FastQCFastQC Report
Wed 25 May 2016
SRR1779867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47037
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2044043.45515232689159No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG24985.310712843080979No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC13712.9147267045092162No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT4901.0417331037268533RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT4630.9843314837255778No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4550.9673235963177923No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT4390.9333078215022217No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT1880.39968535408295597No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1770.3762995088972511No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT1760.3741735229712779No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1470.312519931118056No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1130.24023640963496823No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG1090.23173246593107552No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1090.23173246593107552No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC850.18070880370771944No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC750.15944894444798774No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG730.15519697259604143No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCG710.15094500074409506No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCG670.14244105704020238No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTCTGCCGT640.13606309926228288No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT590.12543316963241705RNA PCR Primer, Index 30 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT580.12330718370644386No Hit
CTTATACACATCTCCGAGCCCACGAGACATTACCGGATCTCGTATGCCGT520.11055126815060483No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCCGGATCTCGTATGCCGT490.10417331037268533No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT150.00221161570.0000132
CTATACA301.0030635E-770.000011
ACAGAGA150.00221161570.000018
CTTTAAC150.00221161570.0000163
CCAGTTG150.00221161570.0000132
GAGCTAA150.00221161570.000011
ACTCCAG150.00221161570.0000129
CATTCCC150.00221161570.0000128
GACATTA301.0030635E-770.0000126
ACATTAC252.7987971E-670.0000127
TTCCCGG150.00221161570.0000130
AACTCAC150.00221161570.000018
AATAGAC150.00221161570.0000145
TACCGGA150.00221161570.0000131
GCTAAAA150.00221161570.000013
TACGAAA150.00221161570.0000152
TATTTAC150.00221161570.000013
ATAGACT150.00221161570.0000146
AGTTGAC150.00221161570.0000134
CGAAAGT150.00221161570.0000154
Produced by FastQC (version 0.11.5)