FastQCFastQC Report
Wed 25 May 2016
SRR1779865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40907
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAG309.9978934E-870.029
TTGTAGA150.00220955370.036
GGATAGT150.00220955370.031
ATATCTA150.00220955370.033
GAGCGGC150.00220955370.026
AGGATAG150.00220955370.030
TTCGCGG150.00220955370.017
GGTGGTA207.8376994E-569.9999947
CTCTCGG207.8376994E-569.9999942
GCCGCTG352.9050898E-760.014
CTTACGC352.9050898E-760.012
TCTTACG308.237101E-658.33333211
TGTGTAG308.237101E-658.33333235
AGATCCC252.3637828E-455.99999640
TTAGATC252.3637828E-455.99999638
GAATAGA252.3637828E-455.99999626
TGTAGAG252.3637828E-455.99999637
GACGAGA252.3637828E-455.99999623
TCGCCCA200.006904437752.49999618
GAGGATA200.006904437752.49999629
Produced by FastQC (version 0.11.5)