##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779866_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376609
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.715537865531626	34.0	31.0	34.0	12.0	34.0
2	30.39404793831268	34.0	32.0	34.0	12.0	34.0
3	30.037041069119432	34.0	31.0	34.0	11.0	34.0
4	30.242222039303364	34.0	31.0	34.0	12.0	34.0
5	30.907625680745813	34.0	33.0	34.0	21.0	34.0
6	33.53450129975651	38.0	34.0	38.0	21.0	38.0
7	33.357702550921516	38.0	34.0	38.0	21.0	38.0
8	32.95387258403278	37.0	34.0	38.0	11.0	38.0
9	33.85215170110114	38.0	34.0	38.0	21.0	38.0
10-11	32.415959257479244	37.0	30.5	38.0	16.5	38.0
12-13	33.32857685291642	38.0	34.0	38.0	16.0	38.0
14-15	32.07600853936045	37.0	29.5	38.0	11.0	38.0
16-17	32.752577607014175	37.5	32.5	38.0	16.0	38.0
18-19	33.69359733835357	38.0	34.0	38.0	21.0	38.0
20-21	32.32871359951568	37.0	30.5	38.0	16.0	38.0
22-23	33.25230809672631	38.0	33.0	38.0	16.0	38.0
24-25	33.569621278301895	38.0	34.0	38.0	21.0	38.0
26-27	32.459155251202176	37.0	31.5	38.0	11.0	38.0
28-29	33.73044457248765	38.0	34.5	38.0	21.0	38.0
30-31	34.1115307918823	38.0	36.0	38.0	21.0	38.0
32-33	34.222165428866546	38.0	36.0	38.0	21.0	38.0
34-35	34.12959063644257	38.0	35.5	38.0	21.0	38.0
36-37	33.644639400545394	38.0	34.0	38.0	16.0	38.0
38-39	33.579064759472026	38.0	34.0	38.0	16.0	38.0
40-41	33.60648311644172	38.0	34.0	38.0	21.0	38.0
42-43	33.98516498543583	38.0	35.0	38.0	21.5	38.0
44-45	34.03083171140361	38.0	35.0	38.0	21.0	38.0
46-47	33.94619353228414	38.0	35.0	38.0	21.0	38.0
48-49	33.69335438080343	38.0	34.5	38.0	21.0	38.0
50-51	33.04676999222005	37.5	32.5	38.0	16.0	38.0
52-53	33.61439715991917	38.0	34.0	38.0	21.0	38.0
54-55	33.753164422517784	38.0	34.5	38.0	21.0	38.0
56-57	33.822432549408006	38.0	34.5	38.0	21.0	38.0
58-59	34.06104474401833	38.0	36.0	38.0	21.0	38.0
60-61	33.919950133958565	38.0	35.0	38.0	21.0	38.0
62-63	33.94693169839276	38.0	35.0	38.0	21.0	38.0
64-65	33.93515555921393	38.0	35.0	38.0	21.0	38.0
66-67	33.72941167099034	38.0	34.0	38.0	21.0	38.0
68-69	33.108333842260805	38.0	33.5	38.0	15.0	38.0
70-71	33.17510468416846	37.0	33.5	38.0	20.0	38.0
72-73	33.39250787952491	37.5	34.0	38.0	20.0	38.0
74-75	33.47335565533484	38.0	34.0	38.0	20.5	38.0
76	32.52359077982735	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	81.0
13	835.0
14	2450.0
15	4082.0
16	5435.0
17	6392.0
18	6723.0
19	6034.0
20	4746.0
21	3824.0
22	3263.0
23	2981.0
24	3025.0
25	3298.0
26	3826.0
27	4521.0
28	5163.0
29	6323.0
30	7931.0
31	9942.0
32	12627.0
33	17027.0
34	23654.0
35	35630.0
36	63623.0
37	133172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15207324326621	20.76695131277084	9.835425694621463	28.245549749341492
2	21.0061363376871	23.611225435398516	29.704282160012106	25.678356066902282
3	18.7802203346176	22.191716076886106	31.041478031592444	27.986585556903844
4	13.557296825089152	20.709011202600045	32.04809231855851	33.6855996537523
5	13.567386865422758	33.608862241741434	35.07218361749188	17.751567275343923
6	27.591151330725783	38.84874893454311	17.414544382751945	16.14555535197916
7	27.326197386554647	28.635841005228485	19.961443582029307	24.076518026187564
8	24.346521508231543	35.38343069569835	18.76898566117897	21.50106213489113
9	23.61954229933772	18.374944898533638	19.738009124271734	38.267503677856915
10-11	23.934976015634646	25.779508469873168	24.763442330645685	25.5220731838465
12-13	23.877283867353142	23.45151071801258	25.50456839852473	27.166637016109547
14-15	24.21884853594259	24.78615003684207	22.699096539500673	28.295904887714663
16-17	24.44567767323361	26.650619940866978	23.366174688038107	25.537527697861307
18-19	22.149052638287195	26.48762541539597	25.574053003397516	25.789268942919314
20-21	23.796696308372876	26.03562315292518	25.16283466406804	25.0048458746339
22-23	24.570370999776955	25.79805312742297	24.535188155197503	25.096387717602575
24-25	22.45686427581372	27.435993436041233	24.433619577166347	25.6735227109787
26-27	23.140302755923283	27.553789494088	24.932887599009046	24.37302015097967
28-29	22.591292433190297	27.107054849810936	25.80511535029953	24.49653736669924
30-31	22.970906165280173	26.590840898650853	25.665212461730867	24.773040474338107
32-33	21.90696704933638	27.337540177664604	25.61970853021108	25.135784242787935
34-35	22.30915582061968	27.291013060397535	25.7917957283193	24.60803539066348
36-37	23.75662436544853	26.061657887293833	25.388299687554362	24.793418059703278
38-39	23.3954924154413	25.844192916652837	26.269990374083047	24.490324293822816
40-41	22.55776254525612	26.13669705647672	26.141078287283943	25.164462110983216
42-43	21.319676320838006	26.080468392158945	26.675915907144702	25.92393937985834
44-45	21.02709125790798	24.92435557856863	27.98372267473895	26.064830488784445
46-47	21.07866761726856	25.061769208199074	27.701149883099863	26.158413291432502
48-49	21.05119912481944	25.54499638881808	27.969533307842635	25.43427117851984
50-51	22.25517751381091	25.47898392079755	27.107615548937947	25.1582230164536
52-53	19.27431529721684	26.70138367919781	27.47428422717248	26.550016796412873
54-55	19.646001014384613	26.160102819298803	27.088583393302407	27.105312773014184
56-57	19.794593302706005	26.275592851262715	26.985553814695805	26.944260031335475
58-59	19.632529064672546	26.021047655972	27.340177494994343	27.00624578436111
60-61	19.28948839605717	25.85002848367268	27.449761839314906	27.410721280955247
62-63	19.961203256695825	26.18607566028817	26.474860453876754	27.37786062913925
64-65	20.592617380149946	25.540789253531564	25.76706424138123	28.099529124937256
66-67	20.47276401767089	26.626503944347586	24.819181439988952	28.08155059799257
68-69	20.552775627740743	27.184596205343215	24.32008305707386	27.942545109842182
70-71	21.262825006372672	27.28367427139094	24.175137012490442	27.278363709745946
72-73	22.33103420806309	26.877725923909797	23.37088497513891	27.4203548928882
74-75	22.152355030371428	26.718358947123843	24.01885352009825	27.11043250240648
76	22.093319667125158	27.082193745054404	23.604214573630237	27.2202720141902
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.5
8	1.0
9	0.5
10	2.0
11	3.0
12	2.0
13	5.0
14	10.0
15	18.5
16	28.0
17	31.0
18	48.5
19	81.0
20	113.0
21	130.0
22	187.5
23	299.0
24	491.0
25	629.0
26	855.0
27	1263.0
28	1631.5
29	1818.0
30	2262.5
31	3131.0
32	4412.5
33	5270.0
34	5859.0
35	7405.5
36	9189.5
37	10016.0
38	11154.5
39	13938.5
40	16595.0
41	18612.0
42	19618.0
43	20284.5
44	22258.0
45	23678.5
46	23792.0
47	24102.5
48	23880.5
49	22349.5
50	21351.0
51	20594.0
52	18587.0
53	16192.5
54	15048.0
55	13782.0
56	11546.5
57	9743.0
58	8909.0
59	7953.5
60	6408.5
61	5335.0
62	4851.0
63	4564.0
64	3936.5
65	3351.5
66	2919.0
67	2731.0
68	2555.0
69	2190.5
70	1893.0
71	1784.0
72	1677.5
73	1523.5
74	1344.0
75	1212.0
76	1176.5
77	1063.5
78	907.0
79	828.0
80	738.5
81	602.5
82	497.5
83	439.0
84	377.0
85	293.5
86	219.5
87	167.0
88	145.0
89	105.5
90	69.5
91	43.0
92	35.0
93	34.5
94	29.0
95	16.5
96	9.0
97	10.5
98	12.0
99	13.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.6552737720022623E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.002655273772002262
7	0.004779492789604072
8	0.002389746394802036
9	0.008231348693207012
10-11	0.0025225100834021493
12-13	0.0
14-15	3.982910658003393E-4
16-17	1.3276368860011312E-4
18-19	0.0027880374606023754
20-21	0.0
22-23	0.001327636886001131
24-25	0.0018586916404015837
26-27	5.310547544004525E-4
28-29	0.004513965412403846
30-31	0.0
32-33	1.3276368860011312E-4
34-35	0.003319092215002828
36-37	0.016860988452214367
38-39	0.005708838609804864
40-41	6.638184430005655E-4
42-43	3.982910658003393E-4
44-45	0.003053564837802602
46-47	0.0014604005746012441
48-49	2.6552737720022623E-4
50-51	0.001194873197401018
52-53	0.010753858776609162
54-55	0.006903711807205881
56-57	0.010355567710808823
58-59	0.009293458202007917
60-61	0.020578371733017534
62-63	0.008231348693207012
64-65	0.020180080667217194
66-67	0.015267824189013008
68-69	1.3276368860011312E-4
70-71	2.6552737720022623E-4
72-73	0.004381201723803733
74-75	0.005708838609804864
76	0.0029208011492024883
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	376609.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.80103741310351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.62845086379812	42.75947053558962
2	8.473165514735724	9.456228517903732
3	5.305847302722707	8.882153515423322
4	3.461695902935812	7.726648903700334
5	1.9995835079904645	5.578941707000032
6	1.1672106787028818	3.9078940050764053
7	0.6408586502672594	2.5032404264052044
8	0.4188827274423987	1.8699272596572898
9	0.32179215312088694	1.6160702377987568
>10	1.5561863955578898	14.768219925428856
>50	0.025838778601345702	0.898559743719673
>100	4.875241245532818E-4	0.03264522229678585
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001327636886001131	0.0
2	0.0	0.0	0.0	0.00212421901760181	0.0
3	0.0	0.0	0.0	0.00212421901760181	0.0
4	0.0	0.0	0.0	0.003717383280803167	0.0
5	0.0	0.0	0.0	0.004779492789604072	0.0
6	0.0	0.0	0.0	0.004779492789604072	0.0
7	0.0	0.0	0.0	0.004779492789604072	0.0
8	0.0	0.0	0.0	0.00637265705280543	0.0
9	0.0	0.0	0.0	0.006638184430005655	0.0
10	0.0	0.0	0.0	0.008231348693207014	0.0
11	0.0	0.0	0.0	0.00849687607040724	0.0
12	0.0	0.0	0.0	0.009027930824807693	0.0
13	0.0	0.0	0.0	0.010355567710808823	0.0
14	0.0	0.0	0.0	0.010355567710808823	0.0
15	0.0	0.0	0.0	0.010355567710808823	0.0
16	0.0	0.0	0.0	0.010621095088009049	0.0
17	0.0	0.0	0.0	0.011152149842409502	0.0
18	0.0	0.0	0.0	0.011683204596809955	0.0
19	0.0	0.0	0.0	0.012479786728410634	0.0
20	0.0	0.0	0.0	0.01699375214081448	0.0
21	0.0	0.0	0.0	0.017790334272415158	0.0
22	0.0	0.0	0.0	0.025756155588421946	0.0
23	0.0	0.0	0.0	0.029208011492024885	0.0
24	0.0	0.0	0.0	0.030801175755226243	0.0
25	0.0	0.0	0.0	0.03478408641322964	0.0
26	0.0	0.0	0.0	0.039829106580033934	0.0
27	0.0	0.0	0.0	0.0820479595548699	0.0
28	0.0	0.0	0.0	0.10567989612569004	0.0
29	0.0	0.0	0.0	0.13223263384571265	0.0
30	0.0	0.0	0.0	0.17392043206614818	0.0
31	0.0	0.0	0.0	0.2177324493041855	0.0
32	0.0	0.0	0.0	0.27057239736703054	0.0
33	0.0	0.0	0.0	0.3159775788682692	0.0
34	0.0	0.0	0.0	0.38793549808953054	0.0
35	0.0	0.0	0.0	0.4872427371624151	0.0
36	0.0	0.0	0.0	0.6776258666149774	0.0
37	0.0	0.0	0.0	1.0650303099501075	0.0
38	0.0	0.0	0.0	1.6024577214033653	0.0
39	0.0	0.0	0.0	2.1401506602338234	0.0
40	0.0	0.0	0.0	2.815917835208399	0.0
41	0.0	0.0	0.0	3.7402186352423867	0.0
42	0.0	0.0	0.0	4.7927691584640835	0.0
43	0.0	0.0	0.0	5.893645664336221	0.0
44	0.0	0.0	0.0	6.852465023406238	0.0
45	0.0	0.0	0.0	7.702152630446962	0.0
46	0.0	0.0	0.0	8.5696305717601	0.0
47	0.0	0.0	0.0	9.430470328643235	0.0
48	0.0	0.0	0.0	10.237673555331922	0.0
49	0.0	0.0	0.0	10.944507433438925	0.0
50	0.0	0.0	0.0	11.704446786985972	0.0
51	0.0	0.0	0.0	12.5599759963251	0.0
52	0.0	0.0	0.0	13.62819263480161	0.0
53	0.0	0.0	0.0	14.771022466271384	0.0
54	0.0	0.0	0.0	15.76117405585103	0.0
55	0.0	0.0	0.0	16.653080515866588	0.0
56	0.0	0.0	0.0	17.399743500553626	0.0
57	0.0	0.0	0.0	18.094628646686616	0.0
58	0.0	0.0	0.0	18.75579181591518	0.0
59	0.0	0.0	0.0	19.33145516968527	0.0
60	0.0	0.0	0.0	19.877910511963336	0.0
61	0.0	0.0	0.0	20.453042810979024	0.0
62	0.0	0.0	0.0	21.050479409679536	0.0
63	0.0	0.0	0.0	21.815729310770585	0.0
64	0.0	0.0	0.0	22.556019638404816	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGTCG	20	0.006945455	52.505676	35
CGCATAG	20	0.006949104	52.498703	25
CCGTGTA	35	2.0834443E-5	50.00541	34
ACGATAC	35	2.0850817E-5	49.998768	24
ATTCGCG	30	5.875055E-4	46.665512	30
CGACGAT	55	1.3110912E-7	44.53844	22
GACGAAT	50	3.475223E-6	41.998962	23
ATCAGCG	35	0.0012556657	39.993702	11
ATCTCGG	130	0.0	37.69138	42
TGGTCGC	85	2.570232E-9	37.05791	49
GTGTCGC	40	0.002416093	35.003784	36
TCCGTGT	50	1.7072182E-4	35.003784	33
GTAGATC	200	0.0	33.24918	38
TACACAG	120	6.548362E-11	32.08254	5
TAGATCT	175	0.0	31.99921	39
CGACGAG	110	9.0403773E-10	31.813173	22
CAATTTG	45	0.004302541	31.114473	32
GACGCAT	45	0.0043053385	31.110346	23
GACCTAT	45	0.0043053385	31.110346	1
GTCGACG	45	0.004308137	31.106215	20
>>END_MODULE