FastQCFastQC Report
Wed 25 May 2016
SRR1779866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376609
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT291487.739591990632194No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG34920.92722160118319No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC22860.6069955842797171No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT8260.21932561356738686Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6710.1781688701013518No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT150.002221615570.0168764
CGCGAAT150.002221615570.0168763
ATATACG150.00222512969.988983
AGACCGT207.927887E-569.9889825
ATATCGT308.377016E-658.3241537
TGCGTAT252.3845569E-456.02093555
GGTCGAT252.390825E-455.99118410
GAGACCG252.390825E-455.99118424
TATGCCG41500.053.6301143
CGTATGC41700.053.5407341
GCCGTCT41300.053.4803646
ATGCCGT41650.053.35294344
TCGTATG41900.053.28516840
TGCCGTC41850.053.27935845
CCGTCTT41500.053.22262247
TCTCGTA40350.053.0771538
GTATGCC42100.053.03203242
ATCTCGT40550.052.98795737
CTCGTAT41750.052.97369439
CGTCTTC41450.052.95606248