FastQCFastQC Report
Wed 25 May 2016
SRR1779864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346650
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC308.367799E-658.33333215
TGGACGC200.00694399852.50757649
GCGCATT200.006947961652.544
GTGTCGA200.006947961652.536
GCGTCGG200.006947961652.536
CTCGGTG2050.044.39024444
TGGTCGC1550.042.90941649
CGGTGGT1900.042.3684246
GTAGATC2900.041.0344838
GATCTCG2050.040.9756141
ACGAATA602.5928057E-740.83333224
ATCTCGG2450.040.042
AGTTTCG350.001254555840.037
GACGTAA350.001254555840.068
TAGATCT2450.040.039
TGGGATA350.001254555840.061
GACGAAT701.9215804E-840.023
AGATCTC2750.039.45454840
GTCGCCG1600.039.3806851
TCGGTGG2400.039.37545
Produced by FastQC (version 0.11.5)