FastQCFastQC Report
Wed 25 May 2016
SRR1779865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40907
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2404558.77967096096023No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG31287.646613049111399No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC15843.8721979123377417No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT6091.4887427579631847RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC5511.3469577333952625No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT4481.0951670863177452No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT3800.9289363678588017No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC2490.6086977778864253No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1560.3813528246999291RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT1550.378908255310827No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1490.36424083897621434No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT1440.35201799203070383No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG1300.3177940205832743No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1040.2542352164666194No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT810.19801012051727088RNA PCR Primer, Index 24 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC760.18578727357176034No Hit
CTTATACACATCTCCGAGCCCACGAGAAGTGATAGCATCTCGTATGCCGT750.18334270418265822No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC740.1808981347935561No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG740.1808981347935561No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT580.14178502456792236No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCTAGCATCTCGTATGCCGT580.14178502456792236No Hit
TCTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT560.13689588578971815No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGT520.1271176082333097No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG480.11733933067690125No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCG470.11489476128779916No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTATAGCATCTCGTATGCCGT460.11245019189869705No Hit
CTTATACACCTCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT450.11000562250959493No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCC440.10756105312049281No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT420.10267191434228862No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATCGCATCTCGTATGCCGT420.10267191434228862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCG150.002209705869.9987836
AGCTCGT150.002209705869.9987837
GATAACA150.002209705869.9987831
GTCTGCC150.002209705869.9987842
TGATAAC150.002209705869.9987830
TAGCAGC150.002209705869.9987833
ATAACAT150.002209705869.9987832
TCTGCCG150.002209705869.9987843
ATAGCAG150.002209705869.9987832
GCTCGTA150.002209705869.9987838
TAACATC150.002209705869.9987833
GTGATAA150.002209705869.9987829
CTGCCGT150.002209705869.9987844
CGAGACA207.8383746E-569.9987823
CTTGAAC207.8383746E-569.9987857
TTTACAC252.792569E-669.998782
TCTTTAT800.065.704181
CTTTATA850.061.7636262
TTTATAC850.061.7636263
TGACAAA352.9054354E-759.99894759
Produced by FastQC (version 0.11.5)