##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779867_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47037
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.797691179284392	23.0	12.0	33.0	12.0	34.0
2	23.372068796904564	23.0	12.0	34.0	12.0	34.0
3	22.88521801985671	23.0	11.0	34.0	11.0	34.0
4	23.167081233922232	23.0	12.0	34.0	12.0	34.0
5	22.576439823968364	23.0	11.0	34.0	11.0	34.0
6	25.414057018942536	27.0	12.0	37.0	11.0	38.0
7	25.589089440227905	27.0	12.0	37.0	11.0	38.0
8	25.116185130854433	26.0	11.0	37.0	11.0	38.0
9	25.84803452601144	28.0	12.0	37.0	11.0	38.0
10-11	24.829676637540658	26.0	11.5	37.0	11.0	38.0
12-13	25.05481854710122	26.0	11.0	37.0	11.0	38.0
14-15	24.25774390373536	26.0	11.0	36.0	11.0	38.0
16-17	24.441971214150563	25.5	11.0	36.5	11.0	38.0
18-19	24.40727512383868	26.0	11.0	37.0	11.0	38.0
20-21	24.407647171375725	24.5	11.0	36.0	11.0	38.0
22-23	24.604449688543063	25.0	11.0	37.0	10.5	38.0
24-25	24.75966792099836	26.0	11.0	37.0	10.0	38.0
26-27	23.97676297382911	23.5	11.0	37.0	10.0	38.0
28-29	24.229478920849544	25.0	11.0	37.0	11.0	38.0
30-31	24.32806088823692	25.5	11.0	37.0	11.0	38.0
32-33	24.562961073197695	26.0	11.0	37.0	11.0	38.0
34-35	24.662978081085104	26.0	11.0	37.0	11.0	38.0
36-37	24.419403873546358	26.0	11.0	37.0	11.0	38.0
38-39	24.324266003359057	26.0	11.0	37.0	11.0	38.0
40-41	24.27637817037651	26.0	11.0	37.0	11.0	38.0
42-43	24.536003571656355	26.0	11.0	37.0	11.0	38.0
44-45	24.693656057996897	26.0	11.0	37.0	11.0	38.0
46-47	24.560845717201353	25.5	11.0	37.0	11.0	38.0
48-49	24.19228479707464	25.5	11.0	37.0	11.0	38.0
50-51	23.88657865084933	23.5	11.0	36.5	11.0	38.0
52-53	24.333227034037034	25.0	11.0	37.0	11.0	38.0
54-55	24.34144396964092	25.0	11.0	37.0	11.0	38.0
56-57	24.228054510279144	24.5	11.0	37.0	11.0	38.0
58-59	24.178912345600274	24.5	11.0	37.0	11.0	38.0
60-61	24.38198652124923	25.0	11.0	37.0	11.0	38.0
62-63	24.149573739821843	24.0	11.0	37.0	11.0	38.0
64-65	24.09499968110211	24.5	11.0	37.0	11.0	38.0
66-67	24.430395220783637	25.0	11.0	37.0	11.0	38.0
68-69	24.1330441992474	24.5	11.0	37.0	11.0	38.0
70-71	24.253002955120436	24.5	11.0	37.0	11.0	38.0
72-73	23.81845143185152	23.5	11.0	37.0	11.0	38.0
74-75	23.731849395157006	23.5	11.0	37.0	11.0	38.0
76	23.472053915003084	24.0	11.0	36.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	47.0
13	517.0
14	1806.0
15	3069.0
16	3937.0
17	4522.0
18	4428.0
19	3816.0
20	2865.0
21	2083.0
22	1339.0
23	950.0
24	708.0
25	545.0
26	483.0
27	422.0
28	398.0
29	434.0
30	476.0
31	520.0
32	689.0
33	865.0
34	1119.0
35	1805.0
36	3101.0
37	6090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.402853073112656	21.3236388375109	9.464889342432553	45.8086187469439
2	12.058592172119821	21.425686161957607	28.24797499840551	38.26774666751706
3	20.515764185641093	20.11820481748411	32.79333290813614	26.57269808873865
4	20.07781108489062	30.809788039203177	19.92686608414652	29.185534791759675
5	11.010481110615048	27.440100346535708	26.496162595403618	35.05325594744563
6	17.548633995960454	46.56957584777294	11.816732220686722	24.065057935579887
7	16.067783636672903	38.08258207178091	12.80192209559449	33.047712195951696
8	14.599455736031974	42.669444680670125	17.059273747767666	25.671825835530232
9	19.86094573907127	21.574672563361116	14.777172988603503	43.78720870896411
10-11	15.634832893953568	33.17671570711796	21.079598605323582	30.1088527936049
12-13	15.429506872322557	24.107873672573426	25.25804428475758	35.20457517034643
14-15	26.43238301762442	27.875927461360206	18.270723047813423	27.420966473201947
16-17	28.339392393222358	24.660373748325785	16.97068265408083	30.029551204371025
18-19	19.875624534920806	24.430743063675987	21.313915169554587	34.37971723184862
20-21	26.070965410208984	23.474073601632757	18.60769181708017	31.847269171078086
22-23	28.57142857142857	23.06081447386604	17.109053607304965	31.258703347400424
24-25	19.889444031040714	31.573296481343682	16.209205910492187	32.32805357712342
26-27	24.098050470905882	32.0758126581202	18.195250547441375	25.630886323532536
28-29	20.79156346008696	29.668215208308972	21.734508382323238	27.80571294928083
30-31	22.875851732165444	26.756880295089985	22.35816865625631	28.00909931648826
32-33	18.0985181878096	30.19963007844888	23.571868954227522	28.129982779514002
34-35	16.73594352961187	35.68732924404945	20.198369247451282	27.37835797888739
36-37	23.672319387592367	26.310137685396846	21.22587847536016	28.79166445165063
38-39	24.788977951650967	22.7021453022346	25.597984393936173	26.910892352178255
40-41	19.64963379503152	28.86800676070711	22.508052257289553	28.97430718697182
42-43	14.13370467615575	28.16323493457209	25.074144547319634	32.62891584195253
44-45	13.936282169850431	23.978696941671714	29.818966525284097	32.26605436319376
46-47	17.598783871413538	27.251273001732773	25.5248806726977	29.625062454155994
48-49	18.228397095872353	29.232617222795064	23.094830610270748	29.444155071061832
50-51	25.423890973647005	27.981587983288865	18.280198577639815	28.31432246542432
52-53	13.035158035742764	31.659242406523425	22.517302601503282	32.78829695623053
54-55	16.253282444371205	26.43497304940411	22.35676848002892	34.954976026195766
56-57	19.17479082722914	28.95780397826942	19.804169634598825	32.063235559902616
58-59	17.69665011747446	29.20064212283257	20.324676015011217	32.778031744681755
60-61	14.396895433522939	26.790707564722773	21.83828610919143	36.97411089256286
62-63	17.55387575883222	27.793193633783055	20.407403863532465	34.24552674385226
64-65	17.76832704268779	26.681197171867527	19.87666790707565	35.67380787836904
66-67	15.071973804511918	35.22144968212456	16.231847079585805	33.474729433777725
68-69	16.806276043881283	37.48618079768688	15.010842758737988	30.69670039969385
70-71	16.90283078035143	38.287287532023	14.668395820267238	30.14148586735833
72-73	19.73230991665249	32.97329477802347	15.66592957986052	31.62846572546351
74-75	17.26784670174879	31.626003295593474	20.34550576728858	30.76064423536916
76	15.60818999425935	36.572193991452814	16.86900686751855	30.950609146769292
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	3.0
14	4.0
15	3.5
16	4.0
17	3.0
18	3.0
19	11.5
20	17.5
21	15.0
22	26.0
23	44.0
24	55.5
25	60.0
26	82.0
27	107.0
28	152.0
29	194.0
30	208.5
31	249.0
32	337.5
33	400.0
34	457.0
35	599.5
36	793.5
37	902.0
38	966.0
39	1167.0
40	1405.0
41	1599.5
42	1693.0
43	1920.0
44	2317.0
45	2489.5
46	2492.0
47	2564.0
48	2722.0
49	2799.5
50	2791.0
51	2785.5
52	2723.0
53	2619.0
54	2572.0
55	2438.5
56	2159.5
57	1910.0
58	1806.0
59	1676.0
60	1432.5
61	1199.5
62	1080.0
63	1009.0
64	845.0
65	651.5
66	500.5
67	450.0
68	413.5
69	320.5
70	255.0
71	246.0
72	232.0
73	206.0
74	148.0
75	102.0
76	80.5
77	60.0
78	57.0
79	53.0
80	43.5
81	29.0
82	25.5
83	27.0
84	26.0
85	19.0
86	12.0
87	11.0
88	9.0
89	6.0
90	4.0
91	3.5
92	4.0
93	3.5
94	5.0
95	5.0
96	3.0
97	5.0
98	5.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.00425197185194634
7	0.01062992962986585
8	0.00212598592597317
9	0.01062992962986585
10-11	0.00212598592597317
12-13	0.001062992962986585
14-15	0.0
16-17	0.0
18-19	0.00425197185194634
20-21	0.0
22-23	0.001062992962986585
24-25	0.00425197185194634
26-27	0.0
28-29	0.007440950740906095
30-31	0.001062992962986585
32-33	0.0
34-35	0.007440950740906095
36-37	0.020196866296745115
38-39	0.00850394370389268
40-41	0.001062992962986585
42-43	0.001062992962986585
44-45	0.0031889788889597555
46-47	0.005314964814932925
48-49	0.001062992962986585
50-51	0.005314964814932925
52-53	0.013818908518825604
54-55	0.013818908518825604
56-57	0.013818908518825604
58-59	0.011692922592852435
60-61	0.020196866296745115
62-63	0.015944894444798775
64-65	0.020196866296745115
66-67	0.012755915555839022
68-69	0.00212598592597317
70-71	0.001062992962986585
72-73	0.01062992962986585
74-75	0.009566936666879265
76	0.00850394370389268
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	47037.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.87046367753045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.53140998038371	83.121797733699
2	3.2008037892923786	5.689138337904203
3	1.7319745466724081	4.617641431213725
4	0.8803406535572461	3.1294512830325063
5	0.37318788574709344	1.6582690222590728
6	0.14353380221042056	0.7653549333503412
7	0.08133582125257165	0.5059846503816144
8	0.033491220515764794	0.23811042370899504
9	0.011961150184201713	0.09566936666879265
>10	0.011961150184201713	0.17858281778174628
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.00212598592597317	0.0
5	0.0	0.0	0.0	0.01913387333375853	0.0
6	0.0	0.0	0.0	0.01913387333375853	0.0
7	0.0	0.0	0.0	0.01913387333375853	0.0
8	0.0	0.0	0.0	0.01913387333375853	0.0
9	0.0	0.0	0.0	0.01913387333375853	0.0
10	0.0	0.0	0.0	0.0212598592597317	0.0
11	0.0	0.0	0.0	0.0212598592597317	0.0
12	0.0	0.0	0.0	0.0212598592597317	0.0
13	0.0	0.0	0.0	0.0212598592597317	0.0
14	0.0	0.0	0.0	0.0212598592597317	0.0
15	0.0	0.0	0.0	0.0212598592597317	0.0
16	0.0	0.0	0.0	0.0212598592597317	0.0
17	0.0	0.0	0.0	0.0212598592597317	0.0
18	0.0	0.0	0.0	0.0212598592597317	0.0
19	0.0	0.0	0.0	0.03401577481557072	0.0
20	0.0	0.0	0.0	0.03401577481557072	0.0
21	0.0	0.0	0.0	0.03614176074154389	0.0
22	0.0	0.0	0.0	0.06803154963114144	0.0
23	0.0	0.0	0.0	0.07653549333503412	0.0
24	0.0	0.0	0.0	0.09779535259476582	0.0
25	0.0	0.0	0.0	0.10204732444671216	0.0
26	0.0	0.0	0.0	0.10204732444671216	0.0
27	0.0	0.0	0.0	0.12118119778047069	0.0
28	0.0	0.0	0.0	0.14669302889214875	0.0
29	0.0	0.0	0.0	0.17433084592979994	0.0
30	0.0	0.0	0.0	0.19984267704147798	0.0
31	0.0	0.0	0.0	0.22535450815315602	0.0
32	0.0	0.0	0.0	0.24448838148691457	0.0
33	0.0	0.0	0.0	0.2848821140804048	0.0
34	0.0	0.0	0.0	0.32527584667389503	0.0
35	0.0	0.0	0.0	0.40181134000892915	0.0
36	0.0	0.0	0.0	0.5187405659374535	0.0
37	0.0	0.0	0.0	0.7292131726087974	0.0
38	0.0	0.0	0.0	1.0225992303930949	0.0
39	0.0	0.0	0.0	1.2777175415098752	0.0
40	0.0	0.0	0.0	1.5774815570720921	0.0
41	0.0	0.0	0.0	1.9686629674511555	0.0
42	0.0	0.0	0.0	2.272678954865319	0.0
43	0.0	0.0	0.0	2.6319705763547847	0.0
44	0.0	0.0	0.0	2.9678763526585454	0.0
45	0.0	0.0	0.0	3.363309734889555	0.0
46	0.0	0.0	0.0	3.762995088972511	0.0
47	0.0	0.0	0.0	4.090396921572379	0.0
48	0.0	0.0	0.0	4.417798754172248	0.0
49	0.0	0.0	0.0	4.651657206029296	0.0
50	0.0	0.0	0.0	4.976933052703191	0.0
51	0.0	0.0	0.0	5.287326997895274	0.0
52	0.0	0.0	0.0	5.650870591236686	0.0
53	0.0	0.0	0.0	6.020792142356018	0.0
54	0.0	0.0	0.0	6.3524459468078325	0.0
55	0.0	0.0	0.0	6.667091863851861	0.0
56	0.0	0.0	0.0	7.024257499415354	0.0
57	0.0	0.0	0.0	7.272997852754215	0.0
58	0.0	0.0	0.0	7.506856304611263	0.0
59	0.0	0.0	0.0	7.683313136467037	0.0
60	0.0	0.0	0.0	7.919297574250058	0.0
61	0.0	0.0	0.0	8.089376448327911	0.0
62	0.0	0.0	0.0	8.221187575738249	0.0
63	0.0	0.0	0.0	8.391266449816102	0.0
64	0.0	0.0	0.0	8.5974870846355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGACG	15	0.002211615	70.00001	11
ACGATAC	25	2.7987971E-6	70.00001	24
CCCGTAT	15	0.002211615	70.00001	54
CGAGCGC	15	0.002211615	70.00001	25
GTGAGTT	15	0.002211615	70.00001	58
GACGATC	15	0.002211615	70.00001	23
GTAGTCT	15	0.002211615	70.00001	18
AAGCTGC	15	0.002211615	70.00001	15
TCGGCGG	20	7.849891E-5	70.0	45
CCGTATC	75	0.0	70.0	55
CTCGGCG	20	7.849891E-5	70.0	44
GTGTAGC	20	7.849891E-5	70.0	36
AGCTGCC	20	7.849891E-5	70.0	16
GTAAATC	20	7.849891E-5	70.0	38
ATCTCGG	125	0.0	67.2	42
CTCGGTG	90	0.0	66.11111	44
GTGGTCG	70	0.0	65.0	48
CGTATCA	65	0.0	64.61539	56
CGGTGGT	60	0.0	64.16667	46
GCCGTAT	50	1.2732926E-11	63.000004	54
>>END_MODULE