##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779865_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40907
Sequences flagged as poor quality	0
Sequence length	76
%GC	43
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.600410687657366	34.0	34.0	34.0	34.0	34.0
2	33.692326496687606	34.0	34.0	34.0	34.0	34.0
3	33.793776126335345	34.0	34.0	34.0	34.0	34.0
4	33.57713838707312	34.0	34.0	34.0	34.0	34.0
5	33.664751753978535	34.0	34.0	34.0	34.0	34.0
6	37.394651282176646	38.0	38.0	38.0	37.0	38.0
7	37.44102476348791	38.0	38.0	38.0	37.0	38.0
8	37.3087491138436	38.0	38.0	38.0	37.0	38.0
9	37.27100496247586	38.0	38.0	38.0	37.0	38.0
10-11	37.18330603564182	38.0	38.0	38.0	36.5	38.0
12-13	37.33288923656098	38.0	38.0	38.0	36.5	38.0
14-15	37.36822548708045	38.0	38.0	38.0	37.0	38.0
16-17	37.0434277751974	38.0	38.0	38.0	35.0	38.0
18-19	36.81703620407265	38.0	38.0	38.0	34.0	38.0
20-21	37.01438629085487	38.0	38.0	38.0	35.0	38.0
22-23	37.17333219253429	38.0	38.0	38.0	36.0	38.0
24-25	37.111557924071676	38.0	38.0	38.0	35.5	38.0
26-27	36.89874593590339	38.0	38.0	38.0	35.0	38.0
28-29	36.29507174811157	38.0	37.0	38.0	34.0	38.0
30-31	36.369166646295255	38.0	37.0	38.0	33.0	38.0
32-33	36.62642090595742	38.0	37.0	38.0	34.5	38.0
34-35	36.552607133253474	38.0	37.0	38.0	34.5	38.0
36-37	36.409893172317695	38.0	37.0	38.0	34.0	38.0
38-39	32.88719534553988	36.5	32.5	37.5	18.5	38.0
40-41	35.4760432199868	37.5	35.5	38.0	30.5	38.0
42-43	36.8583005353607	38.0	37.0	38.0	34.5	38.0
44-45	36.643300657589165	38.0	37.0	38.0	34.5	38.0
46-47	36.820067470115134	38.0	37.0	38.0	34.5	38.0
48-49	36.280954359889506	38.0	37.0	38.0	34.0	38.0
50-51	36.22347031070477	38.0	37.0	38.0	33.5	38.0
52-53	36.11149680983695	38.0	37.0	38.0	33.0	38.0
54-55	36.19733786393527	38.0	37.0	38.0	34.0	38.0
56-57	36.04093431442051	38.0	37.0	38.0	33.0	38.0
58-59	35.87785709047351	38.0	37.0	38.0	32.0	38.0
60-61	35.33500378908255	38.0	36.5	38.0	29.0	38.0
62-63	35.39452905370719	38.0	36.0	38.0	29.0	38.0
64-65	36.12682425990661	38.0	37.0	38.0	34.0	38.0
66-67	35.56643117314886	38.0	36.5	38.0	31.0	38.0
68-69	35.21502432346542	38.0	36.0	38.0	30.0	38.0
70-71	30.496284254528568	33.5	27.5	37.5	17.5	38.0
72-73	21.959432370987848	22.5	11.0	28.0	10.0	37.5
74-75	20.701591414672308	21.0	11.0	25.0	10.5	37.0
76	20.886449751876206	21.0	11.0	27.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	6.0
24	15.0
25	36.0
26	84.0
27	162.0
28	307.0
29	561.0
30	845.0
31	1334.0
32	2013.0
33	3092.0
34	5653.0
35	12895.0
36	10451.0
37	3450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.836880473604384	4.183179216204316	13.207593326483682	76.77234698370762
2	2.6034663993937466	3.39550688146283	79.08915344562055	14.911873273522868
3	2.9677072383699614	5.705624954164324	82.91001540078715	8.416652406678564
4	2.092551397071406	69.12997775441856	22.174688928545237	6.602781919964798
5	2.0143251766201384	17.769574889383236	77.47818221820226	2.737917715794363
6	4.2804410003177935	77.73975114283618	12.811988168284158	5.16781968856186
7	3.9479795633999073	17.07042804410003	9.509374923607208	69.47221746889285
8	3.1388270956071085	77.96465152663359	4.752242892414501	14.144278485344808
9	3.466399393746791	14.290952648690933	2.9945975016500843	79.24805045591219
10-11	3.5128462121397317	44.8382917349109	38.324736597648325	13.324125455301047
12-13	4.3146649717652235	6.39621580658567	45.54966142713961	43.7394577945095
14-15	4.793800572029237	4.352555797296306	11.450363018554281	79.40328061212017
16-17	42.376610360085074	37.502138998215464	3.309946952844257	16.811303688855208
18-19	44.88962769208204	12.621311755934192	3.2647224191458677	39.2243381328379
20-21	7.515828586794436	4.534676216784414	3.2757229813968265	84.67377221502433
22-23	4.883027354731464	42.74207348375584	3.3160583763170117	49.05884078519569
24-25	42.47909842076957	41.536693883537865	3.3503642497433135	12.63384344594925
26-27	45.973794216148825	45.52277116385949	4.2376610360085065	4.265773583983181
28-29	41.3670032023859	12.673869997799889	4.540787640257168	41.41833915955704
30-31	45.17442002591244	5.522282249981666	42.673625540860975	6.629672183244921
32-33	12.5736426528467	42.4658371427873	40.563962158065856	4.396558046300144
34-35	37.01322512039504	46.07891069988022	12.719094531498277	4.188769648226465
36-37	11.605593174762266	40.82797565208888	5.094482606888796	42.47194856626005
38-39	4.455227711638595	12.62986774879605	40.80108538880876	42.11381915075659
40-41	4.174102231891852	4.33422152687804	46.001906764123504	45.489769477106606
42-43	42.22382477326619	3.837973940890312	42.07226147114186	11.86593981470164
44-45	7.185811719265652	41.28999926662919	46.015351895763565	5.508837118341605
46-47	42.10526315789473	6.553890532182756	13.167673014398515	38.173173295523995
48-49	40.00293348326692	3.5544038917544674	5.745960349084508	50.696702275894104
50-51	11.01902107476407	3.3873649210307564	43.80592636056917	41.787687643636005
52-53	3.5651408450704225	3.5113458528951487	80.26212832550861	12.661384976525822
54-55	3.4778780541492407	3.669870619023161	50.05502971604666	42.79722161078093
56-57	41.67267924620596	3.480366380101011	14.021743118144132	40.8252112555489
58-59	6.891239224796723	5.241792504738033	75.76572721159137	12.101241058873876
60-61	40.756816236703756	39.42168969311652	15.313608020540409	4.50788604963932
62-63	6.069904764233407	85.5165837377899	3.726298030490116	4.687213467486583
64-65	2.590401212684286	89.47702990146941	3.480355003545146	4.452213882301166
66-67	2.6715083935492547	89.38243529080928	3.337857169056964	4.608199146584504
68-69	2.833567630340444	89.08868651060449	3.3408715848664503	4.736874274188619
70-71	3.5413050387502136	82.47683543994329	4.455663398772706	9.526196122533799
72-73	6.58679706601467	66.0281173594132	7.6760391198044005	19.709046454767726
74-75	7.93398533007335	59.409535452322736	9.601466992665037	23.055012224938874
76	7.769791208253875	60.93834042345117	8.522810620507554	22.769057747787393
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	3.0
17	4.0
18	2.5
19	2.0
20	6.0
21	9.0
22	11.0
23	15.0
24	18.0
25	19.0
26	25.0
27	33.5
28	38.0
29	40.0
30	53.5
31	62.0
32	80.0
33	103.0
34	106.5
35	129.0
36	150.5
37	153.0
38	188.5
39	369.0
40	4133.5
41	9532.0
42	11311.0
43	10049.5
44	6887.0
45	3632.5
46	2279.0
47	1689.0
48	836.5
49	432.0
50	290.0
51	270.5
52	253.5
53	243.5
54	231.0
55	216.5
56	204.5
57	198.0
58	189.0
59	175.5
60	162.5
61	129.5
62	96.0
63	89.5
64	82.0
65	67.5
66	51.0
67	48.0
68	46.5
69	38.0
70	32.5
71	34.0
72	33.5
73	22.5
74	16.0
75	20.0
76	16.0
77	20.0
78	17.5
79	7.0
80	8.5
81	8.0
82	5.5
83	5.0
84	3.0
85	4.0
86	4.0
87	1.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07089251228396118
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0024445693891021096
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.012222846945510547
52-53	0.026890263280123203
54-55	0.04889138778204219
56-57	0.05011367247659325
58-59	0.03544625614198059
60-61	0.029334832669225312
62-63	0.02077883980736793
64-65	0.014667416334612656
66-67	0.030557117363776372
68-69	0.011000562250959495
70-71	0.009778277556408439
72-73	0.01711198572371477
74-75	0.01711198572371477
76	0.012222846945510547
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	40907.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.621653995648666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.64326812428078	7.397266971422983
2	11.990794016110472	2.547241303444398
3	6.812428078250863	2.170777617522673
4	4.188722669735328	1.7796465152663359
5	2.5086306098964326	1.3322903170606497
6	1.3118527042577675	0.8360427310729215
7	0.6444188722669736	0.4791356002640135
8	0.3222094361334868	0.2737917715794363
9	0.3682393555811277	0.35201799203070383
>10	1.6800920598388953	3.5397364754198546
>50	0.20713463751438435	1.4765199110176743
>100	0.20713463751438435	4.6813503801305405
>500	0.04602991944764096	2.8357004913584474
>1k	0.04602991944764096	11.518810961449141
>5k	0.0	0.0
>10k+	0.02301495972382048	58.77967096096023
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	24045	58.77967096096023	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	3128	7.646613049111399	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1584	3.8721979123377417	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	609	1.4887427579631847	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	551	1.3469577333952625	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	448	1.0951670863177452	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	380	0.9289363678588017	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	249	0.6086977778864253	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	156	0.3813528246999291	RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT	155	0.378908255310827	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	149	0.36424083897621434	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT	144	0.35201799203070383	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG	130	0.3177940205832743	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	104	0.2542352164666194	No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT	81	0.19801012051727088	RNA PCR Primer, Index 24 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	76	0.18578727357176034	No Hit
CTTATACACATCTCCGAGCCCACGAGAAGTGATAGCATCTCGTATGCCGT	75	0.18334270418265822	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	74	0.1808981347935561	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	74	0.1808981347935561	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT	58	0.14178502456792236	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCTAGCATCTCGTATGCCGT	58	0.14178502456792236	No Hit
TCTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	56	0.13689588578971815	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGT	52	0.1271176082333097	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG	48	0.11733933067690125	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCG	47	0.11489476128779916	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTATAGCATCTCGTATGCCGT	46	0.11245019189869705	No Hit
CTTATACACCTCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	45	0.11000562250959493	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCC	44	0.10756105312049281	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT	42	0.10267191434228862	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATCGCATCTCGTATGCCGT	42	0.10267191434228862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0024445693891021096	0.0
5	0.0	0.0	0.0	0.014667416334612658	0.0
6	0.0	0.0	0.0	0.014667416334612658	0.0
7	0.0	0.0	0.0	0.014667416334612658	0.0
8	0.0	0.0	0.0	0.014667416334612658	0.0
9	0.0	0.0	0.0	0.01711198572371477	0.0
10	0.0	0.0	0.0	0.01711198572371477	0.0
11	0.0	0.0	0.0	0.01711198572371477	0.0
12	0.0	0.0	0.0	0.01711198572371477	0.0
13	0.0	0.0	0.0	0.01711198572371477	0.0
14	0.0	0.0	0.0	0.01711198572371477	0.0
15	0.0	0.0	0.0	0.01711198572371477	0.0
16	0.0	0.0	0.0	0.019556555112816877	0.0
17	0.0	0.0	0.0	0.019556555112816877	0.0
18	0.0	0.0	0.0	0.026890263280123207	0.0
19	0.0	0.0	0.0	0.03666854083653164	0.0
20	0.0	0.0	0.0	0.04400224900383797	0.0
21	0.0	0.0	0.0	0.046446818392940084	0.0
22	0.0	0.0	0.0	0.05866966533845063	0.0
23	0.0	0.0	0.0	0.07333708167306328	0.0
24	0.0	0.0	0.0	0.07333708167306328	0.0
25	0.0	0.0	0.0	0.08067078984036961	0.0
26	0.0	0.0	0.0	0.09289363678588017	0.0
27	0.0	0.0	0.0	0.09533820617498227	0.0
28	0.0	0.0	0.0	0.11000562250959493	0.0
29	0.0	0.0	0.0	0.11733933067690126	0.0
30	0.0	0.0	0.0	0.1271176082333097	0.0
31	0.0	0.0	0.0	0.14422959395702448	0.0
32	0.0	0.0	0.0	0.16378614906984135	0.0
33	0.0	0.0	0.0	0.200454689906373	0.0
34	0.0	0.0	0.0	0.22245581440829199	0.0
35	0.0	0.0	0.0	0.2640134940230278	0.0
36	0.0	0.0	0.0	0.34468428386339744	0.0
37	0.0	0.0	0.0	0.5329161268242599	0.0
38	0.0	0.0	0.0	0.6575891656684675	0.0
39	0.0	0.0	0.0	0.7773730657344708	0.0
40	0.0	0.0	0.0	0.9411592148043122	0.0
41	0.0	0.0	0.0	1.1562813210452978	0.0
42	0.0	0.0	0.0	1.339624025227956	0.0
43	0.0	0.0	0.0	1.5034101742977974	0.0
44	0.0	0.0	0.0	1.6109712274182904	0.0
45	0.0	0.0	0.0	1.8089813479355612	0.0
46	0.0	0.0	0.0	1.9972131908964237	0.0
47	0.0	0.0	0.0	2.102329674627814	0.0
48	0.0	0.0	0.0	2.293006086977779	0.0
49	0.0	0.0	0.0	2.3907888625418634	0.0
50	0.0	0.0	0.0	2.447013958491212	0.0
51	0.0	0.0	0.0	2.5521304422226025	0.0
52	0.0	0.0	0.0	2.7036937443469333	0.0
53	0.0	0.0	0.0	2.8503679076930597	0.0
54	0.0	0.0	0.0	2.9725963771481654	0.0
55	0.0	0.0	0.0	3.060600875155841	0.0
56	0.0	0.0	0.0	3.236609871171193	0.0
57	0.0	0.0	0.0	3.3808394651282176	0.0
58	0.0	0.0	0.0	3.500623365194221	0.0
59	0.0	0.0	0.0	3.6179626958711224	0.0
60	0.0	0.0	0.0	3.7621922898281466	0.0
61	0.0	0.0	0.0	3.820861955166597	0.0
62	0.0	0.0	0.0	3.8624196347813333	0.0
63	0.0	0.0	0.0	3.97486982668003	0.0
64	0.0	0.0	0.0	4.060429755298604	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCTCG	15	0.0022097058	69.99878	36
AGCTCGT	15	0.0022097058	69.99878	37
GATAACA	15	0.0022097058	69.99878	31
GTCTGCC	15	0.0022097058	69.99878	42
TGATAAC	15	0.0022097058	69.99878	30
TAGCAGC	15	0.0022097058	69.99878	33
ATAACAT	15	0.0022097058	69.99878	32
TCTGCCG	15	0.0022097058	69.99878	43
ATAGCAG	15	0.0022097058	69.99878	32
GCTCGTA	15	0.0022097058	69.99878	38
TAACATC	15	0.0022097058	69.99878	33
GTGATAA	15	0.0022097058	69.99878	29
CTGCCGT	15	0.0022097058	69.99878	44
CGAGACA	20	7.8383746E-5	69.99878	23
CTTGAAC	20	7.8383746E-5	69.99878	57
TTTACAC	25	2.792569E-6	69.99878	2
TCTTTAT	80	0.0	65.70418	1
CTTTATA	85	0.0	61.763626	2
TTTATAC	85	0.0	61.763626	3
TGACAAA	35	2.9054354E-7	59.998947	59
>>END_MODULE