##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779864_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346650
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.6564402134718	34.0	31.0	34.0	12.0	34.0
2	30.40518101831819	34.0	32.0	34.0	12.0	34.0
3	29.932349632193855	34.0	31.0	34.0	11.0	34.0
4	30.03820279821145	34.0	31.0	34.0	12.0	34.0
5	30.648244627145537	34.0	32.0	34.0	21.0	34.0
6	33.38580124044425	38.0	34.0	38.0	12.0	38.0
7	33.58394922832829	38.0	34.0	38.0	21.0	38.0
8	32.890783210731286	38.0	34.0	38.0	11.0	38.0
9	33.7316255589211	38.0	34.0	38.0	21.0	38.0
10-11	32.315681523150154	37.0	30.5	38.0	16.5	38.0
12-13	33.20450887061878	38.0	34.0	38.0	16.0	38.0
14-15	31.954878119140343	37.0	29.0	38.0	11.0	38.0
16-17	32.58344439636521	37.5	31.0	38.0	16.0	38.0
18-19	33.48574498773979	38.0	34.0	38.0	21.0	38.0
20-21	32.16999711524593	37.0	30.0	38.0	16.0	38.0
22-23	33.055142074138175	38.0	32.5	38.0	16.0	38.0
24-25	33.39447569594692	38.0	34.0	38.0	21.0	38.0
26-27	32.27998557622963	37.0	31.0	38.0	11.0	38.0
28-29	33.49970863983845	38.0	34.0	38.0	21.0	38.0
30-31	33.87513486225299	38.0	35.0	38.0	21.0	38.0
32-33	33.99427520553873	38.0	36.0	38.0	21.0	38.0
34-35	33.92837155632482	38.0	35.5	38.0	21.0	38.0
36-37	33.44958171065916	38.0	34.0	38.0	16.0	38.0
38-39	33.41270157219097	38.0	34.0	38.0	16.0	38.0
40-41	33.43350064906967	38.0	34.0	38.0	16.0	38.0
42-43	33.76958026828213	38.0	35.0	38.0	21.0	38.0
44-45	33.82951968844656	38.0	35.0	38.0	21.0	38.0
46-47	33.75774412231357	38.0	34.5	38.0	21.0	38.0
48-49	33.47911149574499	38.0	34.0	38.0	16.0	38.0
50-51	32.87900331746719	37.5	32.5	38.0	11.0	38.0
52-53	33.42501658733593	38.0	34.0	38.0	21.0	38.0
54-55	33.55333333333333	38.0	34.0	38.0	21.0	38.0
56-57	33.61827058993221	38.0	34.0	38.0	21.0	38.0
58-59	33.82946632049618	38.0	35.0	38.0	21.0	38.0
60-61	33.725166594547815	38.0	34.5	38.0	21.0	38.0
62-63	33.73696812346748	38.0	34.5	38.0	21.0	38.0
64-65	33.721495744987735	38.0	34.5	38.0	21.0	38.0
66-67	33.547097937400835	38.0	34.0	38.0	21.0	38.0
68-69	32.95941006779172	38.0	33.0	38.0	11.0	38.0
70-71	32.99709937977787	37.0	33.5	38.0	11.0	38.0
72-73	33.231549112938126	37.5	34.0	38.0	15.0	38.0
74-75	33.323445838742245	38.0	34.0	38.0	15.0	38.0
76	32.457605654117984	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	112.0
13	1000.0
14	2723.0
15	4383.0
16	5545.0
17	6580.0
18	6878.0
19	6245.0
20	5131.0
21	3977.0
22	3219.0
23	2754.0
24	2651.0
25	2787.0
26	3132.0
27	3780.0
28	4350.0
29	5406.0
30	6582.0
31	8406.0
32	10722.0
33	14337.0
34	20691.0
35	31373.0
36	57888.0
37	125994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.29983643397212	20.551047312988064	11.800986011787138	29.34813024125268
2	18.915909418722055	21.753065051204384	34.67791720755805	24.653108322515504
3	17.597339095165427	22.819047509151908	31.36198287028089	28.221630525401775
4	13.063031876532524	22.6764748305207	33.52891965959902	30.731573633347757
5	12.99697100822155	36.12779460550988	34.43502091446704	16.440213471801528
6	27.276189047521516	39.31595330065802	17.49609829132566	15.911759360494804
7	25.61708093886312	29.543435112742046	22.62947448591013	22.21000946248471
8	22.006277369735923	39.01373751593864	18.57507168779317	20.40491342653227
9	23.495182043736655	18.266112745946568	20.64537533898794	37.593329871328834
10-11	23.64621407285389	26.82949696946968	24.81457654793604	24.70971240974039
12-13	23.422506018326867	24.07878851693137	25.67117506299591	26.827530401745857
14-15	24.07380963713964	25.355944133610848	22.813887843161012	27.756358386088504
16-17	24.581348857201377	26.762084990869727	23.498841767897787	25.15772438403111
18-19	21.937027704175314	26.5909310547261	25.889347732330965	25.58269350876763
20-21	23.93336218087408	25.893119861531805	25.351939997115245	24.82157796047887
22-23	24.558804650887726	26.080108238002442	24.435623342136807	24.92546376897302
24-25	21.907954425800572	28.120677295261558	24.4199011376241	25.551467141313772
26-27	23.483408289076273	27.88757503988184	24.753857648514778	23.87515902252711
28-29	22.387736762162483	27.62070164253187	25.928260577936964	24.063301017368687
30-31	22.866279338118208	27.083092935773468	25.312709145877086	24.737918580231245
32-33	21.93307370546661	27.730708207125343	25.627722486658012	24.708495600750034
34-35	21.95790739974123	27.579725969700597	26.07860304840317	24.383763582154998
36-37	23.77154892597989	26.113908163706924	25.62428771332535	24.49025519698784
38-39	23.14852613433923	26.16376605673319	26.65089542658079	24.03681238234679
40-41	22.016376914233955	26.83800355693769	26.348023130191706	24.79759639863665
42-43	21.12570010082317	26.718717771562673	26.89843976500556	25.257142362608594
44-45	20.83900691214992	25.53730714640141	27.59696050035195	26.02672544109671
46-47	21.252277215155182	25.937967433118747	27.20743825375281	25.60231709797326
48-49	21.200293669235553	26.02333487668344	27.64875659349456	25.127614860586444
50-51	23.037967015055834	25.860611637909898	26.39112862512729	24.710292721906978
52-53	19.69086738888969	26.924541638417267	27.429568829969853	25.955022142723195
54-55	20.11632368681463	26.57644272241279	26.752139957705722	26.555093633066857
56-57	20.054038302856846	26.862056629140866	26.659521185438223	26.42438388256406
58-59	19.98346852311594	26.876344264399766	26.924525008547036	26.215662203937264
60-61	19.615129932238748	26.435833108520434	26.765478548521354	27.183558410719467
62-63	20.178381840422833	26.632498211277035	26.22917003254322	26.95994991575692
64-65	20.694261708561275	26.161911686632966	25.83414121829051	27.30968538651525
66-67	20.189501534218042	27.398234531230393	25.173005264091795	27.239258670459776
68-69	20.288795229729377	28.388245102366383	24.228249487230524	27.094710180673708
70-71	21.169024485933857	28.30104313309178	24.128222288892477	26.40171009208188
72-73	21.658290877409602	27.15602047115978	24.082028883490946	27.103659767939668
74-75	21.613489065161858	27.37265287929341	24.427653795278133	26.586204260266598
76	21.637207584836098	27.519119314329892	24.312613410493338	26.53105969034067
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	5.0
14	6.5
15	15.0
16	38.5
17	51.0
18	77.0
19	124.0
20	188.0
21	231.0
22	314.0
23	495.0
24	748.5
25	904.0
26	1031.5
27	1404.5
28	1888.0
29	2126.0
30	2453.5
31	3415.0
32	4716.0
33	5383.0
34	6076.5
35	7485.0
36	9293.0
37	10386.0
38	11157.5
39	13455.0
40	16077.5
41	17628.5
42	18083.0
43	19134.5
44	20921.0
45	21608.0
46	21560.0
47	21873.5
48	21316.0
49	19832.0
50	19219.0
51	18384.5
52	16281.0
53	14066.5
54	13121.0
55	12383.0
56	10678.5
57	8857.5
58	8003.0
59	7145.5
60	5665.5
61	4577.0
62	4111.0
63	3824.0
64	3277.5
65	2860.0
66	2478.5
67	2255.0
68	2121.5
69	1884.5
70	1648.5
71	1516.0
72	1373.0
73	1177.0
74	1023.0
75	922.0
76	857.0
77	746.0
78	621.0
79	542.0
80	487.5
81	390.5
82	281.5
83	215.0
84	196.0
85	153.5
86	112.5
87	95.0
88	82.5
89	58.5
90	41.5
91	34.0
92	32.0
93	26.0
94	20.0
95	16.0
96	12.0
97	10.0
98	10.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.88475407471513E-4
2	0.0
3	2.88475407471513E-4
4	0.0
5	0.0
6	0.0020193278523005912
7	0.005192557334487234
8	0.002307803259772104
9	0.008654262224145393
10-11	0.0031732294821866435
12-13	1.442377037357565E-4
14-15	4.3271311120726956E-4
16-17	2.88475407471513E-4
18-19	0.0015866147410933218
20-21	0.0
22-23	0.001875090148564835
24-25	0.001875090148564835
26-27	2.88475407471513E-4
28-29	0.005913745853166018
30-31	5.76950814943026E-4
32-33	0.0
34-35	0.002740516370979374
36-37	0.01586614741093322
38-39	0.00793307370546661
40-41	0.0010096639261502956
42-43	0.0010096639261502956
44-45	0.0040386557046011825
46-47	0.002740516370979374
48-49	4.3271311120726956E-4
50-51	0.0020193278523005912
52-53	0.010096639261502957
54-55	0.008942737631616904
56-57	0.012692917928746574
58-59	0.011106303187653254
60-61	0.018895139189384104
62-63	0.009808163854031444
64-65	0.019039376893119863
66-67	0.01629886052214049
68-69	8.654262224145391E-4
70-71	5.76950814943026E-4
72-73	0.004615606519544208
74-75	0.008221549112938122
76	0.0031732294821866435
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	346650.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.92430140292862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37870129705084	44.04728514800505
2	8.801557387318145	10.02045011061742
3	5.1659197369458365	8.821991163877275
4	3.060841503639296	6.9694505719902615
5	1.7066533416714813	4.857502460581137
6	1.0282357381590277	3.5118960643336377
7	0.6073636423548333	2.4201625727010954
8	0.39635984313126016	1.805000573953709
9	0.2883214849364906	1.4771249198518264
>10	1.5369165929821171	14.985106105546524
>50	0.027568926535109676	0.9889814888092143
>100	0.0015605052755721979	0.09504881973285921
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0011539016298860523	0.0
2	0.0	0.0	0.0	0.0014423770373575653	0.0
3	0.0	0.0	0.0	0.0014423770373575653	0.0
4	0.0	0.0	0.0	0.0066349343718448	0.0
5	0.0	0.0	0.0	0.010673590076445983	0.0
6	0.0	0.0	0.0	0.010673590076445983	0.0
7	0.0	0.0	0.0	0.01125054089138901	0.0
8	0.0	0.0	0.0	0.01125054089138901	0.0
9	0.0	0.0	0.0	0.014712245781047165	0.0
10	0.0	0.0	0.0	0.015577672003461706	0.0
11	0.0	0.0	0.0	0.015577672003461706	0.0
12	0.0	0.0	0.0	0.016443098225876245	0.0
13	0.0	0.0	0.0	0.016731573633347756	0.0
14	0.0	0.0	0.0	0.018462426078176837	0.0
15	0.0	0.0	0.0	0.018462426078176837	0.0
16	0.0	0.0	0.0	0.020481753930477426	0.0
17	0.0	0.0	0.0	0.020481753930477426	0.0
18	0.0	0.0	0.0	0.02250108178277802	0.0
19	0.0	0.0	0.0	0.02365498341266407	0.0
20	0.0	0.0	0.0	0.025674311264964663	0.0
21	0.0	0.0	0.0	0.026828212894850715	0.0
22	0.0	0.0	0.0	0.037213327563825185	0.0
23	0.0	0.0	0.0	0.0375018029712967	0.0
24	0.0	0.0	0.0	0.04154045867589788	0.0
25	0.0	0.0	0.0	0.042117409490840904	0.0
26	0.0	0.0	0.0	0.04529063897302755	0.0
27	0.0	0.0	0.0	0.06057983556901774	0.0
28	0.0	0.0	0.0	0.08452329438915332	0.0
29	0.0	0.0	0.0	0.11135150728400404	0.0
30	0.0	0.0	0.0	0.14019904803115535	0.0
31	0.0	0.0	0.0	0.18808596567142652	0.0
32	0.0	0.0	0.0	0.22789557190249532	0.0
33	0.0	0.0	0.0	0.2870330304341555	0.0
34	0.0	0.0	0.0	0.36347901341410643	0.0
35	0.0	0.0	0.0	0.47396509447569596	0.0
36	0.0	0.0	0.0	0.674167027260926	0.0
37	0.0	0.0	0.0	0.9646617625847397	0.0
38	0.0	0.0	0.0	1.3310255300735612	0.0
39	0.0	0.0	0.0	1.7709505264676186	0.0
40	0.0	0.0	0.0	2.2953988172508293	0.0
41	0.0	0.0	0.0	2.958603779027838	0.0
42	0.0	0.0	0.0	3.723352084234819	0.0
43	0.0	0.0	0.0	4.466176258473965	0.0
44	0.0	0.0	0.0	5.209288908120583	0.0
45	0.0	0.0	0.0	5.989326409923554	0.0
46	0.0	0.0	0.0	6.731862108755228	0.0
47	0.0	0.0	0.0	7.429107168613876	0.0
48	0.0	0.0	0.0	8.16962353959325	0.0
49	0.0	0.0	0.0	8.775421895283428	0.0
50	0.0	0.0	0.0	9.487956151738064	0.0
51	0.0	0.0	0.0	10.1944324246358	0.0
52	0.0	0.0	0.0	10.965527188807155	0.0
53	0.0	0.0	0.0	11.695658445117553	0.0
54	0.0	0.0	0.0	12.328285013702581	0.0
55	0.0	0.0	0.0	13.03706908986009	0.0
56	0.0	0.0	0.0	13.665657002740517	0.0
57	0.0	0.0	0.0	14.247511899610558	0.0
58	0.0	0.0	0.0	14.802538583585749	0.0
59	0.0	0.0	0.0	15.336218087408048	0.0
60	0.0	0.0	0.0	15.908264820424058	0.0
61	0.0	0.0	0.0	16.46906101254868	0.0
62	0.0	0.0	0.0	17.035915188230202	0.0
63	0.0	0.0	0.0	17.625270445694504	0.0
64	0.0	0.0	0.0	18.204529063897304	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGC	30	8.367799E-6	58.333332	15
TGGACGC	20	0.006943998	52.507576	49
GCGCATT	20	0.0069479616	52.5	44
GTGTCGA	20	0.0069479616	52.5	36
GCGTCGG	20	0.0069479616	52.5	36
CTCGGTG	205	0.0	44.390244	44
TGGTCGC	155	0.0	42.909416	49
CGGTGGT	190	0.0	42.36842	46
GTAGATC	290	0.0	41.03448	38
GATCTCG	205	0.0	40.97561	41
ACGAATA	60	2.5928057E-7	40.833332	24
ATCTCGG	245	0.0	40.0	42
AGTTTCG	35	0.0012545558	40.0	37
GACGTAA	35	0.0012545558	40.0	68
TAGATCT	245	0.0	40.0	39
TGGGATA	35	0.0012545558	40.0	61
GACGAAT	70	1.9215804E-8	40.0	23
AGATCTC	275	0.0	39.454548	40
GTCGCCG	160	0.0	39.38068	51
TCGGTGG	240	0.0	39.375	45
>>END_MODULE