##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779866_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376609
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.571985799595865	34.0	34.0	34.0	34.0	34.0
2	33.65466571430847	34.0	34.0	34.0	34.0	34.0
3	33.76851588783062	34.0	34.0	34.0	34.0	34.0
4	33.57478180287779	34.0	34.0	34.0	34.0	34.0
5	33.63625404597341	34.0	34.0	34.0	34.0	34.0
6	37.316285590625824	38.0	38.0	38.0	37.0	38.0
7	37.35506055351837	38.0	38.0	38.0	37.0	38.0
8	37.27830720986487	38.0	38.0	38.0	36.0	38.0
9	37.188625338215495	38.0	38.0	38.0	36.0	38.0
10-11	37.14004577691983	38.0	38.0	38.0	36.0	38.0
12-13	37.304395274674796	38.0	38.0	38.0	36.5	38.0
14-15	37.318672681746854	38.0	38.0	38.0	36.5	38.0
16-17	37.2574487067489	38.0	38.0	38.0	36.0	38.0
18-19	37.133752778080186	38.0	38.0	38.0	36.0	38.0
20-21	37.13571635303458	38.0	38.0	38.0	36.0	38.0
22-23	37.135202557559694	38.0	38.0	38.0	36.0	38.0
24-25	37.30907917760861	38.0	38.0	38.0	36.5	38.0
26-27	37.22597441909248	38.0	38.0	38.0	36.0	38.0
28-29	36.79355379186371	38.0	37.0	38.0	35.0	38.0
30-31	37.071262237493	38.0	38.0	38.0	36.0	38.0
32-33	37.14336354149795	38.0	38.0	38.0	36.0	38.0
34-35	37.130862512579355	38.0	38.0	38.0	36.0	38.0
36-37	37.103995124917354	38.0	38.0	38.0	36.0	38.0
38-39	36.803110122169144	38.0	37.5	38.0	35.0	38.0
40-41	36.98738744958299	38.0	37.5	38.0	35.5	38.0
42-43	37.22674312084948	38.0	38.0	38.0	36.5	38.0
44-45	37.01309581024351	38.0	38.0	38.0	35.5	38.0
46-47	37.12834664068039	38.0	38.0	38.0	36.0	38.0
48-49	36.81268902230165	38.0	37.0	38.0	35.0	38.0
50-51	36.97945216391536	38.0	37.5	38.0	35.5	38.0
52-53	36.903709151932105	38.0	38.0	38.0	35.5	38.0
54-55	36.91340886702123	38.0	37.5	38.0	35.5	38.0
56-57	36.78688241651156	38.0	37.0	38.0	35.0	38.0
58-59	36.88351446725915	38.0	37.5	38.0	35.5	38.0
60-61	36.58294544208981	38.0	37.0	38.0	34.0	38.0
62-63	36.41915753473762	38.0	37.0	38.0	34.0	38.0
64-65	36.753617146695916	38.0	37.0	38.0	34.5	38.0
66-67	36.56142444816773	38.0	37.0	38.0	34.5	38.0
68-69	36.656334553874174	38.0	37.0	38.0	34.0	38.0
70-71	36.06274013632176	38.0	37.0	38.0	32.5	38.0
72-73	35.00810787846281	38.0	37.0	38.0	24.0	38.0
74-75	34.84309323462796	38.0	37.0	38.0	23.5	38.0
76	34.57540313694044	38.0	36.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	7.0
22	20.0
23	21.0
24	71.0
25	132.0
26	325.0
27	568.0
28	996.0
29	1665.0
30	2714.0
31	4468.0
32	7222.0
33	11285.0
34	19065.0
35	37499.0
36	59783.0
37	230767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.144362324461895	16.474413810325863	10.656617447810124	32.724606417402114
2	20.63413248222958	18.74304650180957	36.67437581151805	23.948445204442802
3	18.74331202918677	19.738243111556017	35.01987472418344	26.49857013507378
4	13.23760186294008	23.422435470209155	34.2663080276892	29.07365463916157
5	12.803730128594909	31.544386884009672	40.81607184108718	14.83581114630824
6	27.169026762504352	41.46342758670133	17.729262975659104	13.638282675135219
7	26.33898818137644	27.63874469277155	20.10281220045193	25.919454925400082
8	23.542188317326456	39.40054539323277	17.855122952452014	19.20214333698876
9	22.6566545143637	17.17457628468783	18.628338674859073	41.540430526089395
10-11	23.36375391984791	27.13291503920512	27.79354715367928	21.709783887267697
12-13	23.444739769893975	21.016359141709305	28.069961153344714	27.468939935052006
14-15	21.42075733718525	21.354375492885193	21.870560714162433	35.35430645576712
16-17	26.20635725646493	28.16050598896999	22.38103709682987	23.25209965773521
18-19	26.206622783842125	24.452017875303035	23.632733152951737	25.708626187903107
20-21	21.202493833126663	23.853120876027923	23.238690525186602	31.705694765658816
22-23	21.431643959650458	28.45948981569745	23.386854801664324	26.72201142298777
24-25	25.981593544464122	28.22066504340068	23.381270130401187	22.41647128173401
26-27	25.97402080141473	29.138974373952824	23.428675363573362	21.458329461059083
28-29	21.45753287892748	28.914736503907235	24.082271002551717	25.545459614613563
30-31	25.67875435796808	24.64957024394	28.115233571157354	21.556441826934567
32-33	25.39849021133324	28.659033639663416	24.90859220039882	21.03388394860452
34-35	22.001067420056344	28.61389398553938	28.620930461035186	20.764108133369092
36-37	21.143812282765413	27.971981551157832	29.505800445554943	21.378405720521815
38-39	20.57677856875433	24.540704019287908	29.103393705408	25.77912370654976
40-41	20.744060816390476	22.434806390712914	29.982953142383746	26.838179650512867
42-43	25.406190505272047	22.302573756867204	29.37569203072683	22.915543707133924
44-45	20.460875868606433	26.812556258613046	30.698018369183956	22.02854950359657
46-47	24.419490771596006	22.20193888090832	26.848535218223674	26.530035129272004
48-49	23.882461651208548	21.95991067659031	26.343369383100246	27.814258289100895
50-51	19.799716384378836	22.58382328728563	30.83045203231306	26.78600829602248
52-53	18.567034788290226	22.687951852028917	35.532767074097094	23.212246285583763
54-55	18.240224205716714	22.512751036021676	31.66905748592073	27.577967272340874
56-57	22.445238749885437	22.690703040011474	26.713235811752266	28.150822398350822
58-59	18.275871228219295	22.499070335741607	34.73690501487463	24.488153421164473
60-61	22.567030421831152	26.848026306178525	26.767147556571963	23.81779571541836
62-63	18.216804111389983	33.033345285048405	24.45413861333546	24.295711990226152
64-65	18.465346271716673	34.16243088640673	22.990859212755673	24.381363629120923
66-67	18.275568517611	33.95978841038186	22.345069571442043	25.419573500565097
68-69	18.161250104560427	34.15481316545244	22.387483021378127	25.296453708609008
70-71	18.51721532735744	33.10433559896805	22.652717898602777	25.72573117507173
72-73	19.582027001724956	31.321947831721914	22.37502041661853	26.7210047499346
74-75	20.022625786226154	31.462191434865222	22.18641576475556	26.328767014153065
76	20.557570864824385	31.439709795465287	21.84022816959757	26.162491170112755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	1.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	1.0
15	7.5
16	24.0
17	35.0
18	44.0
19	94.0
20	155.0
21	175.0
22	209.5
23	296.5
24	548.5
25	748.0
26	961.5
27	1281.5
28	1681.5
29	1975.0
30	2276.5
31	3023.5
32	4165.5
33	4862.0
34	5584.0
35	6983.5
36	8646.0
37	9631.0
38	15252.5
39	24210.5
40	27204.5
41	25011.5
42	23161.0
43	22238.5
44	21819.5
45	21976.0
46	21629.0
47	21564.0
48	20911.0
49	19631.0
50	18939.0
51	18256.0
52	15870.0
53	13365.5
54	12564.0
55	11568.0
56	9665.5
57	8006.0
58	7253.0
59	6258.5
60	4996.0
61	4304.5
62	3881.0
63	3670.0
64	3153.5
65	2840.5
66	2660.0
67	2487.0
68	2321.0
69	2115.5
70	1862.0
71	1648.0
72	1567.0
73	1442.0
74	1321.0
75	1244.0
76	1189.5
77	1021.5
78	821.0
79	734.0
80	672.0
81	522.5
82	385.0
83	335.0
84	319.5
85	265.5
86	162.5
87	98.0
88	83.0
89	53.5
90	34.0
91	21.0
92	13.0
93	8.0
94	1.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.051777838554044116
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.310547544004525E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.011417677219609727
52-53	0.0273493198516233
54-55	0.04487412674683824
56-57	0.047662164207440606
58-59	0.03425303165882918
60-61	0.03173052157542704
62-63	0.024959573456821264
64-65	0.014604005746012443
66-67	0.033323685838628395
68-69	0.008629639759007351
70-71	0.010753858776609162
72-73	0.020578371733017534
74-75	0.012612550417010746
76	0.012479786728410632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	376609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.240838641668148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.28611167488449	7.847927160529887
2	13.512255106791942	6.010477710304321
3	12.688482706748964	8.466074894652014
4	10.879765045785032	9.679003953702646
5	8.199520063036497	9.118210133055769
6	5.5706116211602055	7.433704452097533
7	3.7607000871527325	5.854878667264988
8	2.177624431417963	3.8745754881057013
9	1.3347500626783348	2.6717364693886765
>10	6.333496495982617	25.251653571741517
>50	0.2244481321856234	3.2195194485527425
>100	0.026265206957892095	0.9009343908403676
>500	0.0023877460870810998	0.39749448366873863
>1k	0.0023877460870810998	1.5342171854629072
>5k	0.0	0.0
>10k+	0.0011938730435405499	7.739591990632194
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	29148	7.739591990632194	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	3492	0.92722160118319	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	2286	0.6069955842797171	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	826	0.21932561356738686	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	671	0.1781688701013518	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002389746394802036	0.0
2	0.0	0.0	0.0	0.003982910658003393	0.0
3	0.0	0.0	0.0	0.003982910658003393	0.0
4	0.0	0.0	0.0	0.00637265705280543	0.0
5	0.0	0.0	0.0	0.008231348693207014	0.0
6	0.0	0.0	0.0	0.008231348693207014	0.0
7	0.0	0.0	0.0	0.008231348693207014	0.0
8	0.0	0.0	0.0	0.010090040333608597	0.0
9	0.0	0.0	0.0	0.010621095088009049	0.0
10	0.0	0.0	0.0	0.01274531410561086	0.0
11	0.0	0.0	0.0	0.01327636886001131	0.0
12	0.0	0.0	0.0	0.014338478368812217	0.0
13	0.0	0.0	0.0	0.01566611525481335	0.0
14	0.0	0.0	0.0	0.01566611525481335	0.0
15	0.0	0.0	0.0	0.01566611525481335	0.0
16	0.0	0.0	0.0	0.015931642632013573	0.0
17	0.0	0.0	0.0	0.016462697386414028	0.0
18	0.0	0.0	0.0	0.017259279518014706	0.0
19	0.0	0.0	0.0	0.018055861649615385	0.0
20	0.0	0.0	0.0	0.02389746394802036	0.0
21	0.0	0.0	0.0	0.024959573456821267	0.0
22	0.0	0.0	0.0	0.02549062821122172	0.0
23	0.0	0.0	0.0	0.028942484114824658	0.0
24	0.0	0.0	0.0	0.028942484114824658	0.0
25	0.0	0.0	0.0	0.0334564495272285	0.0
26	0.0	0.0	0.0	0.0385014696940328	0.0
27	0.0	0.0	0.0	0.08417217857247171	0.0
28	0.0	0.0	0.0	0.1083351698976923	0.0
29	0.0	0.0	0.0	0.1356844897493156	0.0
30	0.0	0.0	0.0	0.17949650698735292	0.0
31	0.0	0.0	0.0	0.228884599146595	0.0
32	0.0	0.0	0.0	0.2814590198322398	0.0
33	0.0	0.0	0.0	0.3300505298598812	0.0
34	0.0	0.0	0.0	0.40519477760754524	0.0
35	0.0	0.0	0.0	0.5114057284876358	0.0
36	0.0	0.0	0.0	0.7145341720458088	0.0
37	0.0	0.0	0.0	1.129022407855362	0.0
38	0.0	0.0	0.0	1.6903472832566402	0.0
39	0.0	0.0	0.0	2.2399889540611087	0.0
40	0.0	0.0	0.0	2.952929961843716	0.0
41	0.0	0.0	0.0	3.913076957799734	0.0
42	0.0	0.0	0.0	5.0205916481018775	0.0
43	0.0	0.0	0.0	6.169794136624457	0.0
44	0.0	0.0	0.0	7.170566821292109	0.0
45	0.0	0.0	0.0	8.05105560408806	0.0
46	0.0	0.0	0.0	8.969514801823642	0.0
47	0.0	0.0	0.0	9.864873117742805	0.0
48	0.0	0.0	0.0	10.703939629695519	0.0
49	0.0	0.0	0.0	11.45431999766336	0.0
50	0.0	0.0	0.0	12.238422342535626	0.0
51	0.0	0.0	0.0	13.10988319450677	0.0
52	0.0	0.0	0.0	14.199607550536498	0.0
53	0.0	0.0	0.0	15.341640799874671	0.0
54	0.0	0.0	0.0	16.34028926552472	0.0
55	0.0	0.0	0.0	17.233523362426283	0.0
56	0.0	0.0	0.0	17.98231056613092	0.0
57	0.0	0.0	0.0	18.69631368342233	0.0
58	0.0	0.0	0.0	19.364646091835297	0.0
59	0.0	0.0	0.0	19.959958471518206	0.0
60	0.0	0.0	0.0	20.50641381379627	0.0
61	0.0	0.0	0.0	21.094025899540373	0.0
62	0.0	0.0	0.0	21.705269921855294	0.0
63	0.0	0.0	0.0	22.484858301315157	0.0
64	0.0	0.0	0.0	23.257542968967815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATT	15	0.0022216155	70.01687	64
CGCGAAT	15	0.0022216155	70.01687	63
ATATACG	15	0.002225129	69.98898	3
AGACCGT	20	7.927887E-5	69.98898	25
ATATCGT	30	8.377016E-6	58.32415	37
TGCGTAT	25	2.3845569E-4	56.020935	55
GGTCGAT	25	2.390825E-4	55.991184	10
GAGACCG	25	2.390825E-4	55.991184	24
TATGCCG	4150	0.0	53.63011	43
CGTATGC	4170	0.0	53.54073	41
GCCGTCT	4130	0.0	53.48036	46
ATGCCGT	4165	0.0	53.352943	44
TCGTATG	4190	0.0	53.285168	40
TGCCGTC	4185	0.0	53.279358	45
CCGTCTT	4150	0.0	53.222622	47
TCTCGTA	4035	0.0	53.07715	38
GTATGCC	4210	0.0	53.032032	42
ATCTCGT	4055	0.0	52.987957	37
CTCGTAT	4175	0.0	52.973694	39
CGTCTTC	4145	0.0	52.956062	48
>>END_MODULE