##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779867_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47037
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.59908157407998	34.0	34.0	34.0	34.0	34.0
2	33.67991155898548	34.0	34.0	34.0	34.0	34.0
3	33.76663052490593	34.0	34.0	34.0	34.0	34.0
4	33.56532091757553	34.0	34.0	34.0	34.0	34.0
5	33.61674851712482	34.0	34.0	34.0	34.0	34.0
6	37.33951995237791	38.0	38.0	38.0	37.0	38.0
7	37.38752471458639	38.0	38.0	38.0	37.0	38.0
8	37.270000212598596	38.0	38.0	38.0	37.0	38.0
9	37.22333482152348	38.0	38.0	38.0	36.0	38.0
10-11	37.13907136934753	38.0	38.0	38.0	36.5	38.0
12-13	37.285105342602634	38.0	38.0	38.0	36.5	38.0
14-15	37.301156536343726	38.0	38.0	38.0	36.5	38.0
16-17	37.03519569700448	38.0	38.0	38.0	35.0	38.0
18-19	36.83757467525565	38.0	37.5	38.0	34.5	38.0
20-21	37.00741969088165	38.0	37.5	38.0	35.0	38.0
22-23	37.07325084507941	38.0	38.0	38.0	35.5	38.0
24-25	37.17933754278547	38.0	38.0	38.0	36.0	38.0
26-27	36.89575228011991	38.0	38.0	38.0	34.5	38.0
28-29	36.32756128154432	38.0	37.0	38.0	34.0	38.0
30-31	36.87514084656759	38.0	37.0	38.0	35.5	38.0
32-33	36.333110104811105	38.0	37.0	38.0	34.0	38.0
34-35	36.458925951910196	38.0	37.0	38.0	34.0	38.0
36-37	36.23906711737568	38.0	37.0	38.0	33.5	38.0
38-39	32.49942598379999	36.5	31.0	38.0	16.5	38.0
40-41	35.456077130769394	37.5	35.5	38.0	30.5	38.0
42-43	36.8342475072815	38.0	37.0	38.0	34.5	38.0
44-45	36.57219848204605	38.0	37.0	38.0	34.5	38.0
46-47	36.79030337819164	38.0	37.0	38.0	34.5	38.0
48-49	36.323936475540535	38.0	37.0	38.0	34.0	38.0
50-51	36.290228968684225	38.0	37.0	38.0	33.5	38.0
52-53	36.199980866126666	38.0	37.0	38.0	33.5	38.0
54-55	36.25225886004635	38.0	37.0	38.0	34.0	38.0
56-57	36.088855581776045	38.0	37.0	38.0	33.0	38.0
58-59	36.024406318430174	38.0	37.0	38.0	33.0	38.0
60-61	35.51220315921509	38.0	36.5	38.0	30.5	38.0
62-63	35.54017050407126	38.0	36.5	38.0	31.0	38.0
64-65	36.21963560601229	38.0	37.0	38.0	34.0	38.0
66-67	35.74484979909433	38.0	36.5	38.0	31.5	38.0
68-69	35.50598465038161	38.0	36.5	38.0	30.0	38.0
70-71	31.984916129855222	36.0	29.5	38.0	18.0	38.0
72-73	25.442449560983906	24.0	15.0	38.0	10.0	38.0
74-75	24.51059803984098	23.5	11.0	38.0	11.0	38.0
76	24.521249229330103	25.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	6.0
24	18.0
25	45.0
26	116.0
27	198.0
28	394.0
29	593.0
30	927.0
31	1427.0
32	2033.0
33	3283.0
34	5776.0
35	12668.0
36	8883.0
37	10668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.611425411544515	8.00757156833553	12.665362201709984	64.71564081840997
2	6.811658906818037	7.5515020090567	68.96273146671768	16.67410761740757
3	7.032761443119247	10.055913429853094	69.83438569636668	13.07693943066097
4	5.344728617896549	56.94240704126538	25.435295618343005	12.277568722495056
5	5.006696855666815	21.946552713821035	67.52556498075982	5.5211854497523225
6	10.510874418011353	68.13572294151413	13.723239152156813	7.630163488317708
7	9.347960116504028	20.29891362119183	12.900482598805196	57.45264366349895
8	8.09362842017986	68.24414822373875	8.172289899440866	15.489933456640518
9	8.250951378701874	14.307885281799434	8.204179688330465	69.23698365116823
10-11	9.073707932053491	40.27361438867275	35.18187809596701	15.470799583306757
12-13	9.268235644280034	10.821268363203435	40.91353615239067	38.99695984012586
14-15	8.90575504390161	9.086463847609329	14.605523311435679	67.4022577970534
16-17	37.30467504305122	35.01817717966707	8.819652613899697	18.85749516338202
18-19	39.76550375236516	16.042689797393543	8.997172438718454	35.194634011522844
20-21	11.297489210621425	10.017645683185577	9.029062227608053	69.65580287858495
22-23	9.518038990581882	39.08306227012777	8.674022577970533	42.72487616131981
24-25	37.678848566022495	38.26349469566511	8.775006909454259	15.282649828858133
26-27	39.532708293471096	41.821332142781216	9.8178030061441	8.82815655760359
28-29	11.312371112103238	40.72432340497906	10.878669983204711	37.084635499712995
30-31	8.685715500563386	15.602610710717096	63.638199715117885	12.073474073601632
32-33	38.00625039862236	9.463826349469565	18.20375449114527	34.326168760762805
34-35	36.94538342156175	9.96343304207326	9.941110189850544	43.150073346514446
36-37	43.14688436762549	34.90762591151647	10.181346599485511	11.764143121372536
38-39	11.76308012840955	16.11497331887663	36.92199757637604	35.19994897633777
40-41	8.65382571167379	9.525479941322788	41.43015073240215	40.39054361460127
42-43	37.3535727193486	9.172566277611242	38.701447796415586	14.772413206624572
44-45	11.056189808023472	37.1866828241597	41.75330059315007	10.003826774666752
46-47	37.12821821119544	11.237961604694178	16.98662754852563	34.64719263558475
48-49	35.62302017560644	8.838786487233454	11.388906605438272	44.14928673172184
50-51	13.47848736458256	8.98034254366847	39.75824199189887	37.782928099850096
52-53	7.571665532494046	8.823154134059203	67.91106668935012	15.694113644096634
54-55	7.321975497702909	8.966096647949634	45.311170665305426	38.400757189042025
56-57	36.07657537888859	9.10183461845254	17.702738633342197	37.118851369316666
58-59	10.302108699582089	10.141537021873903	64.2042131456098	15.352141132934207
60-61	35.7995810427145	35.84530480737535	18.439437278691663	9.915676871218485
62-63	9.616468361563898	70.89327676587239	9.448467255733835	10.041787616829883
64-65	6.896881678131346	74.06572610225714	9.049831485163253	9.98756073444826
66-67	7.464591042490749	73.76334481731955	8.578963038577687	10.19310110161201
68-69	7.2438500627219184	73.66529883273446	8.642867773690813	10.447983330852805
70-71	7.661230304705607	68.78947032681963	9.501584128941717	14.047715239533055
72-73	9.967462731003977	55.64829976819854	12.18765285073262	22.196584650064864
74-75	11.400748426603164	49.89368940295969	13.245237285252594	25.460324885184555
76	11.191205239431829	51.086586714297866	12.98588075189249	24.736327294377816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	3.0
14	3.0
15	1.0
16	1.0
17	0.0
18	2.0
19	12.0
20	22.0
21	24.0
22	16.5
23	22.0
24	43.0
25	51.0
26	65.5
27	90.0
28	116.0
29	132.0
30	148.0
31	195.0
32	282.5
33	339.0
34	363.0
35	427.0
36	498.0
37	529.0
38	580.5
39	680.5
40	917.0
41	3937.0
42	6769.0
43	8467.5
44	9390.0
45	6934.0
46	5254.0
47	4082.0
48	2248.5
49	1285.0
50	983.0
51	875.0
52	689.0
53	598.0
54	585.0
55	543.0
56	479.5
57	427.0
58	396.0
59	376.0
60	357.5
61	321.0
62	283.0
63	267.0
64	229.0
65	191.5
66	161.5
67	147.0
68	130.5
69	108.0
70	93.5
71	85.0
72	74.0
73	61.5
74	55.5
75	51.0
76	43.0
77	27.0
78	20.5
79	22.0
80	20.0
81	18.0
82	12.5
83	7.0
84	6.0
85	5.0
86	6.5
87	8.0
88	4.0
89	0.5
90	2.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04039373259349023
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.013818908518825604
52-53	0.027637817037651207
54-55	0.04464570444543657
56-57	0.052086655186342666
58-59	0.037204753704530476
60-61	0.032952781852584136
62-63	0.028700810000637796
64-65	0.018070880370771945
66-67	0.03188978888959755
68-69	0.00850394370389268
70-71	0.01700788740778536
72-73	0.02976380296362438
74-75	0.01062992962986585
76	0.01913387333375853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	47037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.953951144843423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.510953226761394	9.607330399472755
2	15.76080521018354	5.659374534940579
3	11.995263469508584	6.460871229032464
4	7.779751332149202	5.587091013457491
5	4.665482534043813	4.188192274167145
6	2.5458851391355832	2.7425218445053896
7	1.2551805802249854	1.5774815570720924
8	0.7341622261693309	1.0544890192826923
9	0.40260509177027826	0.6505516933477901
>10	1.0775606867969212	3.414333397112911
>50	0.10657193605683836	1.2840954992877947
>100	0.13025458851391356	6.093075663839105
>500	0.0	0.0
>1k	0.023682652457075192	8.225439547590195
>5k	0.0	0.0
>10k+	0.011841326228537596	43.45515232689159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	20440	43.45515232689159	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	2498	5.310712843080979	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	1371	2.9147267045092162	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	490	1.0417331037268533	RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	463	0.9843314837255778	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	455	0.9673235963177923	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	439	0.9333078215022217	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	188	0.39968535408295597	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	177	0.3762995088972511	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	176	0.3741735229712779	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	147	0.312519931118056	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	113	0.24023640963496823	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	109	0.23173246593107552	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	109	0.23173246593107552	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	85	0.18070880370771944	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	75	0.15944894444798774	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	73	0.15519697259604143	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCG	71	0.15094500074409506	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCG	67	0.14244105704020238	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTCTGCCGT	64	0.13606309926228288	No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT	59	0.12543316963241705	RNA PCR Primer, Index 30 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT	58	0.12330718370644386	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTACCGGATCTCGTATGCCGT	52	0.11055126815060483	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCCGGATCTCGTATGCCGT	49	0.10417331037268533	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.00425197185194634	0.0
5	0.0	0.0	0.0	0.0212598592597317	0.0
6	0.0	0.0	0.0	0.0212598592597317	0.0
7	0.0	0.0	0.0	0.02338584518570487	0.0
8	0.0	0.0	0.0	0.02338584518570487	0.0
9	0.0	0.0	0.0	0.02338584518570487	0.0
10	0.0	0.0	0.0	0.02551183111167804	0.0
11	0.0	0.0	0.0	0.02551183111167804	0.0
12	0.0	0.0	0.0	0.02551183111167804	0.0
13	0.0	0.0	0.0	0.02551183111167804	0.0
14	0.0	0.0	0.0	0.02551183111167804	0.0
15	0.0	0.0	0.0	0.02551183111167804	0.0
16	0.0	0.0	0.0	0.02551183111167804	0.0
17	0.0	0.0	0.0	0.02551183111167804	0.0
18	0.0	0.0	0.0	0.02763781703765121	0.0
19	0.0	0.0	0.0	0.04039373259349023	0.0
20	0.0	0.0	0.0	0.0425197185194634	0.0
21	0.0	0.0	0.0	0.04677169037140974	0.0
22	0.0	0.0	0.0	0.04889767629738291	0.0
23	0.0	0.0	0.0	0.05102366222335608	0.0
24	0.0	0.0	0.0	0.05314964814932925	0.0
25	0.0	0.0	0.0	0.05740162000127559	0.0
26	0.0	0.0	0.0	0.05740162000127559	0.0
27	0.0	0.0	0.0	0.07440950740906095	0.0
28	0.0	0.0	0.0	0.10204732444671216	0.0
29	0.0	0.0	0.0	0.1275591555583902	0.0
30	0.0	0.0	0.0	0.15307098667006824	0.0
31	0.0	0.0	0.0	0.18708676148563896	0.0
32	0.0	0.0	0.0	0.20409464889342432	0.0
33	0.0	0.0	0.0	0.24448838148691457	0.0
34	0.0	0.0	0.0	0.2912600718583243	0.0
35	0.0	0.0	0.0	0.37204753704530474	0.0
36	0.0	0.0	0.0	0.5017326785296682	0.0
37	0.0	0.0	0.0	0.7589769755724217	0.0
38	0.0	0.0	0.0	1.062992962986585	0.0
39	0.0	0.0	0.0	1.2989774007696069	0.0
40	0.0	0.0	0.0	1.6051193741097434	0.0
41	0.0	0.0	0.0	2.013308671896592	0.0
42	0.0	0.0	0.0	2.338584518570487	0.0
43	0.0	0.0	0.0	2.710632055615792	0.0
44	0.0	0.0	0.0	3.035907902289687	0.0
45	0.0	0.0	0.0	3.4462231860025088	0.0
46	0.0	0.0	0.0	3.8586644556413034	0.0
47	0.0	0.0	0.0	4.192444246019091	0.0
48	0.0	0.0	0.0	4.524098050470906	0.0
49	0.0	0.0	0.0	4.77496438973574	0.0
50	0.0	0.0	0.0	5.100240236409635	0.0
51	0.0	0.0	0.0	5.42551608308353	0.0
52	0.0	0.0	0.0	5.7869336904989686	0.0
53	0.0	0.0	0.0	6.154729255692327	0.0
54	0.0	0.0	0.0	6.490635031996089	0.0
55	0.0	0.0	0.0	6.801028977188171	0.0
56	0.0	0.0	0.0	7.160320598677637	0.0
57	0.0	0.0	0.0	7.413312923868444	0.0
58	0.0	0.0	0.0	7.649297361651466	0.0
59	0.0	0.0	0.0	7.830006165359185	0.0
60	0.0	0.0	0.0	8.070242574994154	0.0
61	0.0	0.0	0.0	8.248825392775899	0.0
62	0.0	0.0	0.0	8.397644407594022	0.0
63	0.0	0.0	0.0	8.567723281671876	0.0
64	0.0	0.0	0.0	8.788825817973086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	15	0.002211615	70.00001	32
CTATACA	30	1.0030635E-7	70.00001	1
ACAGAGA	15	0.002211615	70.00001	8
CTTTAAC	15	0.002211615	70.00001	63
CCAGTTG	15	0.002211615	70.00001	32
GAGCTAA	15	0.002211615	70.00001	1
ACTCCAG	15	0.002211615	70.00001	29
CATTCCC	15	0.002211615	70.00001	28
GACATTA	30	1.0030635E-7	70.00001	26
ACATTAC	25	2.7987971E-6	70.00001	27
TTCCCGG	15	0.002211615	70.00001	30
AACTCAC	15	0.002211615	70.00001	8
AATAGAC	15	0.002211615	70.00001	45
TACCGGA	15	0.002211615	70.00001	31
GCTAAAA	15	0.002211615	70.00001	3
TACGAAA	15	0.002211615	70.00001	52
TATTTAC	15	0.002211615	70.00001	3
ATAGACT	15	0.002211615	70.00001	46
AGTTGAC	15	0.002211615	70.00001	34
CGAAAGT	15	0.002211615	70.00001	54
>>END_MODULE