864 40.460976924640526 21.047538245353554 21.774884888335055 64-65 16.500689240774776 41.39730312455818 20.2583769263396 21.843630708327442 66-67 16.47494000715937 41.361693853109244 19.97816835118019 22.1851977885512 68-69 16.29916633163831 41.55441375928323 19.59893792329544 22.547481985783016 70-71 16.928521565474032 39.27823482110509 20.00936668816881 23.783876925252063 72-73 17.83487616817268 34.915052029251264 20.79977023175662 26.450301570819434 74-75 18.0375454517339 33.23510309756604 21.466472857086053 27.260878593614002 76 18.056181463121526 33.94480820719455 20.858185545511578 27.140824784172345 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 7.0 16 13.0 17 15.0 18 21.5 19 44.5 20 64.5 21 68.0 22 127.5 23 206.0 24 255.0 25 285.0 26 351.0 27 486.0 28 621.5 29 688.0 30 821.0 31 1025.0 32 1288.5 33 1481.0 34 1603.0 35 2012.5 36 2505.0 37 2710.0 38 3011.5 39 5736.0 40 9802.5 41 10885.5 42 10325.0 43 9173.5 44 7137.0 45 5822.0 46 5392.0 47 5255.0 48 4830.5 49 4527.5 50 4512.0 51 4186.0 52 3680.0 53 3310.5 54 3121.0 55 3010.5 56 2725.0 57 2378.0 58 2206.0 59 2015.5 60 1787.0 61 1565.0 62 1381.0 63 1376.0 64 1248.0 65 1090.5 66 943.0 67 830.0 68 886.0 69 889.5 70 716.5 71 596.0 72 585.5 73 526.0 74 450.5 75 424.0 76 416.0 77 384.0 78 344.0 79 328.0 80 281.5 81 222.0 82 178.0 83 147.0 84 106.5 85 62.0 86 62.5 87 67.0 88 58.5 89 34.0 90 13.0 91 5.0 92 2.0 93 3.5 94 6.0 95 4.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054777092573286446 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.4175074655876173E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 8.835014931175235E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.01811178060890923 52-53 0.03357305673846588 54-55 0.05963635078543283 56-57 0.05742759705263902 58-59 0.039315816443729786 60-61 0.03622356121781846 62-63 0.030922552259113317 64-65 0.01590302687611542 66-67 0.04196632092308236 68-69 0.01016026717085152 70-71 0.01678652836923294 72-73 0.025179792553849417 74-75 0.014577774636439136 76 0.015019525382997898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 113186.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.11628646652413 #Duplication Level Percentage of deduplicated Percentage of total 1 44.12933863351386 14.613998197656954 2 20.708054317957476 13.715477179156432 3 14.614625296801217 14.51946353789338 4 9.076114505242376 12.022688318343258 5 5.007603446895926 8.291661512907957 6 2.72656937811808 5.417631155796653 7 1.3205986713977003 3.061332673652218 8 0.7896913267347865 2.092131535702295 9 0.4428674332364005 1.3199512307175798 >10 1.1231758397140037 5.240047355680031 >50 0.03201451324600486 0.719170215397664 >100 0.0213430088306699 1.811178060890923 >500 0.0 0.0 >1k 0.005335752207667475 2.9252734437121197 >5k 0.0 0.0 >10k+ 0.0026678761038337376 14.249995582492533 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 16129 14.249995582492533 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 2222 1.963140317707137 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 1089 0.9621331260049829 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 496 0.43821674058629156 Illumina PCR Primer Index 9 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 369 0.3260120509603661 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 311 0.2747689643595498 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT 288 0.2544484300178467 No Hit CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC 203 0.17935080310285723 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT 132 0.11662219709151309 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT 127 0.11220468962592547 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC 124 0.10955418514657289 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.835014931175233E-4 0.0 3 0.0 0.0 0.0 8.835014931175233E-4 0.0 4 0.0 0.0 0.0 8.835014931175233E-4 0.0 5 0.0 0.0 0.0 0.00530100895870514 0.0 6 0.0 0.0 0.0 0.00530100895870514 0.0 7 0.0 0.0 0.0 0.00530100895870514 0.0 8 0.0 0.0 0.0 0.006184510451822663 0.0 9 0.0 0.0 0.0 0.006184510451822663 0.0 10 0.0 0.0 0.0 0.006184510451822663 0.0 11 0.0 0.0 0.0 0.006184510451822663 0.0 12 0.0 0.0 0.0 0.007068011944940187 0.0 13 0.0 0.0 0.0 0.008835014931175235 0.0 14 0.0 0.0 0.0 0.009718516424292757 0.0 15 0.0 0.0 0.0 0.009718516424292757 0.0 16 0.0 0.0 0.0 0.01060201791741028 0.0 17 0.0 0.0 0.0 0.011485519410527804 0.0 18 0.0 0.0 0.0 0.016786528369232945 0.0 19 0.0 0.0 0.0 0.02032053434170304 0.0 20 0.0 0.0 0.0 0.02032053434170304 0.0 21 0.0 0.0 0.0 0.02120403583482056 0.0 22 0.0 0.0 0.0 0.025621543300408178 0.0 23 0.0 0.0 0.0 0.028272047779760747 0.0 24 0.0 0.0 0.0 0.03180605375223084 0.0 25 0.0 0.0 0.0 0.03357305673846589 0.0 26 0.0 0.0 0.0 0.03445655823158341 0.0 27 0.0 0.0 0.0 0.056544095559521494 0.0 28 0.0 0.0 0.0 0.09188415528422243 0.0 29 0.0 0.0 0.0 0.13075822098139345 0.0 30 0.0 0.0 0.0 0.18288480907532734 0.0 31 0.0 0.0 0.0 0.2385454031417313 0.0 32 0.0 0.0 0.0 0.2889049882494301 0.0 33 0.0 0.0 0.0 0.33484706589154134 0.0 34 0.0 0.0 0.0 0.4028766808615907 0.0 35 0.0 0.0 0.0 0.5274503913911615 0.0 36 0.0 0.0 0.0 0.7297722333150743 0.0 37 0.0 0.0 0.0 1.0778718216033785 0.0 38 0.0 0.0 0.0 1.4604279681232661 0.0 39 0.0 0.0 0.0 1.8862756878059124 0.0 40 0.0 0.0 0.0 2.395172547841606 0.0 41 0.0 0.0 0.0 3.001254572120227 0.0 42 0.0 0.0 0.0 3.7911049069672926 0.0 43 0.0 0.0 0.0 4.6595868747018185 0.0 44 0.0 0.0 0.0 5.3822910960719526 0.0 45 0.0 0.0 0.0 6.012227660664746 0.0 46 0.0 0.0 0.0 6.800310992525578 0.0 47 0.0 0.0 0.0 7.551287261675472 0.0 48 0.0 0.0 0.0 8.244835933772729 0.0 49 0.0 0.0 0.0 8.86505398194123 0.0 50 0.0 0.0 0.0 9.60012722421501 0.0 51 0.0 0.0 0.0 10.330782959023201 0.0 52 0.0 0.0 0.0 11.093244747583624 0.0 53 0.0 0.0 0.0 11.895464103334335 0.0 54 0.0 0.0 0.0 12.657925891894758 0.0 55 0.0 0.0 0.0 13.356775572950719 0.0 56 0.0 0.0 0.0 14.040605728623682 0.0 57 0.0 0.0 0.0 14.717367872351705 0.0 58 0.0 0.0 0.0 15.290760341384978 0.0 59 0.0 0.0 0.0 15.832346756666018 0.0 60 0.0 0.0 0.0 16.268796494266077 0.0 61 0.0 0.0 0.0 16.78476136624671 0.0 62 0.0 0.0 0.0 17.320163271075927 0.0 63 0.0 0.0 0.0 17.877652713233086 0.0 64 0.0 0.0 0.0 18.447511176293887 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGTACT 15 0.0022165412 70.0252 56 CGTCTTA 20 7.883734E-5 70.02519 48 CTAGACT 15 0.0022204325 69.994255 4 GGGTACT 15 0.0022204325 69.994255 26 CGGCTGA 15 0.0022204325 69.994255 6 GTCTTAT 30 8.313791E-6 58.354332 49 CGTATTA 25 2.38277E-4 55.995407 15 ATATACT 25 2.38277E-4 55.995407 19 TCTCGTA 2575 0.0 52.597626 38 CGGCAAG 20 0.0069260593 52.518894 52 CTATAAC 20 0.006938183 52.49569 2 GGATAGT 20 0.006938183 52.49569 22 CGCCTAG 20 0.006938183 52.49569 31 TACTGCG 20 0.006938183 52.49569 47 TAGATCT 20 0.006938183 52.49569 34 AGTTAAT 20 0.006938183 52.49569 11 ATGCCGC 20 0.006938183 52.49569 44 CCGCCTA 20 0.006938183 52.49569 30 GATCTCG 2565 0.0 52.39336 36 ATCTCGT 2590 0.0 52.293003 37 >>END_MODULE