##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779864_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346650
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.533823741526035	34.0	34.0	34.0	34.0	34.0
2	33.63714120871196	34.0	34.0	34.0	34.0	34.0
3	33.75148997547959	34.0	34.0	34.0	34.0	34.0
4	33.54982547237848	34.0	34.0	34.0	34.0	34.0
5	33.61336506562815	34.0	34.0	34.0	34.0	34.0
6	37.272741958748014	38.0	38.0	38.0	36.0	38.0
7	37.31776143083802	38.0	38.0	38.0	37.0	38.0
8	37.212776575796916	38.0	38.0	38.0	36.0	38.0
9	37.14847829222559	38.0	38.0	38.0	36.0	38.0
10-11	37.098233088129234	38.0	37.5	38.0	35.5	38.0
12-13	37.25190538006635	38.0	38.0	38.0	36.5	38.0
14-15	37.27255300735612	38.0	38.0	38.0	36.0	38.0
16-17	37.1937415260349	38.0	38.0	38.0	36.0	38.0
18-19	37.063359296120005	38.0	38.0	38.0	35.5	38.0
20-21	37.077012837155635	38.0	38.0	38.0	36.0	38.0
22-23	37.07294821866436	38.0	38.0	38.0	36.0	38.0
24-25	37.251009663926155	38.0	38.0	38.0	36.0	38.0
26-27	37.1768109043704	38.0	38.0	38.0	36.0	38.0
28-29	36.68475407471513	38.0	37.0	38.0	34.0	38.0
30-31	36.933142939564405	38.0	37.5	38.0	36.0	38.0
32-33	37.00784797346026	38.0	38.0	38.0	36.0	38.0
34-35	37.04685129092745	38.0	38.0	38.0	36.0	38.0
36-37	37.03580124044425	38.0	38.0	38.0	36.0	38.0
38-39	36.622710226453194	38.0	37.0	38.0	34.0	38.0
40-41	36.87287321505842	38.0	37.5	38.0	35.0	38.0
42-43	37.16095918072984	38.0	38.0	38.0	36.0	38.0
44-45	36.94112793884321	38.0	38.0	38.0	35.5	38.0
46-47	37.07070387999423	38.0	38.0	38.0	36.0	38.0
48-49	36.72274195874802	38.0	37.0	38.0	34.5	38.0
50-51	36.88958459541324	38.0	37.5	38.0	35.5	38.0
52-53	36.81160248088851	38.0	37.0	38.0	35.0	38.0
54-55	36.81654406461849	38.0	37.0	38.0	35.0	38.0
56-57	36.69230924563681	38.0	37.0	38.0	34.5	38.0
58-59	36.78697245059858	38.0	37.0	38.0	35.0	38.0
60-61	36.47322515505553	38.0	37.0	38.0	34.0	38.0
62-63	36.29337227751334	38.0	37.0	38.0	34.0	38.0
64-65	36.64021202942449	38.0	37.0	38.0	34.0	38.0
66-67	36.43977498918217	38.0	37.0	38.0	34.0	38.0
68-69	36.52498485504111	38.0	37.0	38.0	34.0	38.0
70-71	35.82384249242752	38.0	37.0	38.0	31.0	38.0
72-73	34.60800230780326	38.0	37.0	38.0	23.0	38.0
74-75	34.420800519255735	38.0	36.5	38.0	23.0	38.0
76	34.17724794461272	38.0	36.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	1.0
21	5.0
22	10.0
23	43.0
24	96.0
25	161.0
26	291.0
27	611.0
28	1084.0
29	1780.0
30	2831.0
31	4692.0
32	7286.0
33	11843.0
34	19897.0
35	37550.0
36	56403.0
37	202065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.78430625544761	17.87827361883157	11.636968154189828	33.70045197153099
2	18.12808308091735	18.382518390307226	40.51781335641137	22.971585172364055
3	17.1210154334343	21.04283859800952	36.973604500216354	24.862541468339824
4	12.19991345737776	25.46458964373287	35.39189384105005	26.943603057839322
5	12.192990047598443	32.45204096350786	41.79575941151017	13.559209577383527
6	25.27534977643156	43.92730419731718	17.83585749314871	12.961488533102553
7	24.09952401557767	27.865858935525743	21.447569594692055	26.587047454204527
8	21.47180152891966	41.83037646040675	18.1488533102553	18.54896870041829
9	21.59959613442954	16.53339102841483	19.373142939564403	42.49386989759123
10-11	22.822731862108757	28.312707341699124	28.444684840617334	20.41987595557479
12-13	22.6349343718448	20.833693927592673	28.881580845232946	27.64979085532958
14-15	20.63450165873359	21.249242752055387	21.87566709937978	36.24058848983125
16-17	26.315736333477567	28.641136593105436	22.34804557911438	22.69508149430261
18-19	26.19803836722919	24.581133708351363	23.379922111639985	25.840905812779464
20-21	20.722486658012404	23.450887061877975	22.844944468484062	32.981681811625556
22-23	20.80109620654839	28.99567286888793	23.084234818981685	27.118996105582
24-25	26.171512147839582	29.029038269589147	23.06344224367222	21.73600733889905
26-27	25.89802394345882	29.97331602480888	23.512476561373145	20.61618347035915
28-29	20.475407471513055	24.242175104572336	29.055675753642003	26.226741670272606
30-31	20.3731429395644	28.743833838165294	24.97692196740228	25.906101254868023
32-33	19.756815231501516	24.392615029568727	34.1888071541901	21.66176258473965
34-35	26.135583441511614	28.173373719890378	25.48564834847829	20.20539449011972
36-37	20.950959180729843	35.03144381941439	23.29813933362181	20.719457666233954
38-39	19.92990047598442	25.112361171210157	29.367229193711236	25.59050915909419
40-41	19.945478147987885	22.512043848261936	30.441655848838888	27.10082215491129
42-43	25.646510380081324	22.145279309504268	29.964272269251797	22.243938041162615
44-45	19.852589066782055	28.012692917928746	30.977066205106013	21.157651810183182
46-47	25.045867589787967	22.469637963363624	26.28616760421174	26.198326842636664
48-49	24.277080628876387	22.43920380787538	25.196884465599307	28.086831097648922
50-51	19.784160726831036	22.38744606542691	31.06256338349195	26.76582982425011
52-53	18.225743854176734	22.463148144674822	36.70281757808611	22.60829042306233
54-55	17.727691210976396	22.779222428417643	31.946324414339273	27.54676194626669
56-57	23.33801849436049	22.547938721557127	26.57063529629074	27.543407487791644
58-59	18.225247699750533	22.55506963872701	35.8026597333341	23.417022928188356
60-61	23.25294512296294	27.954753677345817	26.25224536318975	22.54005583650149
62-63	17.98905233755966	34.89762175451151	24.02628131510454	23.087044592824288
64-65	18.041101779269077	35.670204816461485	23.05952343170717	23.229169972562268
66-67	17.8665470080784	35.57712424053108	22.741432122848202	23.814896628542325
68-69	17.868316853106002	35.8715047132729	22.581445499852144	23.67873293376896
70-71	18.34353925493166	34.705182300119006	22.629449312993614	24.321829131955713
72-73	18.659157107800354	32.15674280578394	22.805524257712133	26.378575828703575
74-75	18.905074320269584	31.36828924894982	23.347124128698702	26.379512302081892
76	18.822324481052465	31.39852574181729	23.289817232375977	26.48933254475427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	3.5
11	5.0
12	3.0
13	10.0
14	19.5
15	27.0
16	47.5
17	63.0
18	95.0
19	125.0
20	183.0
21	243.0
22	310.0
23	509.0
24	783.0
25	925.0
26	1027.0
27	1400.0
28	2058.5
29	2446.0
30	2635.0
31	3181.0
32	4403.5
33	5269.0
34	5791.0
35	6882.5
36	8792.0
37	10132.0
38	15251.5
39	24144.0
40	27547.5
41	25133.0
42	23088.0
43	21747.5
44	20428.0
45	19812.5
46	19176.0
47	19284.0
48	18566.5
49	16880.0
50	16019.0
51	15330.5
52	13225.0
53	11186.0
54	10564.0
55	9903.5
56	8370.5
57	6760.5
58	6023.0
59	5352.0
60	4283.5
61	3501.0
62	3116.0
63	2941.5
64	2606.0
65	2286.5
66	1993.5
67	1859.0
68	1832.0
69	1683.0
70	1445.5
71	1330.0
72	1200.0
73	1025.0
74	924.5
75	869.0
76	803.0
77	701.0
78	581.5
79	498.0
80	462.5
81	354.5
82	251.5
83	221.0
84	196.0
85	141.5
86	91.5
87	71.0
88	56.0
89	32.0
90	18.5
91	13.0
92	12.0
93	8.5
94	4.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04846386845521419
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001153901629886052
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.442377037357565E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014279532669839896
52-53	0.02697245059858647
54-55	0.0464445406029136
56-57	0.047886917640271164
58-59	0.032165007933073704
60-61	0.02956872926583009
62-63	0.0265397374873792
64-65	0.013558344151161115
66-67	0.03129958171065916
68-69	0.006490696668109044
70-71	0.010817827780181739
72-73	0.020049040819270156
74-75	0.012115967113803548
76	0.010096639261502957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	346650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.554593970863984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.869837848429924	9.155632482330882
2	15.326017733797089	7.219962498197028
3	12.930485474942438	9.137170056252703
4	9.801352079557144	9.234674743978076
5	6.893891147797972	8.119140343285734
6	4.313427717630922	6.096062310688014
7	2.8474501543134276	4.694937256598875
8	1.7721549992651742	3.339391316890235
9	1.1928672904521629	2.5287754218952836
>10	5.795326507617695	24.833982403000142
>50	0.21432420516337627	3.2482330881292367
>100	0.03674129231372165	1.3018895139189386
>500	0.002449419487581443	0.4396365209865859
>1k	0.002449419487581443	1.7037357565267561
>5k	0.0	0.0
>10k+	0.0012247097437907215	8.946776287321507
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	31014	8.946776287321507	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	3752	1.082359728833117	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	2154	0.6213760276936391	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	848	0.24462714553584305	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	676	0.19500937545074282	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	381	0.10990913024664647	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	376	0.10846675320928892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0037501802971296695	0.0
2	0.0	0.0	0.0	0.0040386557046011825	0.0
3	0.0	0.0	0.0	0.0040386557046011825	0.0
4	0.0	0.0	0.0	0.01038511466897447	0.0
5	0.0	0.0	0.0	0.015289196595990193	0.0
6	0.0	0.0	0.0	0.015289196595990193	0.0
7	0.0	0.0	0.0	0.015289196595990193	0.0
8	0.0	0.0	0.0	0.015289196595990193	0.0
9	0.0	0.0	0.0	0.020481753930477426	0.0
10	0.0	0.0	0.0	0.021635655560363478	0.0
11	0.0	0.0	0.0	0.021635655560363478	0.0
12	0.0	0.0	0.0	0.02278955719024953	0.0
13	0.0	0.0	0.0	0.02365498341266407	0.0
14	0.0	0.0	0.0	0.024808885042550122	0.0
15	0.0	0.0	0.0	0.025097360450021637	0.0
16	0.0	0.0	0.0	0.027116688302322226	0.0
17	0.0	0.0	0.0	0.027116688302322226	0.0
18	0.0	0.0	0.0	0.029712966969565845	0.0
19	0.0	0.0	0.0	0.030866868599451897	0.0
20	0.0	0.0	0.0	0.03346314726669551	0.0
21	0.0	0.0	0.0	0.03461704889658156	0.0
22	0.0	0.0	0.0	0.0375018029712967	0.0
23	0.0	0.0	0.0	0.03779027837876821	0.0
24	0.0	0.0	0.0	0.03894418000865426	0.0
25	0.0	0.0	0.0	0.03952113082359729	0.0
26	0.0	0.0	0.0	0.043271311120726956	0.0
27	0.0	0.0	0.0	0.06029136016154623	0.0
28	0.0	0.0	0.0	0.08510024520409636	0.0
29	0.0	0.0	0.0	0.1148132121736622	0.0
30	0.0	0.0	0.0	0.14423770373575653	0.0
31	0.0	0.0	0.0	0.19529785085821433	0.0
32	0.0	0.0	0.0	0.23510745708928313	0.0
33	0.0	0.0	0.0	0.3000144237703736	0.0
34	0.0	0.0	0.0	0.38136448867734024	0.0
35	0.0	0.0	0.0	0.4984855041107746	0.0
36	0.0	0.0	0.0	0.718015289196596	0.0
37	0.0	0.0	0.0	1.0298572046733017	0.0
38	0.0	0.0	0.0	1.4103562671282273	0.0
39	0.0	0.0	0.0	1.856050771671715	0.0
40	0.0	0.0	0.0	2.4030001442377036	0.0
41	0.0	0.0	0.0	3.087840761575076	0.0
42	0.0	0.0	0.0	3.87220539449012	0.0
43	0.0	0.0	0.0	4.639261502956873	0.0
44	0.0	0.0	0.0	5.402855906533968	0.0
45	0.0	0.0	0.0	6.201644309822588	0.0
46	0.0	0.0	0.0	6.981970287033031	0.0
47	0.0	0.0	0.0	7.697389297562383	0.0
48	0.0	0.0	0.0	8.471368815808452	0.0
49	0.0	0.0	0.0	9.106591663060724	0.0
50	0.0	0.0	0.0	9.839030722630895	0.0
51	0.0	0.0	0.0	10.559930765902207	0.0
52	0.0	0.0	0.0	11.328717726813789	0.0
53	0.0	0.0	0.0	12.056829655271889	0.0
54	0.0	0.0	0.0	12.69032165007933	0.0
55	0.0	0.0	0.0	13.386412808308092	0.0
56	0.0	0.0	0.0	14.022212606375307	0.0
57	0.0	0.0	0.0	14.606086831097649	0.0
58	0.0	0.0	0.0	15.158805711813068	0.0
59	0.0	0.0	0.0	15.708639838453772	0.0
60	0.0	0.0	0.0	16.2855906533968	0.0
61	0.0	0.0	0.0	16.859079763450165	0.0
62	0.0	0.0	0.0	17.438915332467907	0.0
63	0.0	0.0	0.0	18.0475984422328	0.0
64	0.0	0.0	0.0	18.637530650512044	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGT	15	0.0022256628	69.98356	12
TCTCGTA	4545	0.0	54.123695	38
ATCTCGT	4565	0.0	54.03988	37
AATCTCG	4560	0.0	53.71545	36
CCGTCTT	4750	0.0	53.65272	47
TGCCGTC	4785	0.0	53.610607	45
CGTATGC	4765	0.0	53.60755	41
TATGCCG	4765	0.0	53.60755	43
ATGCCGT	4770	0.0	53.55136	44
TCGTATG	4780	0.0	53.51253	40
CGTCTTC	4745	0.0	53.495647	48
GCCGTCT	4785	0.0	53.47976	46
CTCGTAT	4740	0.0	53.44736	39
CTGCTTG	4780	0.0	53.258125	54
GTCTTCT	4805	0.0	52.90815	49
TCTTCTG	4810	0.0	52.853153	50
GTATGCC	4830	0.0	52.741234	42
GATGGTC	20	0.006934604	52.525547	66
ATCCGAA	20	0.0069425255	52.51039	46
CGGCATG	20	0.0069544204	52.487667	34
>>END_MODULE