FastQCFastQC Report
Wed 25 May 2016
SRR1779866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376609
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTCG200.00694545552.50567635
CGCATAG200.00694910452.49870325
CCGTGTA352.0834443E-550.0054134
ACGATAC352.0850817E-549.99876824
ATTCGCG305.875055E-446.66551230
CGACGAT551.3110912E-744.5384422
GACGAAT503.475223E-641.99896223
ATCAGCG350.001255665739.99370211
ATCTCGG1300.037.6913842
TGGTCGC852.570232E-937.0579149
GTGTCGC400.00241609335.00378436
TCCGTGT501.7072182E-435.00378433
GTAGATC2000.033.2491838
TACACAG1206.548362E-1132.082545
TAGATCT1750.031.9992139
CGACGAG1109.0403773E-1031.81317322
CAATTTG450.00430254131.11447332
GACGCAT450.004305338531.11034623
GACCTAT450.004305338531.1103461
GTCGACG450.00430813731.10621520
Produced by FastQC (version 0.11.5)