##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779865_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40907
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	20.72227247170411	21.0	12.0	27.0	12.0	34.0
2	20.341995257535384	21.0	12.0	31.0	12.0	34.0
3	20.82719338988437	23.0	11.0	31.0	11.0	34.0
4	21.50443689344122	23.0	12.0	32.0	12.0	34.0
5	20.313173784437872	21.0	11.0	31.0	11.0	34.0
6	22.51617082650891	22.0	12.0	34.0	11.0	37.0
7	22.81570391375559	23.0	12.0	34.0	11.0	37.0
8	22.86982668003031	23.0	11.0	34.0	11.0	37.0
9	23.28650353240277	24.0	11.0	34.0	11.0	37.0
10-11	22.066040042046595	22.0	11.5	32.5	11.0	37.0
12-13	21.77168944190481	21.0	11.0	32.0	11.0	37.0
14-15	21.237868824406583	21.0	11.0	29.5	11.0	37.0
16-17	21.164531742733516	21.0	11.0	29.5	11.0	37.0
18-19	20.86933776615249	21.0	11.0	31.0	10.0	37.0
20-21	21.157295817341776	21.0	11.0	29.5	10.5	36.5
22-23	21.17759796611827	21.0	11.0	32.0	10.5	37.0
24-25	21.425880656122423	21.0	11.0	31.0	10.0	37.0
26-27	20.809506930354218	21.0	11.0	31.0	10.0	37.0
28-29	20.64914317842912	21.0	11.0	31.0	10.0	37.0
30-31	20.617950473024177	21.0	11.0	31.0	10.5	37.0
32-33	20.820678612462416	21.0	11.0	31.0	11.0	37.0
34-35	21.005426944043805	21.0	11.0	31.0	11.0	37.0
36-37	20.803578849585648	21.0	11.0	31.0	11.0	37.0
38-39	20.67103429730853	21.0	11.0	31.0	11.0	37.0
40-41	20.704231549612537	21.0	11.0	28.5	11.0	37.0
42-43	20.81570391375559	21.0	11.0	31.0	11.0	37.0
44-45	21.002249003837974	21.0	11.0	31.0	11.0	37.0
46-47	20.83087246681497	21.0	11.0	30.5	11.0	37.0
48-49	20.48948835162686	21.0	11.0	27.0	11.0	37.0
50-51	20.288165839587357	21.0	11.0	27.0	11.0	37.0
52-53	20.704610457867844	21.0	11.0	29.0	11.0	37.0
54-55	20.559134133522377	21.0	11.0	30.0	11.0	37.0
56-57	20.59249028283668	21.0	11.0	30.0	11.0	37.0
58-59	20.346187694037695	21.0	11.0	29.0	10.0	37.0
60-61	20.741364558632995	21.0	11.0	30.0	11.0	37.0
62-63	20.35562373187963	21.0	11.0	27.5	11.0	37.0
64-65	20.34296086244408	20.0	11.0	29.0	10.5	37.0
66-67	20.747891558901898	21.0	11.0	29.5	11.0	37.0
68-69	20.630283325592195	21.0	11.0	29.5	10.5	37.0
70-71	20.81756178649131	21.0	11.0	30.0	11.0	37.0
72-73	20.178086879996087	20.0	11.0	27.0	10.5	37.0
74-75	20.04704573789327	20.0	11.0	27.0	10.0	37.0
76	19.95545994573056	20.0	11.0	27.0	11.0	36.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
10	1.0
11	1.0
12	58.0
13	604.0
14	2052.0
15	3301.0
16	4285.0
17	5017.0
18	5198.0
19	4525.0
20	3473.0
21	2396.0
22	1542.0
23	1014.0
24	766.0
25	522.0
26	426.0
27	334.0
28	314.0
29	235.0
30	238.0
31	253.0
32	251.0
33	330.0
34	398.0
35	546.0
36	916.0
37	1911.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.34688439631359	20.25325738871098	9.255139707140588	40.144718507834845
2	11.885496369814458	23.484978121103968	26.902486127068713	37.727039382012855
3	11.073899332632557	21.336201628083213	40.440511403916204	27.14938763536803
4	9.428704133766837	46.67905248490478	15.593908133082357	28.29833524824602
5	10.284303419952575	31.96274476251008	33.651942210379644	24.1010096071577
6	12.298628596572714	41.1323245410321	12.049282518884299	34.519764343510886
7	11.898010071872097	38.33911895565443	11.51909255365961	38.24377841881387
8	10.193854352555798	43.66000928936368	10.340528515901925	35.8056078421786
9	15.435388451571896	23.355986896787755	11.428641275118565	49.77998337652178
10-11	11.96890431721508	36.85767369090109	18.73930474746981	32.434117244414026
12-13	12.299850881267265	25.846432150976607	23.658542547730217	38.195174420025914
14-15	26.172782164421736	30.00220011245019	15.81880851687975	28.006209206248318
16-17	27.375510303859972	25.192509839391793	14.404625125284182	33.02735473146405
18-19	16.311588622835004	25.880972461589234	20.3280652219085	37.47937369366727
20-21	26.180115872589045	24.224460361307358	15.83958735668712	33.75583640941648
22-23	27.53263579914927	23.936586319855277	14.380530973451327	34.15024690754412
24-25	16.470487575324213	33.299108943566424	13.853544057790327	36.37685942331903
26-27	24.04630071015609	33.415227408847	15.289746128365907	27.248725752630996
28-29	18.11959125800616	31.875519483694326	19.461692661223292	30.54319659707622
30-31	20.40359840614076	28.578238443298215	19.736230962915883	31.281932187645147
32-33	15.86525533527269	31.332045860121738	21.116190383064023	31.686508421541546
34-35	15.184813963721702	36.32718916540361	18.450838507798366	30.037158363076323
36-37	23.103629539981174	25.80653048251244	20.10366621434946	30.986173763156934
38-39	23.811561075187328	22.380178220000243	24.172157097629846	29.636103607182584
40-41	17.679125821986457	31.162148287579143	20.24347911115457	30.91524677927983
42-43	12.08717334441538	30.118317158432546	22.81761067787909	34.97689881927298
44-45	12.150732148532036	25.308626885374142	26.926931820959737	35.61370914513409
46-47	16.357836067176766	28.622240692302054	22.089128999926665	32.930794240594516
48-49	17.97247414867871	29.24560588652309	20.480602341897473	32.301317622900726
50-51	27.139609357811622	28.24944386046398	14.985210355195932	29.625736426528466
52-53	10.89733403415272	33.40708235035265	19.952572455353323	35.7430111601413
54-55	15.42946547530283	27.426629670826664	19.256579188617668	37.88732566525284
56-57	18.162037942499513	30.151085468413847	16.84554077840798	34.841335810678665
58-59	15.774355213299106	30.90820193130424	18.22271116000489	35.094731695391765
60-61	12.195897210200739	28.119269419790214	19.57016063962444	40.114672730384605
62-63	15.328386852302314	29.484531041816915	18.164260655918053	37.02282144996272
64-65	15.912174965464127	28.08836293842223	18.168926270492307	37.83053582562134
66-67	12.669462000171142	38.01205334767673	13.90291309609672	35.415571556055404
68-69	14.063607695504437	41.20932847678881	12.012613978047767	32.71444984965898
70-71	14.397291417116875	41.9989243894688	11.748600484024738	31.85518370938959
72-73	16.938027136046937	36.24862486248625	13.324776922136659	33.48857107933016
74-75	14.859859921037513	34.36579433083571	17.668773147865203	33.10557260026158
76	12.2992299229923	39.59907101821293	14.428553966507762	33.673145092287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	0.0
17	0.0
18	2.5
19	5.0
20	6.5
21	8.0
22	15.0
23	22.0
24	25.0
25	28.0
26	34.5
27	53.0
28	75.0
29	85.0
30	105.0
31	154.5
32	221.0
33	258.0
34	305.0
35	397.0
36	511.5
37	581.0
38	693.0
39	928.0
40	1145.0
41	1360.5
42	1482.0
43	1655.5
44	1935.0
45	2156.5
46	2272.0
47	2386.5
48	2535.0
49	2619.5
50	2670.0
51	2650.5
52	2510.0
53	2382.0
54	2375.0
55	2234.5
56	1986.0
57	1741.5
58	1605.0
59	1531.0
60	1314.0
61	1077.5
62	984.0
63	900.5
64	697.0
65	531.5
66	442.0
67	398.0
68	353.0
69	261.5
70	211.5
71	208.0
72	174.5
73	123.5
74	85.5
75	65.0
76	66.0
77	52.0
78	41.5
79	46.0
80	41.0
81	28.5
82	20.5
83	20.0
84	20.0
85	16.0
86	10.0
87	8.0
88	8.5
89	7.5
90	7.0
91	7.0
92	6.0
93	3.5
94	1.5
95	2.5
96	3.0
97	4.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0024445693891021096
8	0.0
9	0.0024445693891021096
10-11	0.0024445693891021096
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0012222846945510548
20-21	0.0
22-23	0.0024445693891021096
24-25	0.0012222846945510548
26-27	0.0012222846945510548
28-29	0.0024445693891021096
30-31	0.0
32-33	0.0
34-35	0.0024445693891021096
36-37	0.015889701029163712
38-39	0.006111423472755274
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.006111423472755274
54-55	0.003666854083653164
56-57	0.007333708167306328
58-59	0.004889138778204219
60-61	0.019556555112816877
62-63	0.006111423472755274
64-65	0.01833427041826582
66-67	0.013445131640061602
68-69	0.0
70-71	0.0
72-73	0.004889138778204219
74-75	0.003666854083653164
76	0.004889138778204219
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	40907.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.73161072677048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.16780389183725	94.95929791967144
2	1.005812484205206	1.9458772337252794
3	0.49027040687389434	1.4227393844574279
4	0.1844831943391458	0.713814261617816
5	0.08339651250947687	0.4033539492018481
6	0.032853171594642405	0.19067641234996455
7	0.012635835228708616	0.08555992861857384
8	0.010108668182966895	0.07822622045126751
9	0.0025271670457417236	0.02200112450191899
>10	0.010108668182966895	0.178453565404454
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0024445693891021096	0.0
5	0.0	0.0	0.0	0.009778277556408439	0.0
6	0.0	0.0	0.0	0.009778277556408439	0.0
7	0.0	0.0	0.0	0.009778277556408439	0.0
8	0.0	0.0	0.0	0.009778277556408439	0.0
9	0.0	0.0	0.0	0.012222846945510549	0.0
10	0.0	0.0	0.0	0.012222846945510549	0.0
11	0.0	0.0	0.0	0.012222846945510549	0.0
12	0.0	0.0	0.0	0.012222846945510549	0.0
13	0.0	0.0	0.0	0.012222846945510549	0.0
14	0.0	0.0	0.0	0.012222846945510549	0.0
15	0.0	0.0	0.0	0.012222846945510549	0.0
16	0.0	0.0	0.0	0.014667416334612658	0.0
17	0.0	0.0	0.0	0.014667416334612658	0.0
18	0.0	0.0	0.0	0.019556555112816877	0.0
19	0.0	0.0	0.0	0.029334832669225316	0.0
20	0.0	0.0	0.0	0.03422397144742954	0.0
21	0.0	0.0	0.0	0.03422397144742954	0.0
22	0.0	0.0	0.0	0.08311535922947173	0.0
23	0.0	0.0	0.0	0.10756105312049283	0.0
24	0.0	0.0	0.0	0.12467303884420759	0.0
25	0.0	0.0	0.0	0.12467303884420759	0.0
26	0.0	0.0	0.0	0.13445131640061603	0.0
27	0.0	0.0	0.0	0.13689588578971815	0.0
28	0.0	0.0	0.0	0.1515633021243308	0.0
29	0.0	0.0	0.0	0.15889701029163714	0.0
30	0.0	0.0	0.0	0.16867528784804556	0.0
31	0.0	0.0	0.0	0.18089813479355613	0.0
32	0.0	0.0	0.0	0.2053438286845772	0.0
33	0.0	0.0	0.0	0.23956780013200674	0.0
34	0.0	0.0	0.0	0.25912435524482363	0.0
35	0.0	0.0	0.0	0.3031266042486616	0.0
36	0.0	0.0	0.0	0.3593517001980101	0.0
37	0.0	0.0	0.0	0.47180189209670714	0.0
38	0.0	0.0	0.0	0.5989195003300168	0.0
39	0.0	0.0	0.0	0.7187034003960202	0.0
40	0.0	0.0	0.0	0.8800449800767595	0.0
41	0.0	0.0	0.0	1.1098345026523577	0.0
42	0.0	0.0	0.0	1.3102891925587308	0.0
43	0.0	0.0	0.0	1.5107438824651038	0.0
44	0.0	0.0	0.0	1.623194074363801	0.0
45	0.0	0.0	0.0	1.8212041948810718	0.0
46	0.0	0.0	0.0	2.002102329674628	0.0
47	0.0	0.0	0.0	2.124330799129733	0.0
48	0.0	0.0	0.0	2.3076735033123916	0.0
49	0.0	0.0	0.0	2.4005671400982718	0.0
50	0.0	0.0	0.0	2.4690150829931308	0.0
51	0.0	0.0	0.0	2.579020705502726	0.0
52	0.0	0.0	0.0	2.76969711785269	0.0
53	0.0	0.0	0.0	2.9359278363116337	0.0
54	0.0	0.0	0.0	3.07037915271225	0.0
55	0.0	0.0	0.0	3.16327278949813	0.0
56	0.0	0.0	0.0	3.346615493680788	0.0
57	0.0	0.0	0.0	3.498178795805119	0.0
58	0.0	0.0	0.0	3.635074681594837	0.0
59	0.0	0.0	0.0	3.752414012271738	0.0
60	0.0	0.0	0.0	3.896643606228763	0.0
61	0.0	0.0	0.0	3.9528687021781113	0.0
62	0.0	0.0	0.0	4.001760089960153	0.0
63	0.0	0.0	0.0	4.109321143080646	0.0
64	0.0	0.0	0.0	4.211993057422935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGAG	30	9.9978934E-8	70.0	29
TTGTAGA	15	0.002209553	70.0	36
GGATAGT	15	0.002209553	70.0	31
ATATCTA	15	0.002209553	70.0	33
GAGCGGC	15	0.002209553	70.0	26
AGGATAG	15	0.002209553	70.0	30
TTCGCGG	15	0.002209553	70.0	17
GGTGGTA	20	7.8376994E-5	69.99999	47
CTCTCGG	20	7.8376994E-5	69.99999	42
GCCGCTG	35	2.9050898E-7	60.0	14
CTTACGC	35	2.9050898E-7	60.0	12
TCTTACG	30	8.237101E-6	58.333332	11
TGTGTAG	30	8.237101E-6	58.333332	35
AGATCCC	25	2.3637828E-4	55.999996	40
TTAGATC	25	2.3637828E-4	55.999996	38
GAATAGA	25	2.3637828E-4	55.999996	26
TGTAGAG	25	2.3637828E-4	55.999996	37
GACGAGA	25	2.3637828E-4	55.999996	23
TCGCCCA	20	0.0069044377	52.499996	18
GAGGATA	20	0.0069044377	52.499996	29
>>END_MODULE