FastQCFastQC Report
Wed 25 May 2016
SRR1779864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346650
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT310148.946776287321507No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG37521.082359728833117No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC21540.6213760276936391No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT8480.24462714553584305TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6760.19500937545074282No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT3810.10990913024664647No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT3760.10846675320928892No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGT150.002225662869.9835612
TCTCGTA45450.054.12369538
ATCTCGT45650.054.0398837
AATCTCG45600.053.7154536
CCGTCTT47500.053.6527247
TGCCGTC47850.053.61060745
CGTATGC47650.053.6075541
TATGCCG47650.053.6075543
ATGCCGT47700.053.5513644
TCGTATG47800.053.5125340
CGTCTTC47450.053.49564748
GCCGTCT47850.053.4797646
CTCGTAT47400.053.4473639
CTGCTTG47800.053.25812554
GTCTTCT48050.052.9081549
TCTTCTG48100.052.85315350
GTATGCC48300.052.74123442
GATGGTC200.00693460452.52554766
ATCCGAA200.006942525552.5103946
CGGCATG200.006954420452.48766734